[1]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=Biotype B;
PubMed=7961420 [NCBI, ExPASy, EBI, Israel, Japan]
Kim S.W.,
Kim C.Y.,
Benisek W.F.,
Choi K.Y.;
"Cloning, nucleotide sequence, and overexpression of the gene coding for delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B.";
J. Bacteriol. 176:6672-6676(1994).
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[2]
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PROTEIN SEQUENCE.
STRAIN=Biotype B;
PubMed=3700400 [NCBI, ExPASy, EBI, Israel, Japan]
Linden K.G.,
Benisek W.F.;
"The amino acid sequence of a delta 5-3-oxosteroid isomerase from Pseudomonas putida biotype B.";
J. Biol. Chem. 261:6454-6460(1986).
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[3]
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X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH REACTION INTERMEDIATE ANALOG, AND MUTAGENESIS OF TYR-16 AND ASP-103.
DOI=10.1021/bi971546+; PubMed=9369474 [NCBI, ExPASy, EBI, Israel, Japan]
Kim S.-W.,
Cha S.-S.,
Cho H.-S.,
Kim J.-S.,
Ha N.-C.,
Cho M.-J.,
Joo S.,
Kim K.-K.,
Choi K.-Y.,
Oh B.-H.;
"High-resolution crystal structures of delta5-3-ketosteroid isomerase with and without a reaction intermediate analogue.";
Biochemistry 36:14030-14036(1997).
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[4]
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X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
DOI=10.1021/bi991040m; PubMed=10529226 [NCBI, ExPASy, EBI, Israel, Japan]
Kim D.-H.,
Nam G.H.,
Jang D.S.,
Choi G.,
Joo S.,
Kim J.-S.,
Oh B.-H.,
Choi K.-Y.;
"Roles of active site aromatic residues in catalysis by ketosteroid isomerase from Pseudomonas putida biotype B.";
Biochemistry 38:13810-13819(1999).
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[5]
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X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF MUTANT ASN-40/GLU-103 IN COMPLEX WITH REACTION INTERMEDIATE ANALOG, AND MUTAGENESIS OF TYR-16 AND ASP-103.
DOI=10.1021/bi991579k; PubMed=10653633 [NCBI, ExPASy, EBI, Israel, Japan]
Choi G.,
Ha N.-C.,
Kim S.W.,
Kim D.-H.,
Park S.,
Oh B.-H.,
Choi K.-Y.;
"Asp-99 donates a hydrogen bond not to Tyr-14 but to the steroid directly in the catalytic mechanism of delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B.";
Biochemistry 39:903-909(2000).
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[6]
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X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH SUBSTRATE.
DOI=10.1074/jbc.M007561200; PubMed=11007792 [NCBI, ExPASy, EBI, Israel, Japan]
Ha N.-C.,
Kim M.-S.,
Lee W.,
Choi K.-Y.,
Oh B.-H.;
"Detection of large pKa perturbations of an inhibitor and a catalytic group at an enzyme active site, a mechanistic basis for catalytic power of many enzymes.";
J. Biol. Chem. 275:41100-41106(2000).
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[7]
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X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF MUTANT SER-57, AND MUTAGENESIS OF TYR-16 AND TYR-32.
DOI=10.1021/bi015547k; PubMed=11695900 [NCBI, ExPASy, EBI, Israel, Japan]
Nam G.H.,
Jang D.S.,
Cha S.-S.,
Lee T.-H.,
Kim D.-H.,
Hong B.H.,
Yun Y.S.,
Oh B.-H.,
Choi K.-Y.;
"Maintenance of alpha-helical structures by phenyl rings in the active-site tyrosine triad contributes to catalysis and stability of ketosteroid isomerase from Pseudomonas putida biotype B.";
Biochemistry 40:13529-13537(2001).
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[8]
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X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF MUTANT ALA-92, AND MUTAGENESIS OF LEU-125 AND VAL-127.
DOI=10.1111/j.1742-4658.2005.04627.x; PubMed=15819891 [NCBI, ExPASy, EBI, Israel, Japan]
Yun Y.S.,
Nam G.H.,
Kim Y.-G.,
Oh B.-H.,
Choi K.-Y.;
"Small exterior hydrophobic cluster contributes to conformational stability and steroid binding in ketosteroid isomerase from Pseudomonas putida biotype B.";
FEBS J. 272:1999-2011(2005).
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