ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry P08289


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name PPBT_RAT
Primary accession number P08289
Secondary accession numbers P14055 P70707
Integrated into Swiss-Prot on August 1, 1988
Sequence was last modified on May 1, 1992 (Sequence version 2)
Annotations were last modified on    November 4, 2008 (Entry version 80)
Name and origin of the protein
Protein name Alkaline phosphatase, tissue-nonspecific isozyme [Precursor]
Synonyms EC 3.1.3.1
AP-TNAP
TNSALP
Alkaline phosphatase liver/bone/kidney isozyme
Gene name
Name: Alpl
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Placenta;
PubMed=3422431 [NCBI, ExPASy, EBI, Israel, Japan]
Thiede M.A., Yoon K., Golub E.E., Noda M., Rodan G.A.;
"Structure and expression of rat osteosarcoma (ROS 17/2.8) alkaline phosphatase: product of a single copy gene.";
Proc. Natl. Acad. Sci. U.S.A. 85:319-323(1988).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=Wistar;
TISSUE=Liver;
PubMed=2895632 [NCBI, ExPASy, EBI, Israel, Japan]
Misumi Y., Tashiro K., Hattori M., Sakaki Y., Ikehara Y.;
"Primary structure of rat liver alkaline phosphatase deduced from its cDNA.";
Biochem. J. 249:661-668(1988).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=Sprague-Dawley;
TISSUE=Liver;
PubMed=2544423 [NCBI, ExPASy, EBI, Israel, Japan]
Toh Y., Yamamoto M., Endo H., Misumi Y., Ikehara Y.;
"Isolation and characterization of a rat liver alkaline phosphatase gene. A single gene with two promoters.";
Eur. J. Biochem. 182:231-237(1989).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Kidney;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[5]
PROTEIN SEQUENCE OF 18-47, AND GPI-ANCHOR.
TISSUE=Liver;
PubMed=2834351 [NCBI, ExPASy, EBI, Israel, Japan]
Ogata S., Hayashi Y., Yasutake K., Ikehara Y.;
"Chemical identification of lipid components in the membranous form of rat liver alkaline phosphatase.";
J. Biochem. 102:1609-1615(1987).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Y00714; CAA68703.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
J03572; AAA41845.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X16028; CAA34160.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X16029; CAA34160.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X16030; CAA34160.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X16031; CAA34160.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X16032; CAA34160.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X16033; CAA34160.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X16034; CAA34160.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X16035; CAA34160.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X16036; CAA34160.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X16037; CAA34160.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X16038; CAA34160.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC088399; AAH88399.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A28114; A28114.
S00289; S00289.
RefSeq NP_037191.1; -.
UniGene Rn.82764
3D structure databases
HSSP P05187; 1EW2. [HSSP ENTRY / PDB]
ModBase P08289.
PTM databases
GlycoSuiteDB P08289; -.
Organism-specific databases
RGD 2100; Alpl.
Gene expression databases
ArrayExpress P08289; -.
GermOnline ENSRNOG00000013954; Rattus norvegicus.
Ontologies
GO
GO:0031225; Cellular component: anchored to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005886; Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0004035; Molecular function: alkaline phosphatase activity (inferred from electronic annotation from EC).
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008270; Molecular function: zinc ion binding (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR017849; Alkaline_Pase-like_a/b/a.
IPR001952; Alkaline_phosphatase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.720.10; Alk_phosphtse; 1.
Pfam PF00245; Alk_phosphatase; 1.
Pfam graphical view of domain structure.
PRINTS PR00113; ALKPHPHTASE.
SMART SM00098; alkPPc; 1.
SMART graphical view of domain structure.
PROSITE PS00123; ALKALINE_PHOSPHATASE; 1.
BLOCKS P08289.
ProtoNet P08289.
Genome annotation databases
Ensembl ENSRNOG00000013954; Rattus norvegicus. [Contig view]
GeneID 25586; -.
KEGG rno:25586; -.
NMPDR fig|10116.3.peg.24200; -.
Phylogenomic databases
HOVERGEN P08289; -.
Other
NextBio 607251; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cell membrane; Direct protein sequencing; Glycoprotein; GPI-anchor; Hydrolase; Lipoprotein; Magnesium; Membrane; Metal-binding; Phosphoprotein; Signal; Zinc.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    17  17      
CHAIN   18   501  484     Alkaline phosphatase, tissue-nonspecific isozyme. PRO_0000024027
PROPEP   502   524  23     Removed in mature form (Potential). PRO_0000024028
ACT_SITE   110   110        Phosphoserine intermediate. 
METAL   60    60        Magnesium (Potential). 
METAL   60    60        Zinc 2 (Potential). 
METAL   173   173        Magnesium (Potential). 
METAL   332   332        Magnesium (Potential). 
METAL   337   337        Zinc 1 (Potential). 
METAL   341   341        Zinc 1 (Potential). 
METAL   378   378        Zinc 2 (Potential). 
METAL   379   379        Zinc 2 (Potential). 
METAL   454   454        Zinc 1 (Potential). 
LIPID   501   501        GPI-anchor amidated serine (Potential). 
CARBOHYD   140   140        N-linked (GlcNAc...) (Potential). 
CARBOHYD   230   230        N-linked (GlcNAc...) (Potential). 
CARBOHYD   271   271        N-linked (GlcNAc...) (Potential). 
CARBOHYD   303   303        N-linked (GlcNAc...) (Potential). 
CARBOHYD   430   430        N-linked (GlcNAc...) (Potential). 
CONFLICT   12    12        T -> P (in Ref. 1; AAA41845). 
CONFLICT   116   116        A -> Y (in Ref. 2). 
CONFLICT   193   193        P -> R (in Ref. 1; AAA41845). 
CONFLICT   234   234        V -> E (in Ref. 2; CAA68703). 
CONFLICT   254   254        S -> T (in Ref. 2; CAA68703). 
CONFLICT   331   331        V -> E (in Ref. 2; CAA68703). 
CONFLICT   374   374        V -> L (in Ref. 2; CAA68703). 
CONFLICT   380   388        SHVFTFGGY -> HPTFSRLVA (in Ref. 2). 
CONFLICT   391   391        R -> Q (in Ref. 2). 
CONFLICT   463   463        A -> C (in Ref. 2; CAA68703). 
CONFLICT   474   474        V -> I (in Ref. 2; CAA68703). 
Sequence information
Length: 524 AA [This is the length of the unprocessed precursor] Molecular weight: 57659 Da [This is the MW of the unprocessed precursor] CRC64: BD75A4B87117DF03 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MILPFLVLAI GTCLTNSFVP EKEKDPSYWR QQAQETLKNA LKLQKLNTNV AKNIIMFLGD 

        70         80         90        100        110        120 
GMGVSTVTAA RILKGQLHHN TGEETRLEMD KFPFVALSKT YNTNAQVPDS AGTATAYLCG 

       130        140        150        160        170        180 
VKANEGTVGV SAATERTRCN TTQGNEVTSI LRWAKDAGKS VGIVTTTRVN HATPSAAYAH 

       190        200        210        220        230        240 
SADRDWYSDN EMPPEALSQG CKDIAYQLMH NIKDIDVIMG GGRKYMYPKN RTDVEYELDE 

       250        260        270        280        290        300 
KARGTRLDGL DLISIWKSFK PRHKHSHYVW NRTELLALDP SRVDYLLGLF EPGDMQYELN 

       310        320        330        340        350        360 
RNNLTDPSLS EMVEVALRIL TKNPKGFFLL VEGGRIDHGH HEGKAKQALH EAVEMDEAIG 

       370        380        390        400        410        420 
KAGTMTSQKD TLTVVTADHS HVFTFGGYTP RGNSIFGLAP MVSDTDKKPF TAILYGNGPG 

       430        440        450        460        470        480 
YKVVDGEREN VSMVDYAHNN YQAQSAVPLR HETHGGEDVA VFAKGPMAHL LHGVHEQNYI 

       490        500        510        520 
PHVMAYASCI GANLDHCAWA SSASSPSPGA LLLPLALFPL RTLF 

P08289 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by ch flag SIB Switzerland Mirror sites: Australia  Brazil  Canada  China  Korea
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!