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UniProtKB/Swiss-Prot entry P08266


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name RPB2_DROME
Primary accession number P08266
Secondary accession numbers Q04155 Q95027 Q9VFM7
Integrated into Swiss-Prot on August 1, 1988
Sequence was last modified on December 1, 2000 (Sequence version 2)
Annotations were last modified on    November 4, 2008 (Entry version 87)
Name and origin of the protein
Protein name DNA-directed RNA polymerase II subunit RPB2
Synonyms RNA polymerase II subunit B2
RNA polymerase II subunit 2
EC 2.7.7.6
DNA-directed RNA polymerase II 140 kDa polypeptide
Gene name
Name: RpII140
ORFNames: CG3180
From
Drosophila melanogaster (Fruit fly) [TaxID: 7227] 
Taxonomy Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
TISSUE=Embryo;
DOI=10.1016/0022-2836(87)90496-7; PubMed=3116266 [NCBI, ExPASy, EBI, Israel, Japan]
Falkenburg D., Dworniczak B., Faust D.M., Bautz E.K.F.;
"RNA polymerase II of Drosophila. Relation of its 140,000 Mr subunit to the beta subunit of Escherichia coli RNA polymerase.";
J. Mol. Biol. 195:929-937(1987).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Berkeley;
DOI=10.1126/science.287.5461.2185; PubMed=10731132 [NCBI, ExPASy, EBI, Israel, Japan]
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
"The genome sequence of Drosophila melanogaster.";
Science 287:2185-2195(2000).
[3]
GENOME REANNOTATION.
PubMed=12537572 [NCBI, ExPASy, EBI, Israel, Japan]
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.";
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=Berkeley;
TISSUE=Embryo;
PubMed=12537569 [NCBI, ExPASy, EBI, Israel, Japan]
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.;
"A Drosophila full-length cDNA resource.";
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
[5]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-69.
TISSUE=Embryo;
DOI=10.1016/0378-1119(91)90361-E; PubMed=1905256 [NCBI, ExPASy, EBI, Israel, Japan]
Sitzler S., Oldenburg I., Petersen G., Bautz E.K.F.;
"Analysis of the promoter region of the housekeeping gene DmRP140 by sequence comparison of Drosophila melanogaster and Drosophila virilis.";
Gene 100:155-162(1991).
Comments
  • FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).
  • CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
  • SUBUNIT: Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits (By similarity).
  • SUBCELLULAR LOCATION: Nucleus (By similarity).
  • MISCELLANEOUS: The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits (By similarity).
  • SIMILARITY: Belongs to the RNA polymerase beta chain family.
  • SEQUENCE CAUTION:
    • Sequence=CAA29180.2; Type=Frameshift; Positions=43; Note=The frameshift leads to an erroneous gene model prediction
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X05709; CAA29180.2; ALT_FRAME; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE014297; AAF55024.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT003265; AAO25022.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M62972; AAA28476.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A27826; A27826.
RefSeq NP_476706.1; -.
UniGene Dm.6926
3D structure databases
HSSP P08518; 1I50. [HSSP ENTRY / PDB]
ModBase P08266.
Protein-protein interaction databases
DIP DIP:17498N; -.
IntAct P08266; -.
Enzyme and pathway databases
BioCyc DMEL-XXX-02:DMEL-XXX-02-011546-MON; -.
Organism-specific databases
FlyBase FBgn0003276; RpII140.
Gene expression databases
ArrayExpress P08266; -.
GermOnline CG3180; Drosophila melanogaster.
Ontologies
GO
GO:0005665; Cellular component: DNA-directed RNA polymerase II, core complex (non-traceable author statement from UniProtKB).
GO:0003899; Molecular function: DNA-directed RNA polymerase activity (non-traceable author statement from UniProtKB).
GO:0006366; Biological process: transcription from RNA polymerase II promoter (non-traceable author statement from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR015712; DNA-dir_RNA_pol_su2.
IPR007120; DNA-dir_RNA_pol_su2_6.
IPR007121; RNA_pol_bsu_CS.
IPR007644; RNA_pol_bsu_protrusion.
IPR007642; RNA_pol_Rpb2_2.
IPR007645; RNA_pol_Rpb2_3.
IPR007646; RNA_pol_Rpb2_4.
IPR007647; RNA_pol_Rpb2_5.
IPR007641; RNA_pol_Rpb2_7.
Graphical view of domain structure.
PANTHER PTHR20856; RNA_pol_I_sub2; 1.
Pfam PF04563; RNA_pol_Rpb2_1; 1.
PF04561; RNA_pol_Rpb2_2; 1.
PF04565; RNA_pol_Rpb2_3; 1.
PF04566; RNA_pol_Rpb2_4; 1.
PF04567; RNA_pol_Rpb2_5; 1.
PF00562; RNA_pol_Rpb2_6; 1.
PF04560; RNA_pol_Rpb2_7; 1.
Pfam graphical view of domain structure.
PROSITE PS01166; RNA_POL_BETA; 1.
BLOCKS P08266.
ProtoNet P08266.
Genome annotation databases
Ensembl CG3180; Drosophila melanogaster. [Contig view]
GeneID 41721; -.
KEGG dme:Dmel_CG3180; -.
NMPDR fig|7227.3.peg.12716; -.
Phylogenomic databases
HOGENOM P08266; -.
Other
NextBio 825231; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; DNA-directed RNA polymerase; Magnesium; Metal-binding; Nucleotidyltransferase; Nucleus; Transcription; Transferase; Zinc; Zinc-finger.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   1176  1176     DNA-directed RNA polymerase II subunit RPB2. PRO_0000048084
ZN_FING   1121   1142  22     C4-type (By similarity). 
METAL   794    794        Magnesium; shared with RPB1 (By similarity). 
METAL   1121   1121        Zinc (By similarity). 
METAL   1124   1124        Zinc (By similarity). 
METAL   1139   1139        Zinc (By similarity). 
METAL   1142   1142        Zinc (By similarity). 
CONFLICT   72     72        A -> R (in Ref. 1; CAA29180). 
CONFLICT   666    667        ID -> MY (in Ref. 1; CAA29180). 
Sequence information
Length: 1176 AA [This is the length of the unprocessed precursor] Molecular weight: 134043 Da [This is the MW of the unprocessed precursor] CRC64: 224821B335BED7F0 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MMYDNEEELY EEENAEEISH ELWQEACWIV INAYFDEKGL VRQQLDSFDE FIQMSVQRIV 

        70         80         90        100        110        120 
EDSPAIELQA EAQHTSGEVE TPPRFSLKFE QIYLSKPTHW EKDGSPSPMM PNEARLRNLT 

       130        140        150        160        170        180 
YSAPLYVDIT KTKNVEGLDP VETQHQKTFI GKIPIMLRST YCLLSQLTDR DLTELNECPL 

       190        200        210        220        230        240 
DPGGYFIING SEKVLIAQEK MATNTVYVFS MKDGKYAFKT EIRSCLEHSS RPTSTLWVNM 

       250        260        270        280        290        300 
MARGSQNIKK SAIGQRIIAI LPYIKQEIPI MIVFRALGFV ADRDILEHII YDFDDPEMME 

       310        320        330        340        350        360 
MVKPSLDEAF VVQEQNVALN FIGARGARPG VTKDKRIKYA KEILQKEMLP HVGVSDFCET 

       370        380        390        400        410        420 
KKAYFLGYMV HRLLLASLGR RELDDRDHYG NKRLDLAGPL LAFLFRGLFK NLMKEVRMYT 

       430        440        450        460        470        480 
QKFIDRGKDF NLELAIKTNI ITDGLRYSLA TGNWGDQKKA HQARAGVSQV LNRLTFASTL 

       490        500        510        520        530        540 
SHLRRVNSPI GRDGKLAKPR QLHNTLWGML CPAETPEGAA VGLVKNLALM AYISVGSQPS 

       550        560        570        580        590        600 
PILEFLEEWS MENLEEIAPS AIADATKIFV NGCWVGIHRD PEQLMATLRK LRRQMDIIVS 

       610        620        630        640        650        660 
EVSMIRDIRD REIRIYTDAG RICRPLLIVE NGSLLLKKTH VEMLKERDYN NYSWQVLVAS 

       670        680        690        700        710        720 
GVVEYIDTLE EETVMIAMSP YDLKQDKDYA YCTTYTHCEI HPAMILGVCA SIIPFPDHNQ 

       730        740        750        760        770        780 
SPRNTYQSAM GKQAMGVYIT NFHVRMDTLA HVLYYPMKPL VTTRSMEYLR FRELPAGINS 

       790        800        810        820        830        840 
IVAILCYTGY NQEDSVILNA SAVERGFFRS VFYRSYKDSE NKRVGDQEEN FEKPHRGTCQ 

       850        860        870        880        890        900 
GMRNAHYDKL DDDGIIAPGI RVSGDDVVIG KTITLPENDD ELDSNTKRFS KRDASTFLRN 

       910        920        930        940        950        960 
SETGIVDQVM LTLNSEGYKF CKIRVRSVRI PQIGDKFASR HGQKGTCGIQ YRQEDMAFTC 

       970        980        990       1000       1010       1020 
EGLAPDIIIN PHAIPSRMTI GHLIECLQGK LGSNKGEIGD ATPFNDAVNV QKISTFLQEY 

      1030       1040       1050       1060       1070       1080 
GYHLRGNEVM YNGHTGRKIN AQVFLGPTYY QRLKHMVDDK IHSRARGPVQ ILVRQPMEGR 

      1090       1100       1110       1120       1130       1140 
ARDGGLRFGE MERDCQISHG AAQFLRERLF EVSDPYRVHI CNFCGLIAIA NLRNNTFECK 

      1150       1160       1170 
GCKNKTQISQ VRLPYAAKLL FQELMSMNIA PRLMVT 

P08266 in FASTA format

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