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UniProtKB/Swiss-Prot entry P09086


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PO2F2_HUMAN
Primary accession number P09086
Secondary accession numbers Q16648 Q7M4M8 Q9BRS4
Integrated into Swiss-Prot on November 1, 1988
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    June 16, 2009 (Entry version 117)
Name and origin of the protein
Protein name POU domain, class 2, transcription factor 2
Synonyms Octamer-binding transcription factor 2
Oct-2
OTF-2
Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2
Gene name
Name: POU2F2
Synonyms: OCT2, OTF2
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE [MRNA] OF 450-479 (ISOFORM 1).
PubMed=3265124 [NCBI, ExPASy, EBI, Israel, Japan]
Clerc R.G., Corcoran L.M., Lebowitz J.H., Baltimore D., Sharp P.A.;
"The B-cell-specific Oct-2 protein contains POU box- and homeo box-type domains.";
Genes Dev. 2:1570-1581(1988).
[2]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, AND TISSUE SPECIFICITY.
TISSUE=B-cell lymphoma;
DOI=10.1038/336551a0; PubMed=2904654 [NCBI, ExPASy, EBI, Israel, Japan]
Scheidereit C., Cromlish J.A., Gerster T., Kawakami K., Balmaceda C.-G., Currie R.A., Roder R.G.;
"A human lymphoid-specific transcription factor that activates immunoglobulin genes is a homoeobox protein.";
Nature 336:551-557(1988).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.;
"Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
TISSUE=Lymph;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[5]
NUCLEOTIDE SEQUENCE [MRNA] OF 2-479 (ISOFORM 1).
TISSUE=B-cell;
DOI=10.1038/336544a0; PubMed=2904653 [NCBI, ExPASy, EBI, Israel, Japan]
Mueller M.M., Ruppert S., Schaffner W., Matthias P.;
"A cloned octamer transcription factor stimulates transcription from lymphoid-specific promoters in non-B cells.";
Nature 336:544-551(1988).
[6]
NUCLEOTIDE SEQUENCE [MRNA] OF 99-161, AND FUNCTION.
PubMed=2328728 [NCBI, ExPASy, EBI, Israel, Japan]
Muller-Immergluck M.M., Schaffner W., Matthias P.;
"Transcription factor Oct-2A contains functionally redundant activating domains and works selectively from a promoter but not from a remote enhancer position in non-lymphoid (HeLa) cells.";
EMBO J. 9:1625-1634(1990).
[7]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 168-377.
PubMed=7888080 [NCBI, ExPASy, EBI, Israel, Japan]
Matsuo K., Clay O., Kuenzler P., Georgiev O., Urbanek P., Schaffner W.;
"Short introns interrupting the Oct-2 POU domain may prevent recombination between POU family genes without interfering with potential POU domain 'shuffling' in evolution.";
Biol. Chem. Hoppe-Seyler 375:675-683(1994).
[8]
NUCLEOTIDE SEQUENCE [MRNA] OF 240-387, AND FUNCTION.
DOI=10.1016/0092-8674(88)90016-5; PubMed=2901913 [NCBI, ExPASy, EBI, Israel, Japan]
Ko H.-S., Fast P., McBride W., Staudt L.M.;
"A human protein specific for the immunoglobulin octamer DNA motif contains a functional homeobox domain.";
Cell 55:135-144(1988).
[9]
NUCLEOTIDE SEQUENCE [MRNA] OF 468-479 (ISOFORM 5), AND FUNCTION.
DOI=10.1016/0092-8674(92)90150-B; PubMed=1739980 [NCBI, ExPASy, EBI, Israel, Japan]
Tanaka M., Lai J.-S., Herr W.;
"Promoter-selective activation domains in Oct-1 and Oct-2 direct differential activation of an snRNA and mRNA promoter.";
Cell 68:755-767(1992).
[10]
IDENTIFICATION (ISOFORM 5), AND TISSUE SPECIFICITY.
PubMed=3072196 [NCBI, ExPASy, EBI, Israel, Japan]
Schreiber E., Matthias P., Mueller M.M., Schaffner W.;
"Identification of a novel lymphoid specific octamer binding protein (OTF-2B) by proteolytic clipping bandshift assay (PCBA).";
EMBO J. 7:4221-4229(1988).
[11]
INTERACTION WITH NR3C1; AR AND PGR.
DOI=10.1074/jbc.274.38.26713; PubMed=10480874 [NCBI, ExPASy, EBI, Israel, Japan]
Prefontaine G.G., Walther R., Giffin W., Lemieux M.E., Pope L., Hache R.J.G.;
"Selective binding of steroid hormone receptors to octamer transcription factors determines transcriptional synergism at the mouse mammary tumor virus promoter.";
J. Biol. Chem. 274:26713-26719(1999).
[12]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-250, AND MASS SPECTROMETRY.
DOI=10.1073/pnas.0805139105; PubMed=18669648 [NCBI, ExPASy, EBI, Israel, Japan]
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.;
"A quantitative atlas of mitotic phosphorylation.";
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
[13]
STRUCTURE BY NMR OF 297-359.
DOI=10.1021/bi00199a005; PubMed=7914745 [NCBI, ExPASy, EBI, Israel, Japan]
Sivaraja M., Botfield M.C., Mueller M., Jancso A., Weiss M.A.;
"Solution structure of a POU-specific homeodomain: 3D-NMR studies of human B-cell transcription factor Oct-2.";
Biochemistry 33:9845-9855(1994).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M36653; AAA36389.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X53468; CAA37562.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X53469; CAA37565.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M36542; AAA36732.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X13810; CAA32040.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT007438; AAP36106.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC006101; AAH06101.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M36718; AAA59978.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X13809; CAA32039.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X81030; CAA56933.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00002666; -.
IPI00012533; -.
IPI00221055; -.
IPI00335926; -.
IPI00888702; -.
PIR A31753; A31753.
A42098; A42098.
RefSeq NP_002689.1; -.
UniGene Hs.654420
3D structure databases
PDB
1HDP; NMR; -; A=297-359.[ExPASy / RCSB / EBI]
PDBsum 1HDP; -.
SMR P09086; 199-357.
ModBase P09086.
Protein-protein interaction databases
DIP DIP:51N; -.
PTM databases
PhosphoSite P09086; -.
Enzyme and pathway databases
Pathway_Interaction_DB bcr_5pathway; BCR signaling pathway.
Organism-specific databases
GeneCards GC19M047286; -.
H-InvDB HIX0015168; -.
HGNC HGNC:9213; POU2F2.
GenAtlas POU2F2.
HPA CAB002513; -.
MIM 164176; gene. [NCBI / EBI]
PharmGKB PA33537; -.
Gene expression databases
ArrayExpress P09086; -.
Bgee P09086; -.
CleanEx HS_POU2F2; -.
GermOnline ENSG00000028277; Homo sapiens.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005634; Cellular component: nucleus (inferred from direct assay from MGI).
GO:0043565; Molecular function: sequence-specific DNA binding (inferred from direct assay from MGI).
GO:0003700; Molecular function: transcription factor activity (traceable author statement from ProtInc).
GO:0006959; Biological process: humoral immune response (traceable author statement from ProtInc).
GO:0006355; Biological process: regulation of transcription, DNA-dependent (inferred from electronic annotation from UniProtKB-KW).
GO:0006366; Biological process: transcription from RNA polymerase II promoter (traceable author statement from ProtInc).
QuickGo view.
Family and domain databases
InterPro IPR001356; Homeobox.
IPR017970; Homeobox_CS.
IPR012287; Homeodomain-rel.
IPR013847; POU.
IPR000327; POU_specific.
IPR000972; TF_octamer_bd.
Graphical view of domain structure.
Gene3D G3DSA:1.10.10.60; Homeodomain-rel; 1.
Pfam PF00046; Homeobox; 1.
PF00157; Pou; 1.
Pfam graphical view of domain structure.
PRINTS PR00029; OCTAMER.
PR00028; POUDOMAIN.
ProDom PD000010; Homeobox; 1.
PD000583; POU; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00389; HOX; 1.
SM00352; POU; 1.
SMART graphical view of domain structure.
PROSITE PS00027; HOMEOBOX_1; 1.
PS50071; HOMEOBOX_2; 1.
PS00035; POU_1; 1.
PS00465; POU_2; 1.
PS51179; POU_3; 1.
PROSITE graphical view of domain structure (profiles).
Proteomic databases
PRIDE P09086; -.
Genome annotation databases
Ensembl ENSG00000028277; Homo sapiens. [Contig view]
GeneID 5452; -.
KEGG hsa:5452; -.
Phylogenomic databases
HOGENOM P09086; -.
HOVERGEN P09086; -.
OMA P09086; LFLNHTG.
Other
NextBio 21101; -.
SOURCE POU2F2; Homo sapiens.
ProtoNet P09086.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Activator; Alternative splicing; Cytoplasm; DNA-binding; Homeobox; Nucleus; Phosphoprotein; Transcription; Transcription regulation.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   479  478     POU domain, class 2, transcription factor 2. PRO_0000100714
DOMAIN   195   269  75     POU-specific. 
DOMAIN   389   410  22     Leucine-zipper. 
DNA_BIND   297   356  60     Homeobox. 
COMPBIAS   417   424  8     Gly-rich. 
MOD_RES   250   250        Phosphoserine. 
VAR_SEQ   168   183        Missing (in isoform 3 and isoform 4). VSP_002324
VAR_SEQ   400   416        VTTLSSAVGTLHPSRTA -> AQTPALKAATRLSACQA (in isoform 4). VSP_007848
VAR_SEQ   417   479        Missing (in isoform 4). VSP_007849
VAR_SEQ   468   479        Missing (in isoform 2). VSP_002325
VAR_SEQ   468   479        PGLWWNPAPYQP -> STMVGLSSGLSPALMSNNPLATIQALASGGTLPLTSLDGS GNLVLGAAGAAPGSPSLVTSPLFLNHTGLPLLSAPPGVGL VSAAAAAVAASISSKSPGLSSSSSSSSSSSSSTCSDVAAQ TPGGPGGPEAGSKAE (in isoform 5). VSP_032187
MUTAGEN   340   342        VIR->FNP: Suppresses DNA-binding ability. 
HELIX   307   318  12      
HELIX   324   331  8      
TURN   332   335  4      
HELIX   338   350  13      
TURN   356   358  3      
Sequence information
Length: 479 AA [This is the length of the unprocessed precursor] Molecular weight: 51209 Da [This is the MW of the unprocessed precursor] CRC64: 8DA661DC07644D43 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MVHSSMGAPE IRMSKPLEAE KQGLDSPSEH TDTERNGPDT NHQNPQNKTS PFSVSPTGPS 

        70         80         90        100        110        120 
TKIKAEDPSG DSAPAAPLPP QPAQPHLPQA QLMLTGSQLA GDIQQLLQLQ QLVLVPGHHL 

       130        140        150        160        170        180 
QPPAQFLLPQ AQQSQPGLLP TPNLFQLPQQ TQGALLTSQP RAGLPTQAVT RPTLPDPHLS 

       190        200        210        220        230        240 
HPQPPKCLEP PSHPEEPSDL EELEQFARTF KQRRIKLGFT QGDVGLAMGK LYGNDFSQTT 

       250        260        270        280        290        300 
ISRFEALNLS FKNMCKLKPL LEKWLNDAET MSVDSSLPSP NQLSSPSLGF DGLPGRRRKK 

       310        320        330        340        350        360 
RTSIETNVRF ALEKSFLANQ KPTSEEILLI AEQLHMEKEV IRVWFCNRRQ KEKRINPCSA 

       370        380        390        400        410        420 
APMLPSPGKP ASYSPHMVTP QGGAGTLPLS QASSSLSTTV TTLSSAVGTL HPSRTAGGGG 

       430        440        450        460        470 
GGGGAAPPLN SIPSVTPPPP ATTNSTNPSP QGSHSAIGLS GLNPSTGPGL WWNPAPYQP 

P09086 in FASTA format

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