[1]	NUCLEOTIDE SEQUENCE [MRNA]. PubMed=3390159 [NCBI, ExPASy, EBI, Israel, Japan] Segil N., Shrutkowski A., Dworkin M.B., Dworkin-Rastl E.; "Enolase isoenzymes in adult and developing Xenopus laevis and characterization of a cloned enolase sequence."; Biochem. J. 251:31-39(1988).

Comments

CATALYTIC ACTIVITY: 2-phospho-D-glycerate = phosphoenolpyruvate + H₂O.
COFACTOR: Magnesium. Required for catalysis and for stabilizing the dimer.
PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5 .
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Cytoplasm.
SIMILARITY: Belongs to the enolase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

Y00718; CAA68706.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S00463; NOXL.

3D structure databases

HSSP

P56252; 1PDZ. [HSSP ENTRY / PDB]

SMR

P08734; 2-431.

ModBase

P08734.

Family and domain databases

InterPro

IPR000941; Enolase.
Graphical view of domain structure.

PANTHER

PTHR11902; Enolase; 1.

Pfam

PF00113; Enolase_C; 1.
PF03952; Enolase_N; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF001400; Enolase; 1.

PRINTS

PR00148; ENOLASE.

ProDom

PD000902; Enolase; 1.
[Domain structure / List of seq. sharing at least 1 domain]

TIGR01060; eno; 1.

PS00164; ENOLASE; 1.

Phylogenomic databases

HOVERGEN

P08734; -.

Other

ProtoNet

P08734.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; Glycolysis; Lyase; Magnesium; Metal-binding.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed (By similarity).`
`CHAIN`	`2 434`	`433`	`Alpha-enolase.`	`PRO_0000134106`
`REGION`	`370 373`	`4`	`Substrate binding (By similarity).`
`ACT_SITE`	`210 210`		`Proton donor (By similarity).`
`ACT_SITE`	`343 343`		`Proton acceptor (By similarity).`
`METAL`	`245 245`		`Magnesium (By similarity).`
`METAL`	`293 293`		`Magnesium (By similarity).`
`METAL`	`318 318`		`Magnesium (By similarity).`
`BINDING`	`158 158`		`Substrate (By similarity).`
`BINDING`	`167 167`		`Substrate (By similarity).`
`BINDING`	`293 293`		`Substrate (By similarity).`
`BINDING`	`318 318`		`Substrate (By similarity).`
`BINDING`	`394 394`		`Substrate (By similarity).`

Sequence information

Length: 434 AA [This is the length of the unprocessed precursor]

Molecular weight: 47504 Da [This is the MW of the unprocessed precursor]

CRC64: 9126C2F39477E3A4 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSIKNIRARE IFDSRGNPTV EVDLYTCKGL FRAAVPSGAS TGIYEALELR DNDKTRYLGK 

        70         80         90        100        110        120 
GVGRAVKYVN EFLGPALCTQ NLNVVEQEKI DKLMIEMDGT ENKSKFGANA LLGVSLAVCK 

       130        140        150        160        170        180 
AGAAEKGVPL YRHIADLAGN PEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGADSFKE 

       190        200        210        220        230        240 
AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEALELLKTA INKAGYPDKI 

       250        260        270        280        290        300 
VIGMDVAASE FYRDGKYDLD FKSPDDPSRY ISPDKLAELY MSFVKNYPVV SIEDPFDQDH 

       310        320        330        340        350        360 
WEAWTKFTAA SGIQVVGDDL TVTNPKRIAK AVEEKACNCL LLKVNQIGTV TESLEACKLA 

       370        380        390        400        410        420 
QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK 

       430 
ARFAGKNFRK PVFN

P08734 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools