[1]
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NUCLEOTIDE SEQUENCE [MRNA].
PubMed=2865729 [NCBI, ExPASy, EBI, Israel, Japan]
Sakimura K.,
Kushiya E.,
Obinata M.,
Odani S.,
Takahashi Y.;
"Molecular cloning and the nucleotide sequence of cDNA for neuron-specific enolase messenger RNA of rat brain.";
Proc. Natl. Acad. Sci. U.S.A. 82:7453-7457(1985).
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[2]
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NUCLEOTIDE SEQUENCE.
STRAIN=Sprague-Dawley;
TISSUE=Brain;
PubMed=3746946 [NCBI, ExPASy, EBI, Israel, Japan]
Forss-Petter S.,
Danielson P.,
Sutcliffe J.G.;
"Neuron-specific enolase: complete structure of rat mRNA, multiple transcriptional start sites, and evidence suggesting post-transcriptional control.";
J. Neurosci. Res. 16:141-156(1986).
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[3]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Pituitary;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
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[4]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
DOI=10.1016/0378-1119(87)90218-6; PubMed=2450052 [NCBI, ExPASy, EBI, Israel, Japan]
Sakimura K.,
Kushiya E.,
Takahashi Y.,
Suzuki Y.;
"The structure and expression of neuron-specific enolase gene.";
Gene 60:103-113(1987).
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[5]
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PROTEIN SEQUENCE OF 10-28; 32-50; 65-89; 106-120; 163-179; 184-193; 240-262; 270-285; 307-326; 336-394 AND 407-422, AND MASS SPECTROMETRY.
STRAIN=Sprague-Dawley;
TISSUE=Brain, Hippocampus, and Spinal cord;
Lubec G.,
Afjehi-Sadat L.,
Chen W.-Q.,
Kang S.U.;
Submitted (JUL-2007) to UniProtKB.
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[6]
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POSSIBLE FUNCTION AS A NEUROTROPHIC FACTOR.
DOI=10.1016/0168-0102(94)00849-B; PubMed=7753500 [NCBI, ExPASy, EBI, Israel, Japan]
Hattori T.,
Takei N.,
Mizuno Y.,
Kato K.,
Kohsaka S.;
"Neurotrophic and neuroprotective effects of neuron-specific enolase on cultured neurons from embryonic rat brain.";
Neurosci. Res. 21:191-198(1995).
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[7]
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SUBCELLULAR LOCATION OF ALPHA/GAMMA HETERODIMER.
DOI=10.1016/j.neures.2003.12.006; PubMed=15041191 [NCBI, ExPASy, EBI, Israel, Japan]
Ueta H.,
Nagasawa H.,
Oyabu-Manabe Y.,
Toida K.,
Ishimura K.,
Hori H.;
"Localization of enolase in synaptic plasma membrane as an alphagamma heterodimer in rat brain.";
Neurosci. Res. 48:379-386(2004).
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- FUNCTION: Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
- CATALYTIC ACTIVITY: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O.
- COFACTOR: Magnesium. Required for catalysis and for stabilizing the dimer.
- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
- SUBUNIT: Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.
- SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell membrane (By similarity). Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form (By similarity).
- TISSUE SPECIFICITY: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
- DEVELOPMENTAL STAGE: During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. ENO2 levels in brain increase 10-30 days after birth. Levels continue to accumulate over the following few months (protein only).
- SIMILARITY: Belongs to the enolase family.
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