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UniProtKB/Swiss-Prot entry P08166


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name KAD2_BOVIN
Primary accession number P08166
Secondary accession numbers P08167 Q2KIJ7
Integrated into Swiss-Prot on August 1, 1988
Sequence was last modified on January 23, 2007 (Sequence version 2)
Annotations were last modified on    November 4, 2008 (Entry version 86)
Name and origin of the protein
Protein name Adenylate kinase isoenzyme 2, mitochondrial
Synonyms AK 2
EC 2.7.4.3
ATP-AMP transphosphorylase 2
Gene name
Name: AK2
From
Bos taurus (Bovine) [TaxID: 9913] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS AK2A AND AK2B).
PubMed=3040716 [NCBI, ExPASy, EBI, Israel, Japan]
Kishi F., Tanizawa Y., Nakazawa A.;
"Isolation and characterization of two types of cDNA for mitochondrial adenylate kinase and their expression in Escherichia coli.";
J. Biol. Chem. 262:11785-11789(1987).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0378-1119(90)90228-J; PubMed=2227435 [NCBI, ExPASy, EBI, Israel, Japan]
Tanaka H., Yamada M., Kishi F., Nakazawa A.;
"Isolation and characterization of the gene encoding bovine adenylate kinase isozyme 2.";
Gene 93:221-227(1990).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM AK2B).
DOI=10.1186/1471-2164-6-166; PubMed=16305752 [NCBI, ExPASy, EBI, Israel, Japan]
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
"Characterization of 954 bovine full-CDS cDNA sequences.";
BMC Genomics 6:166-166(2005).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM AK2B).
STRAIN=Hereford;
TISSUE=Testis;
NIH - Mammalian Gene Collection (MGC) project;
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
[5]
PROTEIN SEQUENCE OF 2-239.
PubMed=3002789 [NCBI, ExPASy, EBI, Israel, Japan]
Frank R., Trosin M., Tomasselli A.G., Noda L., Krauth-Siegel R.L., Schirmer R.H.;
"Mitochondrial adenylate kinase (AK2) from bovine heart. The complete primary structure.";
Eur. J. Biochem. 154:205-211(1986).
[6]
X-RAY CRYSTALLOGRAPHY (1.92 ANGSTROMS).
PubMed=8868479 [NCBI, ExPASy, EBI, Israel, Japan]
Schlauderer G.J., Schulz G.E.;
"The structure of bovine mitochondrial adenylate kinase: comparison with isoenzymes in other compartments.";
Protein Sci. 5:434-441(1996).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M16224; AAA30364.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M16225; AAA30365.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
D90069; BAA14110.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
D90069; BAA14109.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT025476; ABF57432.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC112613; AAI12614.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR B29792; B29792.
JS0422; JS0422.
RefSeq NP_776314.1; -.
UniGene Bt.946
3D structure databases
PDB
1AK2; X-ray; 1.92 A; A=1-233.[ExPASy / RCSB / EBI]
2AK2; X-ray; 2.10 A; A=1-233.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1AK2; -.
2AK2; -.
ModBase P08166.
Ontologies
GO
GO:0005743; Cellular component: mitochondrial inner membrane (inferred from sequence or structural similarity from AgBase).
QuickGo view.
Family and domain databases
InterPro IPR006259; Adenyl_kin_sub.
IPR000850; Adenylate_kin.
IPR007862; Adenylate_kinase_Znf_lid.
Graphical view of domain structure.
PANTHER PTHR23359; Adenylate_kin; 1.
Pfam PF00406; ADK; 1.
PF05191; ADK_lid; 1.
Pfam graphical view of domain structure.
PRINTS PR00094; ADENYLTKNASE.
ProDom PD000657; Adenylate_kin; 1.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR01351; adk; 1.
PROSITE PS00113; ADENYLATE_KINASE; 1.
BLOCKS P08166.
ProtoNet P08166.
Genome annotation databases
Ensembl ENSBTAG00000017605; Bos taurus. [Contig view]
GeneID 280716; -.
KEGG bta:280716; -.
Phylogenomic databases
HOVERGEN P08166; -.
Other
LinkHub P08166; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Acetylation; Alternative splicing; ATP-binding; Direct protein sequencing; Kinase; Mitochondrion; Nucleotide-binding; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   241  240     Adenylate kinase isoenzyme 2, mitochondrial. PRO_0000158916
NP_BIND   24    32  9     ATP (By similarity). 
MOD_RES   95    95        N6-acetyllysine (By similarity). 
DISULFID   44    94         
VAR_SEQ   234   241        CKDLVMFI -> S (in isoform AK2B). VSP_002789
STRAND   19    23  5      
HELIX   30    41  12      
STRAND   44    47  4      
HELIX   48    58  11      
HELIX   61    71  11      
HELIX   78    89  12      
HELIX   92    94  3      
STRAND   98   102  5      
HELIX   107   120  14      
STRAND   126   131  6      
HELIX   134   142  9      
TURN   148   150  3      
STRAND   153   155  3      
TURN   156   158  3      
TURN   168   170  3      
HELIX   182   205  24      
STRAND   209   213  5      
HELIX   218   232  15      
Sequence information
Length: 241 AA [This is the length of the unprocessed precursor] Molecular weight: 26497 Da [This is the MW of the unprocessed precursor] CRC64: D9232C61C431F9F4 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAPNVPAAEP VPESPKGVRA VLLGPPGAGK GTQAPKLAKN FCVCHLATGD MLRAMVASGS 

        70         80         90        100        110        120 
ELGKKLKATM DAGKLVSDEM VLELIEKNLE TPPCKNGFLL DGFPRTVRQA EMLDDLMEKR 

       130        140        150        160        170        180 
KEKLDSVIEF SIPDSLLIRR ITGRLIHPQS GRSYHEEFNP PKEPMKDDIT GEPLIRRSDD 

       190        200        210        220        230        240 
NKKALKIRLE AYHTQTTPLV EYYSKRGIHS AIDASQTPDV VFASILAAFS KATCKDLVMF 


I 

P08166 in FASTA format

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