[1]	NUCLEOTIDE SEQUENCE [MRNA]. DOI=10.1021/bi00338a005; PubMed=2998455 [NCBI, ExPASy, EBI, Israel, Japan] Ashley P.L., MacDonald R.J.; "Kallikrein-related mRNAs of the rat submaxillary gland: nucleotide sequences of four distinct types including tonin."; Biochemistry 24:4512-4520(1985).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Prostate; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[3]	PROTEIN SEQUENCE OF 25-36 AND 112-122. TISSUE=Submandibular gland; PubMed=1900513 [NCBI, ExPASy, EBI, Israel, Japan] Yamaguchi T., Carretero O.A., Scicli A.G.; "A novel serine protease with vasoconstrictor activity coded by the kallikrein gene S3."; J. Biol. Chem. 266:5011-5017(1991).
[4]	PROTEIN SEQUENCE OF 25-47 AND 112-135, AND CHARACTERIZATION. PubMed=1315752 [NCBI, ExPASy, EBI, Israel, Japan] Moreau T., Brillard-Bourdet M., Bouhnik J., Gauthier F.; "Protein products of the rat kallikrein gene family. Substrate specificities of kallikrein rK2 (tonin) and kallikrein rK9."; J. Biol. Chem. 267:10045-10051(1992).
[5]	PROTEIN SEQUENCE OF 25-53 AND 112-130, AND CHARACTERIZATION. TISSUE=Submandibular gland; PubMed=1536657 [NCBI, ExPASy, EBI, Israel, Japan] Berg T., Schoeyen H., Wassdal I., Hull R., Gerskowitch V.P., Toft P.; "Characterization of a new kallikrein-like enzyme (KLP-S3) of the rat submandibular gland."; Biochem. J. 281:819-828(1992).

Comments

FUNCTION: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. This enzyme has a vasoconstrictor activity. KLK-9 has both a chymotrypsin-like and a trypsin-like properties.
CATALYTIC ACTIVITY: Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
SUBUNIT: Heterodimer of a light chain and heavy chain linked by a disulfide bond.
SIMILARITY: Belongs to the peptidase S1 family. Kallikrein subfamily [view classification].
SIMILARITY: Contains 1 peptidase S1 domain [view classification].

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M11566; AAA41467.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC061771; AAH61771.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

D23863; D23863.

NP_786935.1; -.

3D structure databases

HSSP

P00759; 1TON. [HSSP ENTRY / PDB]

SMR

P07647; 25-259.

ModBase

P07647.

Protein family/group databases

MEROPS

S01.407; -.

Organism-specific databases

RGD

727805; Klks3.

Gene expression databases

ArrayExpress

P07647; -.

GermOnline

ENSRNOG00000032857; Rattus norvegicus.

Family and domain databases

InterPro

IPR001254; Peptidase_S1_S6.
IPR001314; Peptidase_S1A.
Graphical view of domain structure.

Pfam

PF00089; Trypsin; 1.
Pfam graphical view of domain structure.

PRINTS

PR00722; CHYMOTRYPSIN.

SMART

SM00020; Tryp_SPc; 1.
SMART graphical view of domain structure.

PROSITE

PS50240; TRYPSIN_DOM; 1.
PS00134; TRYPSIN_HIS; 1.
PS00135; TRYPSIN_SER; 1.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

Ensembl

ENSRNOG00000032857; Rattus norvegicus. [Contig view]

GeneID

292868; -.

KEGG

rno:292868; -.

Phylogenomic databases

HOVERGEN

P07647; -.

Other

NextBio

634947; -.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; Glycoprotein; Hydrolase; Protease; Serine protease; Signal; Zymogen.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 18`	`18`	`Probable.`
`PROPEP`	`19 24`	`6`	`Activation peptide.`	`PRO_0000028009`
`CHAIN`	`25 259`	`235`	`Submandibular glandular kallikrein-9.`	`PRO_0000028010`
`CHAIN`	`25 111`	`87`	`Submandibular glandular kallikrein-9 light chain.`	`PRO_0000028011`
`CHAIN`	`112 259`	`148`	`Submandibular glandular kallikrein-9 heavy chain.`	`PRO_0000028012`
`DOMAIN`	`25 256`	`232`	`Peptidase S1.`
`ACT_SITE`	`63 63`		`Charge relay system.`
`ACT_SITE`	`118 118`		`Charge relay system.`
`ACT_SITE`	`211 211`		`Charge relay system.`
`CARBOHYD`	`106 106`		`N-linked (GlcNAc...) (Probable).`
`DISULFID`	`31 171`		`By similarity.`
`DISULFID`	`48 64`		`By similarity.`
`DISULFID`	`150 217`		`By similarity.`
`DISULFID`	`182 196`		`By similarity.`
`DISULFID`	`207 232`		`By similarity.`

Sequence information

Length: 259 AA [This is the length of the unprocessed precursor]

Molecular weight: 28368 Da [This is the MW of the unprocessed precursor]

CRC64: D167E8518BEC0791 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MWFLILFLAL SLGQIDAAPP GQSRVVGGYN CETNSQPWQV AVIGTTFCGG VLIDPSWVIT 

        70         80         90        100        110        120 
AAHCYSKNYR VLLGRNNLVK DEPFAQRRLV SQSFQHPDYI PVFMRNHTRQ RAYDHNNDLM 

       130        140        150        160        170        180 
LLHLSKPADI TGGVKVIDLP TEEPKVGSIC LASGWGMTNP SEMKLSHDLQ CVNIHLLSNE 

       190        200        210        220        230        240 
KCIETYKNIE TDVTLCAGEM DGGKDTCTGD SGGPLICDGV LQGLTSGGAT PCAKPKTPAI 

       250 
YAKLIKFTSW IKKVMKENP

P07647 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools