[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=3517852 [NCBI, ExPASy, EBI, Israel, Japan] Broyles S.S., Moss B.; "Homology between RNA polymerases of poxviruses, prokaryotes, and eukaryotes: nucleotide sequence and transcriptional analysis of vaccinia virus genes encoding 147-kDa and 22-kDa subunits."; Proc. Natl. Acad. Sci. U.S.A. 83:3141-3145(1986).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-111. DOI=10.1093/nar/13.3.985; PubMed=2987815 [NCBI, ExPASy, EBI, Israel, Japan] Plucienniczak A., Schroeder E., Zettlmeissl G., Streeck R.E.; "Nucleotide sequence of a cluster of early and late genes in a conserved segment of the vaccinia virus genome."; Nucleic Acids Res. 13:985-998(1985).

Comments

FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
SUBUNIT: This enzyme consists of at least eight subunits.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M13209; AAB59835.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X01978; CAA26020.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

B25734; RNVZ47.

3D structure databases

ModBase

P07392.

Family and domain databases

InterPro

IPR000722; RNA_pol_A.
IPR006592; RNA_pol_N.
IPR007066; RNA_pol_Rpb1_3.
IPR007083; RNA_pol_Rpb1_4.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.40.30; RNA_pol_A; 1.

Pfam

PF00623; RNA_pol_Rpb1_2; 1.
PF04983; RNA_pol_Rpb1_3; 1.
PF05000; RNA_pol_Rpb1_4; 1.
Pfam graphical view of domain structure.

SMART

SM00663; RPOLA_N; 1.
SMART graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription; Transferase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1287`	`1287`	`DNA-directed RNA polymerase 147 kDa polypeptide.`	`PRO_0000073920`
`CONFLICT`	`62 62`		`H -> Q (in Ref. 2; CAA26020).`
`CONFLICT`	`83 83`		`I -> T (in Ref. 2; CAA26020).`

Sequence information

Length: 1287 AA [This is the length of the unprocessed precursor]

Molecular weight: 146949 Da [This is the MW of the unprocessed precursor]

CRC64: 570BB7DA1D4EEB76 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAVISKVTYS LYDQKEINAT DIIISHVKND DDIGTVKDGR LGAMDGALCK TCGKTELECF 

        70         80         90        100        110        120 
GHWGKVSIYK THIVKPEFIS EIIRLLNHIC IHCGLLRSRE PYSDDINLKE LSGHALRRLK 

       130        140        150        160        170        180 
DKILSKKKSC WNSECMQPYQ KITFSKKKVC FVNKLDDINV PNSLIYQKLI SIHEKFWPLL 

       190        200        210        220        230        240 
EIHQYPANLF YTDYFPIPPL IIRPAISFWI DSIPKETNEL TYLLGMIVKN CNLNADEQVI 

       250        260        270        280        290        300 
QKAVIEYDDI KIISNNTSSI NLSYITSGKN NMIRSYIVAR RKDQTARSVI GPSTSITVNE 

       310        320        330        340        350        360 
VGMPAYIRNT LTEKIFVNAF TVDKVKQLLA SNQVKFYFNK RLNQLTRIRQ GKFIKNKIHL 

       370        380        390        400        410        420 
LPGDWVEVAV QEYTSIIFGR QPSLHRYNVI ASSIRATEGD TIKISPGIAN SQNADFDGDE 

       430        440        450        460        470        480 
EWMILEQNPK AVIEQSILMY PTTLLKHDIH GAPVYGSIQD EIVAAYSLFR IQDLCLDEVL 

       490        500        510        520        530        540 
NILGKYGREF DPKGKCKFSG KDIYTYLIGE KINYPGLLKD GEIIANDVDS NFVVAMRHLS 

       550        560        570        580        590        600 
LAGLLSDHKS NVEGINFIIK SSYVFKRYLS IYGFGVTFKD LRPNSTFTNK LEAINVEKIE 

       610        620        630        640        650        660 
LIKEAYAKYL NDVRDGKIVP LSKALEADYV ESMLSNLTNL NIREIEEHMR QTLIDDPDNN 

       670        680        690        700        710        720 
LLKMAKAGYK VNPTELMYIL GTYGQQRIDG EPAETRVLGR VLPYYLPDSK DPEGRGYILN 

       730        740        750        760        770        780 
SLTKGLTGSQ YYFSMLVARS QSTDIVCETS RTGTLARKII KKMEDMVVDG YGQVVIGNTL 

       790        800        810        820        830        840 
IKYAANYTKI LGSVCKPVDL IYPDESMTWY LEISALWNKI KQGFVYSQKQ KLAKKTLAPF 

       850        860        870        880        890        900 
NFLVFVKPTT EDNAIKVKDL YDMIHNVIDD VREKYFFTVS NIDFMEYIFL THLNPSRIRI 

       910        920        930        940        950        960 
TKETAITIFE KFYEKLNYTL GGGTPIGIIS AQVLSEKFTQ QALSSFHTTE KSGAVKQKLG 

       970        980        990       1000       1010       1020 
FNEFNNLTNL SKNKTEIITL VSDDISKLQS VKINFEFVCL GELNPDHSLF EKKQDRYVVD 

      1030       1040       1050       1060       1070       1080 
IIVNKLYIKR AEITELVVEY MIERFISFSV IVKEWGMETF IEDEDNIRFT VYLNFVEPEE 

      1090       1100       1110       1120       1130       1140 
LNLSKFMMVL PGAANKGKIS KFKIPISDYT GYDCFNQTKK LNKMTVELMN LKELGSFDLE 

      1150       1160       1170       1180       1190       1200 
NVNVYPGVWN TYDIFGIEAA REYLCEAMLN TYGEGFDYLY QPCDLLASLL CASYEPESVN 

      1210       1220       1230       1240       1250       1260 
KFKFGAASTL KRATFGDNKA LLNAALHKKS EPINDNSSCH FFSKVPNIGT GYYKYFIDLG 

      1270       1280 
LLMRMERKLS DKISSQKIKE MEETEDF

P07392 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools