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UniProtKB/Swiss-Prot entry P0A370


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CR1AB_BACTK
Primary accession number P0A370
Secondary accession numbers P06577 P06578 P09663 P09666 P09667 P21257 Q45789
Integrated into Swiss-Prot on January 1, 1988
Sequence was last modified on January 1, 1988 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 18)
Name and origin of the protein
Protein name Pesticidal crystal protein cry1Ab
Synonyms Insecticidal delta-endotoxin CryIA(b)
Crystaline entomocidal protoxin
130 kDa crystal protein
Gene name
Name: cry1Ab
Synonyms: bt2, cry-1-2, cry1A(b), cryIA(b), cryIC1
From
Bacillus thuringiensis subsp. kurstaki [TaxID: 29339] 
Encoded on Plasmid.
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus; Bacillus cereus group.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=HD-1-Dippel;
DOI=10.1016/0378-1119(86)90357-4; PubMed=3557124 [NCBI, ExPASy, EBI, Israel, Japan]
Geiser M., Schweitzer S., Grimm C.;
"The hypervariable region in the genes coding for entomopathogenic crystal proteins of Bacillus thuringiensis: nucleotide sequence of the kurhd1 gene of subsp. kurstaki HD1.";
Gene 48:109-118(1986).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=HD-1-Dippel;
PubMed=3011746 [NCBI, ExPASy, EBI, Israel, Japan]
Thorne L., Garduno F., Thompson T., Decker D., Zounes M., Wild M., Walfield A.M., Pollock T.J.;
"Structural similarity between the lepidoptera- and diptera-specific insecticidal endotoxin genes of Bacillus thuringiensis subsp. 'kurstaki' and 'israelensis'.";
J. Bacteriol. 166:801-811(1986).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=HD-1-Dippel;
AGRICOLA=IND87016057
Kondo S., Tamura N., Kunitate A., Hattori M., Akashi A., Ohmori I.;
"Cloning and nucleotide sequencing of two insecticidal delta-endotoxin genes from Bacillus thuringiensis var. kurstaki HD-1 DNA.";
Agric. Biol. Chem. 51:455-463(1987).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=HD-1-Dippel;
Fischhoff D.A., Bowdish K.S., Perlak F.J., Marrone P.G., McCormick S.M., Niedermeyer J.G., Dean D.A., Kusano-Kretzmer K., Mayer E.J., Rochester D.E., Rogers S.G., Fraley R.T.;
"Insect tolerant transgenic tomato plants.";
Biotechnology (N.Y.) 5:807-813(1987).
[5]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=NRD-12;
Hefford M.A., Brousseau R., Prefontaine G., Hanna Z., Condie J.A., Lau P.C.K.;
"Sequence of a lepidopteran toxin gene of Bacillus thuringiensis subsp kurstaki NRD-12.";
J. Biotechnol. 6:307-322(1987).
[6]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=S93;
Silva-Werneck J.O., De-Souza M.T., Dias J.M.C.S., Ribeiro B.M.;
"Characterization of Bacillus thuringiensis subsp. kurstaki strain S93 effective against the Fall armyworm, Spodoptera frugiperda and cloning of a cry1Ab gene.";
Can. J. Microbiol. 45:464-471(1999).
Comments
  • FUNCTION: Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.
  • DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.
  • BIOTECHNOLOGY: Introduced by genetic manipulation and expressed in insect-resistant maize by Monsanto, Northrup King and Ciba Geigy.
  • MISCELLANEOUS: Toxic segment of the protein is located in the N-terminus.
  • SIMILARITY: Belongs to the delta endotoxin family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M15271; AAA22561.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M12661; AAA22613.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
D00117; BAA00071.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M37263; AAA22420.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF059670; AAC64003.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
A03793; CAA00303.1; -; Unassigned_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
A09398; CAA00840.1; -; Unassigned_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A29125; A29125.
A29838; A29838.
A90025; JD0002.
I39838; I39838.
3D structure databases
HSSP P02965; 1CIY. [HSSP ENTRY / PDB]
SMR P0A370; 33-610.
ModBase P0A370.
Family and domain databases
InterPro IPR005638; Endotoxin_C.
IPR001178; Endotoxin_M.
IPR015790; Endotoxin_m_sub_1.
IPR005639; Endotoxin_N.
Graphical view of domain structure.
Gene3D G3DSA:2.100.10.10; Endotoxin_M; 1.
G3DSA:1.20.190.10; Endotoxin_N; 1.
Pfam PF03944; Endotoxin_C; 1.
PF00555; Endotoxin_M; 1.
PF03945; Endotoxin_N; 1.
Pfam graphical view of domain structure.
BLOCKS P0A370.
Other
ProtoNet P0A370.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Genetically modified food; Plasmid; Sporulation; Toxin.
Features
SEVIEWER logo Feature table viewer
KeyFrom    To Length Description FTId
CHAIN   1   1155  1155     Pesticidal crystal protein cry1Ab. PRO_0000174023
CONFLICT   207    207        A -> R (in Ref. 2; AAA22613). 
CONFLICT   382    406        VLDGTEFAYGTSSNLPSAVYRKSGT -> GPDGGRICLWNLLKFGQPPYTEKAEP (in Ref. 2; AAA22613). 
CONFLICT   410    410        L -> P (in Ref. 2; AAA22613). 
CONFLICT   430    432        LSH -> CLAY (in Ref. 2; AAA22613). 
CONFLICT   437    437        R -> Y (in Ref. 2; AAA22613). 
CONFLICT   447    447        I -> V (in Ref. 2; AAA22613). 
CONFLICT   452    458        MFSWIHR -> NDSSWTYC (in Ref. 2; AAA22613). 
CONFLICT   461    461        E -> N (in Ref. 5; AAA22420). 
CONFLICT   463    465        NNI -> GDV (in Ref. 2; AAA22613). 
CONFLICT   479    486        STNLGSGT -> LQSWLWN (in Ref. 2; AAA22613). 
CONFLICT   492    492        P -> L (in Ref. 2; AAA22613). 
CONFLICT   501    505        RRTSP -> EELT (in Ref. 2; AAA22613). 
CONFLICT   542    542        D -> H (in Ref. 5; AAA22420). 
CONFLICT   568    569        TV -> HL (in Ref. 5; AAA22420). 
CONFLICT   665    665        K -> E (in Ref. 5; AAA22420). 
CONFLICT   675    676        KR -> NG (in Ref. 5; AAA22420). 
CONFLICT   703    703        S -> N (in Ref. 2; AAA22613). 
CONFLICT   712    712        D -> H (in Ref. 2; AAA22613). 
CONFLICT   832    832        A -> E (in Ref. 4). 
CONFLICT   836    836        N -> I (in Ref. 5; AAA22420). 
CONFLICT   842    843        EK -> GRA (in Ref. 4). 
CONFLICT   1016   1016        E -> T (in Ref. 5; AAA22420). 
CONFLICT   1060   1060        E -> G (in Ref. 5; AAA22420). 
Sequence information
Length: 1155 AA [This is the length of the unprocessed precursor] Molecular weight: 130624 Da [This is the MW of the unprocessed precursor] CRC64: 43461A64C7AC7CAF [This is a checksum on the sequence]
        10         20         30         40         50         60 
MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF VPGAGFVLGL 

        70         80         90        100        110        120 
VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL EGLSNLYQIY AESFREWEAD 

       130        140        150        160        170        180 
PTNPALREEM RIQFNDMNSA LTTAIPLFAV QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ 

       190        200        210        220        230        240 
RWGFDAATIN SRYNDLTRLI GNYTDHAVRW YNTGLERVWG PDSRDWIRYN QFRRELTLTV 

       250        260        270        280        290        300 
LDIVSLFPNY DSRTYPIRTV SQLTREIYTN PVLENFDGSF RGSAQGIEGS IRSPHLMDIL 

       310        320        330        340        350        360 
NSITIYTDAH RGEYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI VAQLGQGVYR 

       370        380        390        400        410        420 
TLSSTLYRRP FNIGINNQQL SVLDGTEFAY GTSSNLPSAV YRKSGTVDSL DEIPPQNNNV 

       430        440        450        460        470        480 
PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA PMFSWIHRSA EFNNIIPSSQ ITQIPLTKST 

       490        500        510        520        530        540 
NLGSGTSVVK GPGFTGGDIL RRTSPGQIST LRVNITAPLS QRYRVRIRYA STTNLQFHTS 

       550        560        570        580        590        600 
IDGRPINQGN FSATMSSGSN LQSGSFRTVG FTTPFNFSNG SSVFTLSAHV FNSGNEVYID 

       610        620        630        640        650        660 
RIEFVPAEVT FEAEYDLERA QKAVNELFTS SNQIGLKTDV TDYHIDQVSN LVECLSDEFC 

       670        680        690        700        710        720 
LDEKKELSEK VKHAKRLSDE RNLLQDPNFR GINRQLDRGW RGSTDITIQG GDDVFKENYV 

       730        740        750        760        770        780 
TLLGTFDECY PTYLYQKIDE SKLKAYTRYQ LRGYIEDSQD LEIYLIRYNA KHETVNVPGT 

       790        800        810        820        830        840 
GSLWPLSAPS PIGKCAHHSH HFSLDIDVGC TDLNEDLGVW VIFKIKTQDG HARLGNLEFL 

       850        860        870        880        890        900 
EEKPLVGEAL ARVKRAEKKW RDKREKLEWE TNIVYKEAKE SVDALFVNSQ YDRLQADTNI 

       910        920        930        940        950        960 
AMIHAADKRV HSIREAYLPE LSVIPGVNAA IFEELEGRIF TAFSLYDARN VIKNGDFNNG 

       970        980        990       1000       1010       1020 
LSCWNVKGHV DVEEQNNHRS VLVVPEWEAE VSQEVRVCPG RGYILRVTAY KEGYGEGCVT 

      1030       1040       1050       1060       1070       1080 
IHEIENNTDE LKFSNCVEEE VYPNNTVTCN DYTATQEEYE GTYTSRNRGY DGAYESNSSV 

      1090       1100       1110       1120       1130       1140 
PADYASAYEE KAYTDGRRDN PCESNRGYGD YTPLPAGYVT KELEYFPETD KVWIEIGETE 

      1150 
GTFIVDSVEL LLMEE 

P0A370 in FASTA format

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