[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. DOI=10.1016/0378-1119(85)90208-2; PubMed=3934045 [NCBI, ExPASy, EBI, Israel, Japan] Uchiyama H., Weisblum B.; "N-methyl transferase of Streptomyces erythraeus that confers resistance to the macrolide-lincosamide-streptogramin B antibiotics: amino acid sequence and its homology to cognate R-factor enzymes from pathogenic bacilli and cocci."; Gene 38:103-110(1985).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. DOI=10.1016/0014-5793(90)80186-M; PubMed=2335200 [NCBI, ExPASy, EBI, Israel, Japan] Dhillon N., Leadlay P.F.; "A repeated decapeptide motif in the C-terminal domain of the ribosomal RNA methyltransferase from the erythromycin producer Saccharopolyspora erythraea."; FEBS Lett. 262:189-193(1990).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. DOI=10.1038/nbt1297; PubMed=17369815 [NCBI, ExPASy, EBI, Israel, Japan] Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F.; "Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338."; Nat. Biotechnol. 25:447-453(2007).
[4]	NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-133. DOI=10.1016/0378-1119(85)90220-3; PubMed=2998943 [NCBI, ExPASy, EBI, Israel, Japan] Bibb M.J., Janssen G.R., Ward J.M.; "Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus."; Gene 38:215-226(1985).

Comments

FUNCTION: This protein produces a dimethylation of the adenine residue at position 2058 in 23S rRNA, resulting in reduced affinity between ribosomes and macrolide-lincosamide-streptogramin B antibiotics.
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N⁶-methyladenine.
SIMILARITY: Belongs to the rRNA adenine N(6)-methyltransferase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M11200; AAA26492.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AM420293; CAM00074.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X51891; CAB60001.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A91534; XYSMRE.

RefSeq

YP_001103000.1; -.

3D structure databases

ModBase

P07287.

Ontologies

GO:0008988;	Molecular function: rRNA (adenine-N6-)-methyltransferase activity (inferred from electronic annotation from EC).
GO:0046677;	Biological process: response to antibiotic (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001737; RRNA_meth_trans.
Graphical view of domain structure.

PROSITE

PS01131; RRNA_A_DIMETH; 1.

Genome annotation databases

GeneID

4940594; -.

GenomeReviews

AM420293_GR; SACE_0733.

KEGG

sen:SACE_0733; -.

CMR

P07287; SACE_0733.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Antibiotic resistance; Complete proteome; Methyltransferase; S-adenosyl-L-methionine; Transferase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 381`	`381`	`rRNA adenine N-6-methyltransferase.`	`PRO_0000101677`
`COMPBIAS`	`291 379`	`89`	`Arg/Gly-rich.`
`CONFLICT`	`71 71`		`G -> V (in Ref. 1; AAA26492).`
`CONFLICT`	`191 201`		`Missing (in Ref. 1; AAA26492).`
`CONFLICT`	`257 257`		`L -> F (in Ref. 1; AAA26492).`

Sequence information

Length: 381 AA [This is the length of the unprocessed precursor]

Molecular weight: 43001 Da [This is the MW of the unprocessed precursor]

CRC64: 17A7C78CAD865FA9 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSSSDEQPRP RRRNQDRQHP NQNRPVLGRT ERDRNRRQFG QNFLRDRKTI ARIAETAELR 

        70         80         90        100        110        120 
PDLPVLEAGP GEGLLTRELA DRARQVTSYE IDPRLAKSLR EKLSGHPNIE VVNADFLTAE 

       130        140        150        160        170        180 
PPPEPFAFVG AIPYGITSAI VDWCLEAPTI ETATMVTQLE FARKRTGDYG RWSRLTVMTW 

       190        200        210        220        230        240 
PLFEWEFVEK VDRRLFKPVP KVDSAIMRLR RRAEPLLEGA ALERYESMVE LCFTGVGGNI 

       250        260        270        280        290        300 
QASLLRKYPR RRVEAALDHA GVGGGAVVAY VRPEQWLRLF ERLDQKNEPR GGQPQRGRRT 

       310        320        330        340        350        360 
GGRDHGDRRT GGQDRGDRRT GGRDHRDRQA SGHGDRRSSG RNRDDGRTGE REQGDQGGRR 

       370        380 
GPSGGGRTGG RPGRRGGPGQ R

P07287 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools