[1]	NUCLEOTIDE SEQUENCE [MRNA]. DOI=10.1093/nar/17.5.1893; PubMed=2928112 [NCBI, ExPASy, EBI, Israel, Japan] Sillekens P.T.G., Beijer R.P., Habets W.J., van Venrooij W.J.; "Molecular cloning of the cDNA for the human U2 snRNA-specific A' protein."; Nucleic Acids Res. 17:1893-1906(1989).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Muscle, and Urinary bladder; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[3]	PROTEIN SEQUENCE OF 2-20; 114-122; 133-143 AND 222-232, CLEAVAGE OF INITIATOR METHIONINE, AND MASS SPECTROMETRY. TISSUE=Colon carcinoma; Bienvenut W.V., Heiserich L., Gottlieb E.; Submitted (MAR-2008) to UniProtKB.
[4]	IDENTIFICATION IN A MRNA SPLICING COMPLEX WITH SFRS4; SFRS5; SNRNP70; SRRM1 AND SRRM2. PubMed=9531537 [NCBI, ExPASy, EBI, Israel, Japan] Blencowe B.J., Issner R., Nickerson J.A., Sharp P.A.; "A coactivator of pre-mRNA splicing."; Genes Dev. 12:996-1009(1998).
[5]	IDENTIFICATION IN A MRNA EXONIC SPLICING ENHANCER (ESE) COMPLEX WITH SNRNP70; SRRM1 AND TRA2B. DOI=10.1073/pnas.96.11.6125; PubMed=10339552 [NCBI, ExPASy, EBI, Israel, Japan] Eldridge A.G., Li Y., Sharp P.A., Blencowe B.J.; "The SRm160/300 splicing coactivator is required for exon-enhancer function."; Proc. Natl. Acad. Sci. U.S.A. 96:6125-6130(1999).
[6]	IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE SPICEOSOMAL C COMPLEX. DOI=10.1017/S1355838202021088; PubMed=11991638 [NCBI, ExPASy, EBI, Israel, Japan] Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.; "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."; RNA 8:426-439(2002).
[7]	PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-197, AND MASS SPECTROMETRY. TISSUE=Epithelium; DOI=10.1038/nbt1240; PubMed=16964243 [NCBI, ExPASy, EBI, Israel, Japan] Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.; "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."; Nat. Biotechnol. 24:1285-1292(2006).
[8]	PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-197, AND MASS SPECTROMETRY. DOI=10.1073/pnas.0805139105; PubMed=18669648 [NCBI, ExPASy, EBI, Israel, Japan] Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.; "A quantitative atlas of mitotic phosphorylation."; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
[9]	IDENTIFICATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY. Colinge J., Superti-Furga G., Bennett K.L.; Submitted (OCT-2008) to UniProtKB.
[10]	X-RAY CRYSTALLOGRAPHY (2.38 ANGSTROMS) OF 1-176. DOI=10.1038/29234; PubMed=9716128 [NCBI, ExPASy, EBI, Israel, Japan] Price S.R., Evans P.R., Nagai K.; "Crystal structure of the spliceosomal U2B'-U2A' protein complex bound to a fragment of U2 small nuclear RNA."; Nature 394:645-650(1998).

Comments

FUNCTION: This protein is associated with sn-RNP U2. It helps the A' protein to bind stem loop IV of U2 snRNA.
SUBUNIT: Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B.
INTERACTION:
P20823:HNF1A; NbExp=1; IntAct=EBI-876439, EBI-636034;
SUBCELLULAR LOCATION: Nucleus.
SIMILARITY: Belongs to the U2 small nuclear ribonucleoprotein A family.
SIMILARITY: Contains 3 LRR (leucine-rich) repeats.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X13482; CAA31838.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC022816; AAH22816.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC071717; AAH71717.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

IPI00297477; -.

S03616; S03616.

NP_003081.2; -.

3D structure databases

PDB

1A9N; X-ray; 2.38 A; A/C=1-176.

[ExPASy / RCSB / EBI]

PDBsum

1A9N; -.

ModBase

P09661.

Protein-protein interaction databases

DIP

DIP:625N; -.

IntAct

P09661; 11.

PTM databases

PhosphoSite

P09661; -.

Enzyme and pathway databases

Reactome

REACT_1675; mRNA Processing.
REACT_71; Gene Expression.

Organism-specific databases

GeneCards

GC15M099639; -.

H-InvDB

HIX0012630; -.
HIX0041327; -.

HGNC:11152; SNRPA1.

603521; gene. [NCBI / EBI]

PharmGKB

PA35994; -.

Gene expression databases

Bgee

P09661; -.

CleanEx

HS_SNRPA1; -.

GermOnline

ENSG00000131876; Homo sapiens.

Ontologies

GO:0005686;	Cellular component: snRNP U2 (traceable author statement from ProtInc).
GO:0005515;	Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0003723;	Molecular function: RNA binding (inferred from electronic annotation from UniProtKB-KW).
GO:0000398;	Biological process: nuclear mRNA splicing, via spliceosome (inferred by curator from HGNC).
QuickGo view.

Family and domain databases

InterPro

IPR003603; U2A'_phosphoprotein32A_C.
Graphical view of domain structure.

SMART

SM00446; LRRcap; 1.
SMART graphical view of domain structure.

Proteomic databases

PeptideAtlas

P09661; -.

PRIDE

P09661; -.

Genome annotation databases

Ensembl

ENSG00000131876; Homo sapiens. [Contig view]

GeneID

6627; -.

KEGG

hsa:6627; -.

Phylogenomic databases

HOGENOM

P09661; -.

HOVERGEN

P09661; -.

OMA

P09661; TADLIWK.

Other

NextBio

25813; -.

SOURCE

SNRPA1; Homo sapiens.

ProtoNet

P09661.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Direct protein sequencing; Leucine-rich repeat; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Polymorphism; Repeat; Ribonucleoprotein; RNA-binding; Spliceosome.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 255`	`254`	`U2 small nuclear ribonucleoprotein A'.`	`PRO_0000074173`
`REPEAT`	`41 63`	`23`	`LRR 1.`
`REPEAT`	`64 86`	`23`	`LRR 2.`
`REPEAT`	`88 111`	`24`	`LRR 3.`
`MOD_RES`	`197 197`		`Phosphoserine.`
`VARIANT`	`234 234`	`1`	`R -> H (in dbSNP:rs1050843 [NCBI]).`	`VAR_052030`
`CONFLICT`	`193 193`		`K -> R (in Ref. 1; CAA31838).`
`HELIX`	`6 10`	`5`
`STRAND`	`14 16`	`3`
`STRAND`	`22 25`	`4`
`HELIX`	`37 40`	`4`
`STRAND`	`45 48`	`4`
`STRAND`	`68 70`	`3`
`HELIX`	`83 86`	`4`
`STRAND`	`92 94`	`3`
`HELIX`	`103 111`	`9`
`STRAND`	`117 119`	`3`
`HELIX`	`124 127`	`4`
`HELIX`	`131 138`	`8`
`STRAND`	`143 145`	`3`
`HELIX`	`152 160`	`9`
`HELIX`	`165 172`	`8`

Sequence information

Length: 255 AA [This is the length of the unprocessed precursor]

Molecular weight: 28416 Da [This is the MW of the unprocessed precursor]

CRC64: 25902F07992B7209 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVKLTAELIE QAAQYTNAVR DRELDLRGYK IPVIENLGAT LDQFDAIDFS DNEIRKLDGF 

        70         80         90        100        110        120 
PLLRRLKTLL VNNNRICRIG EGLDQALPCL TELILTNNSL VELGDLDPLA SLKSLTYLSI 

       130        140        150        160        170        180 
LRNPVTNKKH YRLYVIYKVP QVRVLDFQKV KLKERQEAEK MFKGKRGAQL AKDIARRSKT 

       190        200        210        220        230        240 
FNPGAGLPTD KKKGGPSPGD VEAIKNAIAN ASTLAEVERL KGLLQSGQIP GRERRSGPTD 

       250 
DGEEEMEEDT VTNGS

P09661 in FASTA format

View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools