NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 297-421.
STRAIN=BCG / Pasteur;
DOI=10.1111/j.1365-2958.1994.tb00342.x; PubMed=7910936 [NCBI, ExPASy, EBI, Israel, Japan]
Cirillo J.D., Weisbrod T.R., Pascopella L., Bloom B.R., Jacobs W.R. Jr.;
"Isolation and characterization of the aspartokinase and aspartate semialdehyde dehydrogenase operon from mycobacteria.";
Mol. Microbiol. 11:629-639(1994).

Comments

CATALYTIC ACTIVITY: ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
ENZYME REGULATION: Feedback inhibition by lysine and threonine (By similarity).
PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 1/4 .
PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3 .
PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5 .
SUBUNIT: Tetramer consisting of 2 isoforms Alpha (catalytic) and 2 isoforms Beta (function not known) (By similarity).

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative initiation.

Name	Alpha
Synonyms	Aspartokinase subunit alpha
Isoform ID	P0A4Z9-1
This is the isoform sequence displayed in this entry.

Name	Beta
Synonyms	Aspartokinase subunit beta
Isoform ID	P0A4Z9-2
Note: No experimental confirmation available.
Features which should be applied to build the isoform sequence: VSP_018661.

SIMILARITY: Belongs to the aspartokinase family.
SIMILARITY: Contains 2 ACT domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BX248347; CAD95922.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
Z18290; CAA79160.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S42425; S42425.

RefSeq

NP_857374.1; -.

3D structure databases

ModBase

P0A4Z9.

Ontologies

GO:0016597;	Molecular function: amino acid binding (inferred from electronic annotation from InterPro).
GO:0004072;	Molecular function: aspartate kinase activity (inferred from electronic annotation from InterPro).
GO:0019877;	Biological process: diaminopimelate biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR002912; ACT_bd.
IPR001048; Asp/Glu/Uridylate_kinase.
IPR005260; Asp_kin_monofn.
IPR001341; Asp_kin_reg.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.1160.10; Aa_kinase; 1.

Pfam

PF00696; AA_kinase; 1.
PF01842; ACT; 2.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00656; asp_kin_monofn; 1.
TIGR00657; asp_kinases; 1.

PROSITE

PS00324; ASPARTOKINASE; 1.

Genome annotation databases

GeneID

1093392; -.

GenomeReviews

BX248333_GR; Mb3736c.

KEGG

mbo:Mb3736c; -.

Phylogenomic databases

HOGENOM

P0A4Z9; -.

Genome annotation databases

CMR

P0A4Z9; Mb3736c.

Other

ProtoNet

P0A4Z9.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative initiation; Amino-acid biosynthesis; Complete proteome; Diaminopimelate biosynthesis; Kinase; Lysine biosynthesis; Repeat; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 421`	`421`	`Aspartokinase.`	`PRO_0000002381`
`DOMAIN`	`266 338`	`73`	`ACT 1.`
`DOMAIN`	`348 413`	`66`	`ACT 2.`
`VAR_SEQ`	`1 249`		`Missing (in isoform Beta).`	`VSP_018661`

Sequence information

Length: 421 AA [This is the length of the unprocessed precursor]

Molecular weight: 44462 Da [This is the MW of the unprocessed precursor]

CRC64: 0567323B0D2085A7 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MALVVQKYGG SSVADAERIR RVAERIVATK KQGNDVVVVV SAMGDTTDDL LDLAQQVCPA 

        70         80         90        100        110        120 
PPPRELDMLL TAGERISNAL VAMAIESLGA HARSFTGSQA GVITTGTHGN AKIIDVTPGR 

       130        140        150        160        170        180 
LQTALEEGRV VLVAGFQGVS QDTKDVTTLG RGGSDTTAVA MAAALGADVC EIYTDVDGIF 

       190        200        210        220        230        240 
SADPRIVRNA RKLDTVTFEE MLEMAACGAK VLMLRCVEYA RRHNIPVHVR SSYSDRPGTV 

       250        260        270        280        290        300 
VVGSIKDVPM EDPILTGVAH DRSEAKVTIV GLPDIPGYAA KVFRAVADAD VNIDMVLQNV 

       310        320        330        340        350        360 
SKVEDGKTDI TFTCSRDVGP AAVEKLDSLR NEIGFSQLLY DDHIGKVSLI GAGMRSHPGV 

       370        380        390        400        410        420 
TATFCEALAA VGVNIELIST SEIRISVLCR DTELDKAVVA LHEAFGLGGD EEATVYAGTG 


R

P0A4Z9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools