ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry P07275


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name PUT2_YEAST
Primary accession number P07275
Secondary accession numbers None
Integrated into Swiss-Prot on April 1, 1988
Sequence was last modified on February 1, 1995 (Sequence version 2)
Annotations were last modified on    November 4, 2008 (Entry version 92)
Name and origin of the protein
Protein name Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial [Precursor]
Synonyms P5C dehydrogenase
EC 1.5.1.12
Gene name
Name: PUT2
OrderedLocusNames: YHR037W
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=6098824 [NCBI, ExPASy, EBI, Israel, Japan]
Krzywicki K.A., Brandriss M.C.;
"Primary structure of the nuclear PUT2 gene involved in the mitochondrial pathway for proline utilization in Saccharomyces cerevisiae.";
Mol. Cell. Biol. 4:2837-2842(1984).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204511 / S288c / AB972;
PubMed=8091229 [NCBI, ExPASy, EBI, Israel, Japan]
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.;
"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.";
Science 265:2077-2082(1994).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-3.
PubMed=3062363 [NCBI, ExPASy, EBI, Israel, Japan]
Siddiqui A.H., Brandriss M.C.;
"A regulatory region responsible for proline-specific induction of the yeast PUT2 gene is adjacent to its TATA box.";
Mol. Cell. Biol. 8:4634-4641(1988).
[4]
SUBCELLULAR LOCATION.
DOI=10.1021/bi010277r; PubMed=11502169 [NCBI, ExPASy, EBI, Israel, Japan]
Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.;
"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex.";
Biochemistry 40:9758-9769(2001).
[5]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M10029; AAA34924.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M22785; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
U00062; AAB68907.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S46738; RDBYC.
RefSeq NP_011902.1; -.
3D structure databases
ModBase P07275.
Protein-protein interaction databases
IntAct P07275; -.
Enzyme and pathway databases
BioCyc MetaCyc:MON-646; -.
Organism-specific databases
CYGD YHR037w; -.
SGD S000001079; PUT2.
Yeast-GFP YHR037W.
Gene expression databases
GermOnline YHR037W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005739; Cellular component: mitochondrion (inferred from direct assay from SGD).
GO:0003842; Molecular function: 1-pyrroline-5-carboxylate dehydrogenase activity (inferred from mutant phenotype from SGD).
GO:0042802; Molecular function: identical protein binding (inferred from physical interaction from IntAct).
GO:0006537; Biological process: glutamate biosynthetic process (traceable author statement from SGD).
GO:0010133; Biological process: proline catabolic process to glutamate (inferred from mutant phenotype from SGD).
QuickGo view.
Family and domain databases
InterPro IPR016160; Ald_DHase_CS.
IPR016162; Ald_DHase_N.
IPR015590; Aldehyde_DHase.
IPR005931; d-1-pyrroline-5-COlate_DHase-1.
Graphical view of domain structure.
Gene3D G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.
PANTHER PTHR11699; Aldehyde_dehyd; 1.
Pfam PF00171; Aldedh; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR01236; D1pyr5carbox1; 1.
PROSITE PS00070; ALDEHYDE_DEHYDR_CYS; 1.
PS00687; ALDEHYDE_DEHYDR_GLU; 1.
BLOCKS P07275.
ProtoNet P07275.
Proteomic databases
PeptideAtlas P07275; -.
Genome annotation databases
Ensembl YHR037W; Saccharomyces cerevisiae. [Contig view]
GeneID 856432; -.
GenomeReviews U00093_GR; YHR037W.
KEGG sce:YHR037W; -.
NMPDR fig|4932.3.peg.3051; -.
Phylogenomic databases
HOGENOM P07275; -.
Other
LinkHub P07275; -.
NextBio 982024; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Membrane; Mitochondrion; Mitochondrion inner membrane; NAD; Oxidoreductase; Proline metabolism; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1     ?        Mitochondrion (Potential). 
CHAIN   ?   575        Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial. PRO_0000007177
NP_BIND   297   302  6     NAD (By similarity). 
ACT_SITE   317   317        Proton acceptor (By similarity). 
ACT_SITE   351   351        Nucleophile (By similarity). 
SITE   212   212  1     Transition state stabilizer (By similarity). 
CONFLICT   187   188        ES -> SR (in Ref. 1; AAA34924). 
CONFLICT   264   264        P -> L (in Ref. 1; AAA34924). 
CONFLICT   541   541        D -> G (in Ref. 1; AAA34924). 
CONFLICT   561   561        N -> S (in Ref. 1; AAA34924). 
Sequence information
Length: 575 AA [This is the length of the unprocessed precursor] Molecular weight: 64435 Da [This is the MW of the unprocessed precursor] CRC64: 5A610D67985291B0 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MLSARCLKSI YFKRSFSQLG HIKPPKHIRN EPVKPFRNID LKDWDLLRAS LMKFKSSSLE 

        70         80         90        100        110        120 
VPLVINGERI YDNNERALFP QTNPANHQQV LANVTQATEK DVMNAVKAAK DAKKDWYNLP 

       130        140        150        160        170        180 
FYDRSAIFLK AADLISTKYR YDMLAATMLG QGKNVYQAEI DCITELSDFF RYYVKYASDL 

       190        200        210        220        230        240 
YAQQPVESAD GTWNKAEYRP LEGFVYAVSP FNFTAIAANL IGAPALMGNT VVWKPSQTAA 

       250        260        270        280        290        300 
LSNYLLMTVL EEAGLPKGVI NFIPGDPVQV TDQVLADKDF GALHFTGSTN VFKSLYGKIQ 

       310        320        330        340        350        360 
SGVVEGKYRD YPRIIGETGG KNFHLVHPSA NISHAVLSTI RGTFEFQGQK CSAASRLYLP 

       370        380        390        400        410        420 
ESKSEEFLSD MFGILQSQNV VPMNTSASPI SGGNLRGFMG PVIHEQSFDK LVKVIEDAKK 

       430        440        450        460        470        480 
DPELEILYGG QYDKSQGWFV GPTVIKAKRP DHPYMSTEFF GPILTVYEYP DTEFNEICDI 

       490        500        510        520        530        540 
IDNTSQYALT GAIFAKDRKA IEYADEKLKF SAGNFYINDK CTGAVVSQQW FGGARMSGTD 

       550        560        570 
DKAGGPNILS RFVSIRNTKE NFYELTDFKY PSNYE 

P07275 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by ch flag SIB Switzerland Mirror sites: Australia  Brazil  Canada  China  Korea
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!