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UniProtKB/Swiss-Prot entry P07288


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name KLK3_HUMAN
Primary accession number P07288
Secondary accession numbers Q16272 Q86TG8
Integrated into Swiss-Prot on April 1, 1988
Sequence was last modified on July 1, 1989 (Sequence version 2)
Annotations were last modified on    November 4, 2008 (Entry version 108)
Name and origin of the protein
Protein name Prostate-specific antigen [Precursor]
Synonyms PSA
EC 3.4.21.77
Kallikrein-3
Semenogelase
Gamma-seminoprotein
Seminin
P-30 antigen
Gene name
Name: KLK3
Synonyms: APS
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Prostate;
DOI=10.1016/0014-5793(87)80078-9; PubMed=2436946 [NCBI, ExPASy, EBI, Israel, Japan]
Lundwall A., Lilja H.;
"Molecular cloning of human prostate specific antigen cDNA.";
FEBS Lett. 214:317-322(1987).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
TISSUE=Prostate;
DOI=10.1093/nar/17.5.2137; PubMed=2467258 [NCBI, ExPASy, EBI, Israel, Japan]
Digby M.R., Zhang X.Y., Richard R.I.;
"Human prostate specific antigen (PSA) gene: structure and linkage to the kallikrein-like gene, hGK-1.";
Nucleic Acids Res. 17:2137-2137(1989).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1093/nar/17.10.3981; PubMed=2471958 [NCBI, ExPASy, EBI, Israel, Japan]
Klobeck H.-G., Combriato G., Schulz P., Arbusow V., Fittler F.;
"Genomic sequence of human prostate specific antigen (PSA).";
Nucleic Acids Res. 17:3981-3981(1989).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
TISSUE=Leukocyte;
DOI=10.1016/0006-291X(89)91362-4; PubMed=2472789 [NCBI, ExPASy, EBI, Israel, Japan]
Lundwall A.;
"Characterization of the gene for prostate-specific antigen, a human glandular kallikrein.";
Biochem. Biophys. Res. Commun. 161:1151-1159(1989).
[5]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Prostate;
DOI=10.1016/0006-291X(89)92520-5; PubMed=2470373 [NCBI, ExPASy, EBI, Israel, Japan]
Henttu P., Vihko P.;
"cDNA coding for the entire human prostate specific antigen shows high homologies to the human tissue kallikrein genes.";
Biochem. Biophys. Res. Commun. 160:903-910(1989).
[6]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
TISSUE=Prostate;
DOI=10.1016/0006-291X(89)92409-1; PubMed=2466464 [NCBI, ExPASy, EBI, Israel, Japan]
Riegman P.H.J., Vlietstra R.J., van der Korput J.A.G.M., Romijn J.C., Trapman J.;
"Characterization of the prostate-specific antigen gene: a novel human kallikrein-like gene.";
Biochem. Biophys. Res. Commun. 159:95-102(1989).
[7]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Prostate;
Monne M.M., Moreno J.M., Mele C.M., Mulholland G.M., Gomella L.G.;
"A comparative analysis of prostate specific antigen gene sequence in benign and malignant prostate tissue.";
Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases.
[8]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/S0378-1119(00)00382-6; PubMed=11054574 [NCBI, ExPASy, EBI, Israel, Japan]
Gan L., Lee I., Smith R., Argonza-Barrett R., Lei H., McCuaig J., Moss P., Paeper B., Wang K.;
"Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region.";
Gene 257:119-130(2000).
[9]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.;
"Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
[10]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=PNS, and Prostate;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[11]
NUCLEOTIDE SEQUENCE [MRNA] OF 1-176.
PubMed=7527295 [NCBI, ExPASy, EBI, Israel, Japan]
Monne M., Croce C.M., Yu H., Diamandis E.P.;
"Molecular characterization of prostate-specific antigen messenger RNA expressed in breast tumors.";
Cancer Res. 54:6344-6347(1994).
[12]
NUCLEOTIDE SEQUENCE [MRNA] OF 17-261.
DOI=10.1093/nar/16.13.6226; PubMed=2456523 [NCBI, ExPASy, EBI, Israel, Japan]
Schulz P., Stucka R., Feldmann H., Combriato G., Klobeck H.-G., Fittler F.;
"Sequence of a cDNA clone encompassing the complete mature human prostate specific antigen (PSA) and an unspliced leader sequence.";
Nucleic Acids Res. 16:6226-6226(1988).
[13]
PROTEIN SEQUENCE OF 25-261.
PubMed=2422647 [NCBI, ExPASy, EBI, Israel, Japan]
Watt K.W.K., Lee P.J., M'Timkulu T., Chan W.P., Loor R.;
"Human prostate-specific antigen: structural and functional similarity with serine proteases.";
Proc. Natl. Acad. Sci. U.S.A. 83:3166-3170(1986).
[14]
PROTEIN SEQUENCE OF 25-261.
PubMed=3691515 [NCBI, ExPASy, EBI, Israel, Japan]
Schaller J., Akiyama K., Tsuda R., Hara M., Marti T., Rickli E.E.;
"Isolation, characterization and amino-acid sequence of gamma-seminoprotein, a glycoprotein from human seminal plasma.";
Eur. J. Biochem. 170:111-120(1987).
[15]
PROTEIN SEQUENCE OF 18-32.
DOI=10.1110/ps.04682504; PubMed=15340161 [NCBI, ExPASy, EBI, Israel, Japan]
Zhang Z., Henzel W.J.;
"Signal peptide prediction based on analysis of experimentally verified cleavage sites.";
Protein Sci. 13:2819-2824(2004).
[16]
3D-STRUCTURE MODELING.
PubMed=7535613 [NCBI, ExPASy, EBI, Israel, Japan]
Villoutreix B.O., Getzoff E.D., Griffin J.H.;
"A structural model for the prostate disease marker, human prostate-specific antigen.";
Protein Sci. 3:2033-2044(1994).
[17]
3D-STRUCTURE MODELING.
DOI=10.1016/S1074-5521(98)90004-7; PubMed=9751643 [NCBI, ExPASy, EBI, Israel, Japan]
Coombs G.S., Bergstrom R.C., Pellequer J.L., Baker S.I., Navre M., Smith M.M., Tainer J.A., Madison E.L., Corey D.R.;
"Substrate specificity of prostate-specific antigen (PSA).";
Chem. Biol. 5:475-488(1998).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X05332; CAA28947.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X13940; CAA32123.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X13941; CAA32124.1; ALT_SEQ; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X13942; CAB46487.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X13943; CAA32126.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X13944; CAA32127.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X14810; CAA32915.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M27274; AAA60192.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M26663; AAA58802.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M24543; AAA60193.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U17040; AAA56764.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF243527; AAG33355.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT019862; AAV38665.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC005307; AAH05307.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC050595; AAH50595.2; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC056665; AAH56665.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S75755; AAD14185.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X07730; -; NOT_ANNOTATED_CDS; mRNA.[EMBL / GenBank / DDBJ]
PIR A32297; A32297.
RefSeq NP_001025218.1; -.
NP_001025219.1; -.
NP_001025220.1; -.
NP_001639.1; -.
UniGene Hs.171995
3D structure databases
PDB
1PFA; Model; -; A=10-261.[ExPASy / RCSB / EBI]
2PSA; Model; -; A=25-261.[ExPASy / RCSB / EBI]
2ZCH; X-ray; 2.83 A; P=25-261.[ExPASy / RCSB / EBI]
2ZCK; X-ray; 3.10 A; P=25-261.[ExPASy / RCSB / EBI]
2ZCL; X-ray; 3.25 A; P=25-261.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1PFA; -.
2PSA; -.
2ZCH; -.
2ZCK; -.
2ZCL; -.
ModBase P07288.
Protein family/group databases
MEROPS S01.162; -.
Organism-specific databases
H-InvDB HIX0015369; -.
HGNC HGNC:6364; KLK3.
GenAtlas KLK3.
HPA CAB000070; -.
HPA000764; -.
HPA000829; -.
MIM 176820; gene. [NCBI / EBI]
PharmGKB PA30153; -.
GeneCards P07288.
Gene expression databases
ArrayExpress P07288; -.
CleanEx HS_KLK3; -.
GermOnline ENSG00000142515; Homo sapiens.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from direct assay from HPA).
GO:0005576; Cellular component: extracellular region (non-traceable author statement from UniProtKB).
GO:0005634; Cellular component: nucleus (inferred from direct assay from HPA).
GO:0008236; Molecular function: serine-type peptidase activity (traceable author statement from ProtInc).
GO:0016525; Biological process: negative regulation of angiogenesis (non-traceable author statement from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR001254; Peptidase_S1_S6.
IPR001314; Peptidase_S1A.
Graphical view of domain structure.
Pfam PF00089; Trypsin; 1.
Pfam graphical view of domain structure.
PRINTS PR00722; CHYMOTRYPSIN.
SMART SM00020; Tryp_SPc; 1.
SMART graphical view of domain structure.
PROSITE PS50240; TRYPSIN_DOM; 1.
PS00134; TRYPSIN_HIS; 1.
PS00135; TRYPSIN_SER; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P07288.
ProtoNet P07288.
Genome annotation databases
Ensembl ENSG00000142515; Homo sapiens. [Contig view]
GeneID 354; -.
KEGG hsa:354; -.
Phylogenomic databases
HOGENOM P07288; -.
HOVERGEN P07288; -.
Other
NextBio 1459; -.
SOURCE KLK3; Homo sapiens.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Direct protein sequencing; Glycoprotein; Hydrolase; Polymorphism; Protease; Secreted; Serine protease; Signal; Zymogen.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    17  17      
PROPEP   18    24  7     Activation peptide. PRO_0000027931
CHAIN   25   261  237     Prostate-specific antigen. PRO_0000027932
DOMAIN   25   258  234     Peptidase S1. 
ACT_SITE   65    65        Charge relay system. 
ACT_SITE   120   120        Charge relay system. 
ACT_SITE   213   213        Charge relay system. 
CARBOHYD   69    69        N-linked (GlcNAc...). 
DISULFID   31   173         
DISULFID   50    66         
DISULFID   152   219         
DISULFID   184   198         
DISULFID   209   234         
VARIANT   32    32  1     E -> K (in dbSNP:rs2271092 [NCBI]). VAR_021941 
VARIANT   132   132  1     L -> I (in dbSNP:rs2003783 [NCBI]). VAR_021942 
CONFLICT   64    64        A -> T (in Ref. 12). 
CONFLICT   69    69        N -> KC (in Ref. 6; AAA60193). 
CONFLICT   94    94        H -> T (in Ref. 13; AA sequence). 
CONFLICT   136   136        V -> M (in Ref. 12). 
CONFLICT   165   168        FLTP -> HLLYDQM (in Ref. 13; AA sequence). 
CONFLICT   175   175        D -> Q (in Ref. 13; AA sequence). 
STRAND   39    56  18      
STRAND   59    62  4      
HELIX   64    66  3      
STRAND   72    76  5      
STRAND   78    82  5      
STRAND   88    97  10      
HELIX   103   106  4      
STRAND   107   110  4      
STRAND   122   128  7      
STRAND   151   157  7      
STRAND   160   164  5      
STRAND   172   179  8      
HELIX   181   187  7      
STRAND   189   191  3      
STRAND   196   200  5      
STRAND   216   229  14      
STRAND   241   245  5      
HELIX   246   249  4      
HELIX   250   258  9      
Sequence information
Length: 261 AA [This is the length of the unprocessed precursor] Molecular weight: 28741 Da [This is the MW of the unprocessed precursor] CRC64: AE9E732AF872141A [This is a checksum on the sequence]
        10         20         30         40         50         60 
MWVPVVFLTL SVTWIGAAPL ILSRIVGGWE CEKHSQPWQV LVASRGRAVC GGVLVHPQWV 

        70         80         90        100        110        120 
LTAAHCIRNK SVILLGRHSL FHPEDTGQVF QVSHSFPHPL YDMSLLKNRF LRPGDDSSHD 

       130        140        150        160        170        180 
LMLLRLSEPA ELTDAVKVMD LPTQEPALGT TCYASGWGSI EPEEFLTPKK LQCVDLHVIS 

       190        200        210        220        230        240 
NDVCAQVHPQ KVTKFMLCAG RWTGGKSTCS GDSGGPLVCN GVLQGITSWG SEPCALPERP 

       250        260 
SLYTKVVHYR KWIKDTIVAN P 

P07288 in FASTA format

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View entry in raw text format (no links)
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BLAST logo BLAST submission on ExPASy/SIB
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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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