[1]	NUCLEOTIDE SEQUENCE [MRNA]. DOI=10.1093/nar/14.12.4823; PubMed=3636812 [NCBI, ExPASy, EBI, Israel, Japan] Fahnestock M., Brundage S., Shooter E.M.; "The sequence of a cDNA clone coding for a novel kallikrein from mouse submaxillary gland."; Nucleic Acids Res. 14:4823-4835(1986).
[2]	NUCLEOTIDE SEQUENCE OF 16-54 AND 70-122. PubMed=3036794 [NCBI, ExPASy, EBI, Israel, Japan] Evans B.A., Drinkwater C.C., Richards R.I.; "Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus."; J. Biol. Chem. 262:8027-8034(1987).

Comments

FUNCTION: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
CATALYTIC ACTIVITY: Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
SIMILARITY: Belongs to the peptidase S1 family. Kallikrein subfamily [view classification].
SIMILARITY: Contains 1 peptidase S1 domain [view classification].

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X03994; CAA27630.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M18587; AAA39347.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M18607; AAA39348.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

A24378; A24378.

NP_032483.1; -.

3D structure databases

HSSP

P36368; 1AO5. [HSSP ENTRY / PDB]

SMR

P07628; 25-261.

ModBase

P07628.

Protein family/group databases

MEROPS

S01.067; -.

Organism-specific databases

MGI

MGI:892018; Klk1b8.

Gene expression databases

ArrayExpress

P07628; -.

CleanEx

MM_KLK1B8; -.
MM_KLK8; -.

GermOnline

ENSMUSG00000063089; Mus musculus.

Family and domain databases

InterPro

IPR001254; Peptidase_S1_S6.
IPR001314; Peptidase_S1A.
Graphical view of domain structure.

Pfam

PF00089; Trypsin; 1.
Pfam graphical view of domain structure.

PRINTS

PR00722; CHYMOTRYPSIN.

SMART

SM00020; Tryp_SPc; 1.
SMART graphical view of domain structure.

PROSITE

PS50240; TRYPSIN_DOM; 1.
PS00134; TRYPSIN_HIS; 1.
PS00135; TRYPSIN_SER; 1.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

Ensembl

ENSMUSG00000063089; Mus musculus. [Contig view]

GeneID

16624; -.

KEGG

mmu:16624; -.

Phylogenomic databases

HOVERGEN

P07628; -.

Other

NextBio

290239; -.

SOURCE

Klk1b8; Mus musculus.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Glycoprotein; Hydrolase; Protease; Serine protease; Signal; Zymogen.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 18`	`18`	`Probable.`
`PROPEP`	`19 24`	`6`	`Activation peptide (Probable).`	`PRO_0000027977`
`CHAIN`	`25 261`	`237`	`Kallikrein 1-related peptidase b8.`	`PRO_0000027978`
`DOMAIN`	`25 258`	`234`	`Peptidase S1.`
`ACT_SITE`	`65 65`		`Charge relay system.`
`ACT_SITE`	`120 120`		`Charge relay system.`
`ACT_SITE`	`213 213`		`Charge relay system.`
`CARBOHYD`	`102 102`		`N-linked (GlcNAc...) (Probable).`
`DISULFID`	`31 173`		`By similarity.`
`DISULFID`	`50 66`		`By similarity.`
`DISULFID`	`152 219`		`By similarity.`
`DISULFID`	`184 198`		`By similarity.`
`DISULFID`	`209 234`		`By similarity.`

Sequence information

Length: 261 AA [This is the length of the unprocessed precursor]

Molecular weight: 28531 Da [This is the MW of the unprocessed precursor]

CRC64: D0174F7093137322 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MRFLILFLAL SLGGIDAAPP LQSRVVGGFN CEKNSQPWQV AVYDNKEHIC GGVLLERNWV 

        70         80         90        100        110        120 
LTAAHCYVDQ YEVWLGKNKL FQEEPSAQHR LVSKSFPHPG FNMSLLTLKE IPPGADFSND 

       130        140        150        160        170        180 
LMLLRLSKPA DITDAVKPIT LPTKESKLGS TCLASGWGSI TPTKWQKPDD LQCVFLKLLP 

       190        200        210        220        230        240 
IKNCIENHNV KVTDVMLCAG EMSGGKNICK GDSGGPLICD SVLQGITSTG PIPCGKPGVP 

       250        260 
AMYTNLIKFN SWIKDTMTKN S

P07628 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools