ID   DHSB_BACSU              Reviewed;         253 AA.
AC   P08066;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   04-NOV-2008, entry version 83.
DE   RecName: Full=Succinate dehydrogenase iron-sulfur subunit;
DE            EC=1.3.99.1;
GN   Name=sdhB; OrderedLocusNames=BSU28430;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / PY79;
RX   MEDLINE=87109084; PubMed=3027051;
RA   Phillips M.K., Hederstedt L., Hasnain S., Rutberg L., Guest J.R.;
RT   "Nucleotide sequence encoding the flavoprotein and iron-sulfur protein
RT   subunits of the Bacillus subtilis PY79 succinate dehydrogenase
RT   complex.";
RL   J. Bacteriol. 169:864-873(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=97124191; PubMed=8969504;
RA   Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J.,
RA   Emmerson P.T., Harwood C.R.;
RT   "The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus
RT   subtilis chromosome containing genes responsible for stress responses,
RT   the utilization of plant cell walls and primary metabolism.";
RL   Microbiology 142:3067-3078(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 112-253.
RX   MEDLINE=87310370; PubMed=3114423;
RA   Cutting S.M., Mandelstam J.;
RT   "The nucleotide sequence and the transcription during sporulation of
RT   the gerE gene of Bacillus subtilis.";
RL   J. Gen. Microbiol. 132:3013-3024(1986).
CC   -!- CATALYTIC ACTIVITY: Succinate + acceptor = fumarate + reduced
CC       acceptor.
CC   -!- COFACTOR: Binds 1 2Fe-2S cluster.
CC   -!- COFACTOR: Binds 1 3Fe-4S cluster.
CC   -!- COFACTOR: Binds 1 4Fe-4S cluster.
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle.
CC   -!- SUBUNIT: In B.subtilis succinate dehydrogenase forms part of an
CC       enzyme complex containing three subunits: a flavoprotein, an iron-
CC       sulfur protein and cytochrome b-558.
CC   -!- SIMILARITY: Belongs to the succinate dehydrogenase/fumarate
CC       reductase iron-sulfur protein family.
CC   -!- SIMILARITY: Contains 1 4Fe-4S ferredoxin-type domain.
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DR   EMBL; M13470; AAA22747.1; -; Genomic_DNA.
DR   EMBL; Z75208; CAA99548.1; -; Genomic_DNA.
DR   EMBL; Z99118; CAB14803.1; -; Genomic_DNA.
DR   EMBL; M17642; AAA22471.1; -; Genomic_DNA.
DR   PIR; B27763; B27763.
DR   RefSeq; NP_390721.1; -.
DR   HSSP; P17596; 1QLA.
DR   GeneID; 937460; -.
DR   GenomeReviews; AL009126_GR; BSU28430.
DR   KEGG; bsu:BSU28430; -.
DR   NMPDR; fig|224308.1.peg.2846; -.
DR   SubtiList; BG10353; sdhB.
DR   HOGENOM; P08066; -.
DR   BioCyc; BSUB224308:BSU2839-MON; -.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000104; F:succinate dehydrogenase activity; IEA:EC.
DR   GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
DR   GO; GO:0006810; P:transport; IEA:UniProtKB-KW.
DR   InterPro; IPR001450; 4Fe4S_Fe_S_bd.
DR   InterPro; IPR012675; b-grasp_ferredoxin-like.
DR   InterPro; IPR012285; Fum_reductase_C.
DR   InterPro; IPR004489; Succ_DHase/fum_Rdtase_Fe-S.
DR   Gene3D; G3DSA:3.10.20.30; Ferredoxin_fold; 1.
DR   Gene3D; G3DSA:1.10.1060.10; Fum_reductase_C; 1.
DR   Pfam; PF00037; Fer4; 1.
DR   TIGRFAMs; TIGR00384; dhsB; 1.
DR   PROSITE; PS00198; 4FE4S_FER_1; 1.
DR   PROSITE; PS51379; 4FE4S_FER_2; 1.
PE   3: Inferred from homology;
KW   2Fe-2S; 3Fe-4S; 4Fe-4S; Complete proteome; Electron transport; Iron;
KW   Iron-sulfur; Metal-binding; Oxidoreductase; Transport;
KW   Tricarboxylic acid cycle.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    253       Succinate dehydrogenase iron-sulfur
FT                                subunit.
FT                                /FTId=PRO_0000158686.
FT   DOMAIN      146    174       4Fe-4S ferredoxin-type.
FT   METAL        64     64       Iron-sulfur 1 (2Fe-2S) (By similarity).
FT   METAL        69     69       Iron-sulfur 1 (2Fe-2S) (By similarity).
FT   METAL        72     72       Iron-sulfur 1 (2Fe-2S) (By similarity).
FT   METAL        84     84       Iron-sulfur 1 (2Fe-2S) (By similarity).
FT   METAL       155    155       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       158    158       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       161    161       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       165    165       Iron-sulfur 3 (3Fe-4S) (By similarity).
FT   METAL       212    212       Iron-sulfur 3 (3Fe-4S) (By similarity).
FT   METAL       218    218       Iron-sulfur 3 (3Fe-4S) (By similarity).
FT   METAL       222    222       Iron-sulfur 2 (4Fe-4S) (By similarity).
SQ   SEQUENCE   253 AA;  28418 MW;  C20E095CDD960B3E CRC64;
     MSEQKTIRFI ITRQDTADST PYDEEFEIPY RPNLNVISAL MEIRRNPVNV KGEKTTPVTW
     DMNCLEEVCG ACSMVINGKP RQSCTALIDQ LEQPIRLKPM KTFPVVRDLQ VDRSRMFDSL
     KKVKAWIPID GTYDLGPGPR MPEKRRQWAY ELSKCMTCGV CLEACPNVNS KSKFMGPAPM
     SQVRLFNAHP TGAMNKSERL EALMDEGGLA DCGNSQNCVQ SCPKGIPLTT SIAALNRDTN
     LQAFRNFFGS DRV
//