NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=Luria;
DOI=10.1016/0022-2836(87)90261-0; PubMed=3586029 [NCBI, ExPASy, EBI, Israel, Japan]
Schmitt M.P., Beck P.J., Kearney C.A., Spence J.L., Digiovanni D., Condreay J.P., Molineux I.J.;
"Sequence of a conditionally essential region of bacteriophage T3, including the primary origin of DNA replication.";
J. Mol. Biol. 193:479-495(1987).

Comments

FUNCTION: DNA ligase, which is expressed in the early stage of lytic development, has been implicated in T7 DNA synthesis and genetic recombination. It may also play a role in T7 DNA repair.
CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
SIMILARITY: Belongs to the ATP-dependent DNA ligase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X17255; CAA35125.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X05031; CAA28700.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S09539; S09539.

RefSeq

NP_523305.1; -.

3D structure databases

HSSP

P00969; 1A0I. [HSSP ENTRY / PDB]

SMR

P07717; 6-336.

ModBase

P07717.

Family and domain databases

InterPro

IPR012310; DNA_ligase_A_M.
IPR016059; DNA_ligase_CS.
IPR016306; DNA_ligase_phage.
IPR012340; NA-bd_OB-fold.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.50.140; OB_NA_bd_sub; 1.

Pfam

PF01068; DNA_ligase_A_M; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF001600; DNA_ligase_phage_T3; 1.

PROSITE

PS00697; DNA_LIGASE_A1; 1.
PS00333; DNA_LIGASE_A2; 1.
PS50160; DNA_LIGASE_A3; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

P07717.

Genome annotation databases

GeneID

927441; -.

Other

ProtoNet

P07717.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; DNA damage; DNA recombination; DNA repair; DNA replication; Ligase; Nucleotide-binding.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 346`	`346`	`DNA ligase.`	`PRO_0000059597`
`ACT_SITE`	`34 34`		`N6-AMP-lysine intermediate (By similarity).`

Sequence information

Length: 346 AA [This is the length of the unprocessed precursor]

Molecular weight: 39351 Da [This is the MW of the unprocessed precursor]

CRC64: 896D38E6AB80EE3B [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNIFNTNPFK AVSFVESAVK KALETSGYLI ADCKYDGVRG NIVVDNVAEA AWLSRVSKFI 

        70         80         90        100        110        120 
PALEHLNGFD KRWQQLLNDD RCIFPDGFML DGELMVKGVD FNTGSGLLRT KWVKRDNMGF 

       130        140        150        160        170        180 
HLTNVPTKLT PKGREVIDGK FEFHLDPKRL SVRLYAVMPI HIAESGEDYD VQNLLMPYHV 

       190        200        210        220        230        240 
EAMRSLLVEY FPEIEWLIAE TYEVYDMDSL TELYEEKRAE GHEGLIVKDP QGIYKRGKKS 

       250        260        270        280        290        300 
GWWKLKPECE ADGIIQGVNW GTEGLANEGK VIGFSVLLET GRLVDANNIS RALMDEFTSN 

       310        320        330        340 
VKAHGEDFYN GWACQVNYME ATPDGSLRHP SFEKFRGTED NPQEKM

P07717 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools