ID   DHSA_BACSU              Reviewed;         586 AA.
AC   P08065;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   04-NOV-2008, entry version 81.
DE   RecName: Full=Succinate dehydrogenase flavoprotein subunit;
DE            EC=1.3.99.1;
GN   Name=sdhA; Synonyms=citF; OrderedLocusNames=BSU28440;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / PY79;
RX   MEDLINE=87109084; PubMed=3027051;
RA   Phillips M.K., Hederstedt L., Hasnain S., Rutberg L., Guest J.R.;
RT   "Nucleotide sequence encoding the flavoprotein and iron-sulfur protein
RT   subunits of the Bacillus subtilis PY79 succinate dehydrogenase
RT   complex.";
RL   J. Bacteriol. 169:864-873(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=97124191; PubMed=8969504;
RA   Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J.,
RA   Emmerson P.T., Harwood C.R.;
RT   "The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus
RT   subtilis chromosome containing genes responsible for stress responses,
RT   the utilization of plant cell walls and primary metabolism.";
RL   Microbiology 142:3067-3078(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31.
RC   STRAIN=168 / PY79;
RX   MEDLINE=86223767; PubMed=3086287;
RA   Magnusson K., Philips M.K., Guest J.R., Rutberg L.;
RT   "Nucleotide sequence of the gene for cytochrome b558 of the Bacillus
RT   subtilis succinate dehydrogenase complex.";
RL   J. Bacteriol. 166:1067-1071(1986).
RN   [5]
RP   MUTANT ASP-48.
RX   MEDLINE=87026613; PubMed=3021212; DOI=10.1021/bi00366a033;
RA   Maguire J.J., Magnusson K., Hederstedt L.;
RT   "Bacillus subtilis mutant succinate dehydrogenase lacking covalently
RT   bound flavin: identification of the primary defect and studies on the
RT   iron-sulfur clusters in mutated and wild-type enzyme.";
RL   Biochemistry 25:5202-5208(1986).
CC   -!- CATALYTIC ACTIVITY: Succinate + acceptor = fumarate + reduced
CC       acceptor.
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle.
CC   -!- SUBUNIT: In B.subtilis succinate dehydrogenase forms part of an
CC       enzyme complex containing three subunits: a flavoprotein, an iron-
CC       sulfur protein and cytochrome b-558.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein;
CC       Cytoplasmic side (By similarity).
CC   -!- SIMILARITY: Belongs to the FAD-dependent oxidoreductase 2 family.
CC       FRD/SDH subfamily.
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DR   EMBL; M13470; AAA22746.1; -; Genomic_DNA.
DR   EMBL; Z75208; CAA99547.1; -; Genomic_DNA.
DR   EMBL; Z99118; CAB14804.1; -; Genomic_DNA.
DR   PIR; A27763; A27763.
DR   RefSeq; NP_390722.1; -.
DR   HSSP; P83223; 1D4D.
DR   GeneID; 937600; -.
DR   GenomeReviews; AL009126_GR; BSU28440.
DR   KEGG; bsu:BSU28440; -.
DR   NMPDR; fig|224308.1.peg.2847; -.
DR   SubtiList; BG10352; sdhA.
DR   HOGENOM; P08065; -.
DR   BioCyc; BSUB224308:BSU2840-MON; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0000104; F:succinate dehydrogenase activity; IEA:EC.
DR   GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
DR   GO; GO:0006810; P:transport; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR003953; FAD_bind2_N.
DR   InterPro; IPR013027; FAD_pyr_nucl-diS_OxRdtase.
DR   InterPro; IPR003952; FRD_SDH_FAD_BS.
DR   InterPro; IPR004112; Fum_Rdtase/Succ_DHase_flav_C.
DR   InterPro; IPR001100; Pyr_nuc-diS_OxRdtase.
DR   InterPro; IPR011280; Succ_DHase/Fum_Rdtase_flav_su.
DR   PANTHER; PTHR11632:SF4; SDH_A_Bsu; 1.
DR   Pfam; PF00890; FAD_binding_2; 1.
DR   Pfam; PF02910; Succ_DH_flav_C; 1.
DR   PRINTS; PR00368; FADPNR.
DR   PRINTS; PR00411; PNDRDTASEI.
DR   TIGRFAMs; TIGR01811; sdhA_Bsu; 1.
DR   PROSITE; PS00504; FRD_SDH_FAD_BINDING; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Complete proteome; Electron transport; FAD;
KW   Flavoprotein; Membrane; Oxidoreductase; Transport;
KW   Tricarboxylic acid cycle.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    586       Succinate dehydrogenase flavoprotein
FT                                subunit.
FT                                /FTId=PRO_0000158650.
FT   NP_BIND      33     48       FAD (By similarity).
FT   ACT_SITE    285    285       Proton acceptor (By similarity).
FT   BINDING     236    236       Substrate (By similarity).
FT   BINDING     250    250       Substrate (By similarity).
FT   BINDING     352    352       Substrate (By similarity).
FT   BINDING     386    386       Substrate (By similarity).
FT   MOD_RES      41     41       Tele-8alpha-FAD histidine (By
FT                                similarity).
FT   VARIANT      48     48       G -> D (in a defective mutant).
SQ   SEQUENCE   586 AA;  65350 MW;  355B8C890E9F768D CRC64;
     MSQSSIIVVG GGLAGLMATI KAAESGMAVK LFSIVPVKRS HSVCAQGGIN GAVNTKGEGD
     SPWEHFDDTV YGGDFLANQP PLKAMCEAAP SIIHLLDRMA VMFNRTPEGL LDFRRFGGTQ
     HHRTAYAGAT TGQQLLYALD EQVRRYEVAG LVTKYEGWEF LGAVLDDDRT CRGIVAQNLT
     NMQIESFRSD AVIMATGGPG IIFGKSTNSM INTGSAASIV YQQGAYYANG EFIQIHPTAI
     PGDDKLRLMS ESARGEGGRV WTYKDGKPWY FLEEKYPAYG NLVPRDIATR EIFDVCVNQK
     LGINGENMVY LDLSHKDPKE LDIKLGGIIE IYEKFMGDDP RKLPMKIFPA VHYSMGGLWV
     DYDQMTNIPG LFAAGECDYS MHGGNRLERT SLLSAIYGGM VAGPNAVKYV NGLESSAEDM
     SSSLFDAHVK KEEEKWADIM IMDGTENAYV LHKELGEWMT ANVTVVRHND KLLKTDDKIQ
     ELMERFKKIN INDTTKWSNQ GAMFTRQFSN MLQLARVITL GAYNRNESRG AHYKPDYPER
     NDDEWLKTTM AKHVSPYEAP EFEYQDVDVS LITPRKRDYS KKKVAK
//