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UniProtKB/Swiss-Prot entry P08478

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name AMD1_XENLA
Primary accession number P08478
Secondary accession numbers Q5D0B6 Q91697
Integrated into Swiss-Prot on August 1, 1988
Sequence was last modified on September 5, 2006 (Sequence version 2)
Annotations were last modified on    November 4, 2008 (Entry version 68)
Name and origin of the protein
Protein name Peptidyl-glycine alpha-amidating monooxygenase A [Precursor]
Synonyms PAM-A
Peptidyl-glycine alpha-amidating monooxygenase I
Peptide C-terminal alpha-amidating enzyme I
AE-I
Includes Peptidylglycine alpha-hydroxylating monooxygenase A
     (PHM-A)
     (EC 1.14.17.3)
Peptidyl-alpha-hydroxyglycine alpha-amidating lyase A
     (EC 4.3.2.5)
     (Peptidylamidoglycolate lyase-A)
     (PAL-A)
Gene name
Name: pam-A
From
Xenopus laevis (African clawed frog) [TaxID: 8355] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia; Batrachia; Anura; Mesobatrachia; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND PARTIAL PROTEIN SEQUENCE.
DOI=10.1016/0006-291X(87)90911-9; PubMed=3689360 [NCBI, ExPASy, EBI, Israel, Japan]
Mizuno K., Ohsuye K., Wada Y., Fuchimura K., Tanaka S., Matsuo H.;
"Cloning and sequence of cDNA encoding a peptide C-terminal alpha-amidating enzyme from Xenopus laevis.";
Biochem. Biophys. Res. Commun. 148:546-552(1987).
[2]
 NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE, AND FUNCTION.
TISSUE=Skin;
DOI=10.1111/j.1432-1033.1991.tb16314.x; PubMed=1935950 [NCBI, ExPASy, EBI, Israel, Japan]
Iwasaki Y., Kawahara T., Shimoi H., Suzuki K., Ghisalba O., Kangawa K., Matsuo H., Nishikawa Y.;
"Purification and cDNA cloning of Xenopus laevis skin peptidylhydroxyglycine N-C lyase, catalyzing the second reaction of C-terminal alpha-amidation.";
Eur. J. Biochem. 201:551-559(1991).
[3]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
TISSUE=Embryo;
NIH - Xenopus Gene Collection (XGC) project;
Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M18134; AAA49640.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X62771; CAA44615.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC043987; AAH43987.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A29726; URXLA1.
S17855; S17855.
RefSeq NP_001079520.2; -.
UniGene Xl.417
3D structure databases
HSSP P14925; 1PHM. [HSSP ENTRY / PDB]
ModBase P08478.
Organism-specific databases
Xenbase XB-FEAT-950934; pam.
Ontologies
GO
GO:0031410; Cellular component: cytoplasmic vesicle (inferred from electronic annotation from UniProtKB-KW).
GO:0016021; Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0004598; Molecular function: peptidylamidoglycolate lyase activity (inferred from electronic annotation from EC).
GO:0008270; Molecular function: zinc ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR011042; 6-blade_b-propeller_TolB-like.
IPR014783; Cu2_ascorb_mOase_C.
IPR014784; Cu2_ascorb_mOase_like_C.
IPR000323; Cu2_ascorb_mOase_N.
IPR001258; NHL_repeat.
IPR013017; NHL_repeat_subgr.
IPR000720; Pep_amidat_mOase.
Graphical view of domain structure.
Gene3D G3DSA:2.120.10.30; 6-blade_b-propeller_TolB-like; 1.
G3DSA:2.60.120.230; Cu2_ascorb_mOase_core; 1.
G3DSA:2.60.120.310; Cu2_ascorb_mOase_core; 1.
Pfam PF03712; Cu2_monoox_C; 1.
PF01082; Cu2_monooxygen; 1.
PF01436; NHL; 4.
Pfam graphical view of domain structure.
PRINTS PR00790; PAMONOXGNASE.
PROSITE PS00084; CU2_MONOOXYGENASE_1; 1.
PS00085; CU2_MONOOXYGENASE_2; 1.
PS51125; NHL; 4.
PROSITE graphical view of domain structure (profiles).
BLOCKS P08478.
ProtoNet P08478.
Genome annotation databases
GeneID 379207; -.
KEGG xla:379207; -.
Phylogenomic databases
HOVERGEN P08478; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; Copper; Cytoplasmic vesicle; Direct protein sequencing; Glycoprotein; Lyase; Membrane; Metal-binding; Monooxygenase; Multifunctional enzyme; Oxidoreductase; Repeat; Signal; Transmembrane; Zinc.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    36  36     Potential. 
CHAIN   37   935  899     Peptidyl-glycine alpha-amidating monooxygenase A. PRO_0000006367
TOPO_DOM   22   825  804     Intragranular (Potential). 
TRANSMEM   826   846  21     Potential. 
TOPO_DOM   847   935  89     Cytoplasmic (Potential). 
REPEAT   463   504  42     NHL 1. 
REPEAT   512   557  46     NHL 2. 
REPEAT   565   609  45     NHL 3. 
REPEAT   662   705  44     NHL 4. 
REGION   1   390  390     Peptidylglycine alpha-hydroxylating monooxygenase (By similarity). 
REGION   391   712  322     Peptidyl-alpha-hydroxyglycine alpha-amidating lyase (By similarity). 
METAL   103   103        Copper A (By similarity). 
METAL   104   104        Copper A (By similarity). 
METAL   168   168        Copper A (By similarity). 
METAL   238   238        Copper B (By similarity). 
METAL   240   240        Copper B (By similarity). 
METAL   310   310        Copper B (By similarity). 
CARBOHYD   658   658        N-linked (GlcNAc...) (Potential). 
CARBOHYD   739   739        N-linked (GlcNAc...) (Potential). 
DISULFID   43   182        By similarity. 
DISULFID   77   122        By similarity. 
DISULFID   110   127        By similarity. 
DISULFID   223   330        By similarity. 
DISULFID   289   311        By similarity. 
DISULFID   526   547        By similarity. 
DISULFID   594   605        By similarity. 
VAR_SEQ   391   400        DVHLEEDTDW -> GLITLGDSAV (in isoform 2). VSP_020312
VAR_SEQ   401   935        Missing (in isoform 2). VSP_020313
Sequence information
Length: 935 AA [This is the length of the unprocessed precursor] Molecular weight: 103379 Da [This is the MW of the unprocessed precursor] CRC64: 0463D2E57338D5AD [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MASLSSSFLV LFLLFQNSCY CFRSPLSVFK RYEESTRSLS NDCLGTTRPV MSPGSSDYTL 

        70         80         90        100        110        120 
DIRMPGVTPT ESDTYLCKSY RLPVDDEAYV VDFRPHANMD TAHHMLLFGC NIPSSTDDYW 

       130        140        150        160        170        180 
DCSAGTCMDK SSIMYAWAKN APPTKLPEGV GFRVGGKSGS RYFVLQVHYG NVKAFQDKHK 

       190        200        210        220        230        240 
DCTGVTVRVT PEKQPQIAGI YLSMSVDTVI PPGEEAVNSD IACLYNRPTI HPFAYRVHTH 

       250        260        270        280        290        300 
QLGQVVSGFR VRHGKWSLIG RQSPQLPQAF YPVEHPVEIS PGDIIATRCL FTGKGRTSAT 

       310        320        330        340        350        360 
YIGGTSNDEM CNLYIMYYMD AAHATSYMTC VQTGEPKLFQ NIPEIANVPI PVSPDMMMMM 

       370        380        390        400        410        420 
GHGHHHTEAE PEKNTGLQQP KREEEEVLDQ DVHLEEDTDW PGVNLKVGQV SGLALDPKNN 

       430        440        450        460        470        480 
LAIFHRGDHV WDENSFDRNF VYQQRGIGPI QESTILVVDP SSSKVLKSTG KNLFFLPHGL 

       490        500        510        520        530        540 
TIDRDGNYWV TDVALHQVFK LGAGKETPLL VLGRAFQPGS DRKHFCQPTD VAVDPITGNF 

       550        560        570        580        590        600 
FVADGYCNSR IMQFSPNGMF IMQWGEETSS NVPRPGQFRI PHSLTMVPDQ GQLCVADREN 

       610        620        630        640        650        660 
GRIQCFHAET GNFVKQIKHQ EFGREVFAVS YAPGGVLYAV NGKPYYGYSA PVQGFMLNFS 

       670        680        690        700        710        720 
NGDILDTFIP ARKNFDMPHD IAAADDGTVY VGDAHANAVW KFSPSKAEHR SVKKAGIEVE 

       730        740        750        760        770        780 
EITETEIFET HIRSRPKTNE SVEKQTQEKQ QKQKNSAGVS TQEKQNVVQE INAGVPTQEK 

       790        800        810        820        830        840 
QNVVQESSAG VSTQEKQSVV QESSAGVSTQ EKQSVVQESS AGVSFVLIIT LLIIPIAVLI 

       850        860        870        880        890        900 
AIAIFIRWRK VRMYGGDIDH KSESSSVGIL GKLRGKGSGG LNLGTFFATH KGYSRKGFDR 

       910        920        930 
LSTEGSDQEK DDDDGSDSEE EYSAPPIPPA PVSSS
P08478 in FASTA format

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