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UniProtKB/Swiss-Prot entry P08401


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CREC_ECOLI
Primary accession number P08401
Secondary accession number Q2M5R8
Integrated into Swiss-Prot on August 1, 1988
Sequence was last modified on July 19, 2003 (Sequence version 2)
Annotations were last modified on    November 4, 2008 (Entry version 93)
Name and origin of the protein
Protein name Sensor protein creC
Synonym EC 2.7.13.3
Gene name
Name: creC
Synonyms: phoM
OrderedLocusNames: b4399, JW4362
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=3531171 [NCBI, ExPASy, EBI, Israel, Japan]
Amemura M., Makino K., Shinagawa H., Nakata A.;
"Nucleotide sequence of the phoM region of Escherichia coli: four open reading frames may constitute an operon.";
J. Bacteriol. 168:294-302(1986).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1093/nar/23.12.2105; PubMed=7610040 [NCBI, ExPASy, EBI, Israel, Japan]
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes.";
Nucleic Acids Res. 23:2105-2119(1995).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[5]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 381-474.
STRAIN=K12;
PubMed=2835585 [NCBI, ExPASy, EBI, Israel, Japan]
Drury L.S., Buxton R.S.;
"Identification and sequencing of the Escherichia coli cet gene which codes for an inner membrane protein, mutation of which causes tolerance to colicin E2.";
Mol. Microbiol. 2:109-119(1988).
[6]
FUNCTION.
PubMed=2228961 [NCBI, ExPASy, EBI, Israel, Japan]
Amemura M., Makino K., Shinagawa H., Nakata A.;
"Cross talk to the phosphate regulon of Escherichia coli by PhoM protein: PhoM is a histidine protein kinase and catalyzes phosphorylation of PhoB and PhoM-open reading frame 2.";
J. Bacteriol. 172:6300-6307(1990).
[7]
TOPOLOGY [LARGE SCALE ANALYSIS].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.1109730; PubMed=15919996 [NCBI, ExPASy, EBI, Israel, Japan]
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
"Global topology analysis of the Escherichia coli inner membrane proteome.";
Science 308:1321-1323(2005).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M13608; AAA24375.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U14003; AAA97295.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC77352.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAE78388.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Y00538; CAA68601.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S56623; RGECFM.
RefSeq AP_004887.1; -.
NP_418816.1; -.
3D structure databases
ModBase P08401.
Protein-protein interaction databases
DIP DIP:9319N; -.
Enzyme and pathway databases
BioCyc EcoCyc:CREC-MON; -.
Organism-specific databases
EchoBASE EB0723; -.
EcoGene EG10730; creC.
Ontologies
GO
GO:0016021; Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005886; Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0000160; Biological process: two-component signal transduction system (phosphorelay) (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR003594; ATP_bd_ATPase.
IPR004358; Sig_transdc_His_kin-like_C.
IPR003661; Sig_transdc_His_kin_sub1_dim/P.
IPR005467; Sig_transdc_His_kinase_core.
IPR003660; Sig_transdc_His_kinase_HAMP.
Graphical view of domain structure.
Gene3D G3DSA:3.30.565.10; ATP_bd_ATPase; 1.
Pfam PF00672; HAMP; 1.
PF02518; HATPase_c; 1.
PF00512; HisKA; 1.
Pfam graphical view of domain structure.
PRINTS PR00344; BCTRLSENSOR.
SMART SM00304; HAMP; 1.
SM00387; HATPase_c; 1.
SM00388; HisKA; 1.
SMART graphical view of domain structure.
PROSITE PS50885; HAMP; 1.
PS50109; HIS_KIN; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P08401.
ProtoNet P08401.
Genome annotation databases
GeneID 948609; -.
GenomeReviews U00096_GR; b4399.
AP009048_GR; JW4362.
KEGG ecj:JW4362; -.
eco:b4399; -.
Phylogenomic databases
HOGENOM P08401; -.
Genome annotation databases
CMR P08401; b4399.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cell inner membrane; Cell membrane; Complete proteome; Kinase; Membrane; Phosphoprotein; Transferase; Transmembrane; Two-component regulatory system.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   474  474     Sensor protein creC. PRO_0000074742
TOPO_DOM   1     6  6     Periplasmic (Potential). 
TRANSMEM   7    27  21     Potential. 
TOPO_DOM   28   146  119     Cytoplasmic (Potential). 
TRANSMEM   147   167  21     Potential. 
TOPO_DOM   168   183  16     Periplasmic (Potential). 
TRANSMEM   184   204  21     Potential. 
TOPO_DOM   205   474  270     Cytoplasmic (Potential). 
DOMAIN   205   255  51     HAMP. 
DOMAIN   262   473  212     Histidine kinase. 
MOD_RES   265   265        Phosphohistidine; by autocatalysis (By similarity). 
CONFLICT   77    77        R -> P (in Ref. 1; AAA24375). 
Sequence information
Length: 474 AA [This is the length of the unprocessed precursor] Molecular weight: 52176 Da [This is the MW of the unprocessed precursor] CRC64: 9CEB8CCE406E3163 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MRIGMRLLLG YFLLVAVAAW FVLAIFVKEV KPGVRRATEG TLIDTATLLA ELARPDLLSG 

        70         80         90        100        110        120 
DPTHGQLAQA FNQLQHRPFR ANIGGINKVR NEYHVYMTDA QGKVLFDSAN KAVGQDYSRW 

       130        140        150        160        170        180 
NDVWLTLRGQ YGARSTLQNP ADPESSVMYV AAPIMDGSRL IGVLSVGKPN AAMAPVIKRS 

       190        200        210        220        230        240 
ERRILWASAI LLGIALVIGA GMVWWINRSI ARLTRYADSV TDNKPVPLPD LGSSELRKLA 

       250        260        270        280        290        300 
QALESMRVKL EGKNYIEQYV YALTHELKSP LAAIRGAAEI LREGPPPEVV ARFTDNILTQ 

       310        320        330        340        350        360 
NARMQALVET LLRQARLENR QEVVLTAVDV AALFRRVSEA RTVQLAEKKI TLHVTPTEVN 

       370        380        390        400        410        420 
VAAEPALLEQ ALGNLLDNAI DFTPESGCIT LSAEVDQEHV TLKVLDTGSG IPDYALSRIF 

       430        440        450        460        470 
ERFYSLPRAN GQKSSGLGLA FVSEVARLFN GEVTLRNVQE GGVLASLRLH RHFT 

P08401 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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