ID   SYH_YEAST               Reviewed;         546 AA.
AC   P07263; Q12095;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   16-DEC-2008, entry version 99.
DE   RecName: Full=Histidyl-tRNA synthetase, mitochondrial;
DE            EC=6.1.1.21;
DE   AltName: Full=Histidine--tRNA ligase;
DE            Short=HisRS;
DE   Flags: Precursor;
GN   Name=HTS1; OrderedLocusNames=YPR033C; ORFNames=YP9367.13C;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=86245069; PubMed=3521891; DOI=10.1016/0092-8674(86)90740-3;
RA   Natsoulis G., Hilger F., Fink G.R.;
RT   "The HTS1 gene encodes both the cytoplasmic and mitochondrial
RT   histidine tRNA synthetases of S. cerevisiae.";
RL   Cell 46:235-243(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204511 / S288c / AB972;
RX   MEDLINE=97313271; PubMed=9169875;
RA   Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA   Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V.,
RA   Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M.,
RA   Chung E., Churcher C.M., Coster F., Davis K., Davis R.W.,
RA   Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A.,
RA   Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A.,
RA   Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W.,
RA   Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K.,
RA   Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J.,
RA   Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D.,
RA   Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA   Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA   Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA   Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA   Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V.,
RA   Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W.,
RA   Zollner A., Vo D.H., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL   Nature 387:103-105(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A.,
RA   Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F.,
RA   Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G.,
RA   Kolodner R.D., LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-
RT   encoding clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   MUTAGENESIS.
RX   MEDLINE=93093458; PubMed=1459448;
RA   Chiu M.I., Mason T.L., Fink G.R.;
RT   "HTS1 encodes both the cytoplasmic and mitochondrial histidyl-tRNA
RT   synthetase of Saccharomyces cerevisiae: mutations alter the
RT   specificity of compartmentation.";
RL   Genetics 132:987-1001(1992).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- CATALYTIC ACTIVITY: ATP + L-histidine + tRNA(His) = AMP +
CC       diphosphate + L-histidyl-tRNA(His).
CC   -!- INTERACTION:
CC       P38080:AKL1; NbExp=1; IntAct=EBI-18711, EBI-9528;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion. Cytoplasm.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=Mitochondrial;
CC         IsoId=P07263-1; Sequence=Displayed;
CC       Name=Cytoplasmic;
CC         IsoId=P07263-2; Sequence=VSP_018907;
CC   -!- MISCELLANEOUS: Present with 26800 molecules/cell in log phase SD
CC       medium.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase
CC       family.
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DR   EMBL; M14048; AAA34695.1; -; Genomic_DNA.
DR   EMBL; M14048; AAA34696.1; -; Genomic_DNA.
DR   EMBL; Z71255; CAA94983.1; -; Genomic_DNA.
DR   EMBL; Z49274; CAA89287.1; -; Genomic_DNA.
DR   EMBL; AY692751; AAT92770.1; -; Genomic_DNA.
DR   PIR; S54507; SYBYHM.
DR   RefSeq; NP_015358.1; -.
DR   DIP; DIP:3909N; -.
DR   IntAct; P07263; 8.
DR   PeptideAtlas; P07263; -.
DR   Ensembl; YPR033C; Saccharomyces cerevisiae.
DR   GeneID; 856145; -.
DR   GenomeReviews; U00094_GR; YPR033C.
DR   KEGG; sce:YPR033C; -.
DR   NMPDR; fig|4932.3.peg.6493; -.
DR   CYGD; YPR033c; -.
DR   SGD; S000006237; HTS1.
DR   HOGENOM; P07263; -.
DR   LinkHub; P07263; -.
DR   NextBio; 981265; -.
DR   ArrayExpress; P07263; -.
DR   GermOnline; YPR033C; Saccharomyces cerevisiae.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0004821; F:histidine-tRNA ligase activity; IEA:InterPro.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0006427; P:histidyl-tRNA aminoacylation; IEA:InterPro.
DR   InterPro; IPR002314; aa-tRNA-synt_IIb.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR004154; Anticodon_bd.
DR   InterPro; IPR015807; His-tRNA-synth_IIa_sub.
DR   InterPro; IPR015805; His-tRNA_synth.
DR   InterPro; IPR004516; His-tRNA_synth_IIA.
DR   Gene3D; G3DSA:3.40.50.800; Anticodon_bd; 1.
DR   PANTHER; PTHR11476; His-tRNA_synth; 1.
DR   Pfam; PF03129; HGTP_anticodon; 1.
DR   Pfam; PF00587; tRNA-synt_2b; 1.
DR   PIRSF; PIRSF001549; His-tRNA_synth; 1.
DR   TIGRFAMs; TIGR00442; hisS; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   1: Evidence at protein level;
KW   Alternative initiation; Aminoacyl-tRNA synthetase; ATP-binding;
KW   Complete proteome; Cytoplasm; Ligase; Mitochondrion;
KW   Nucleotide-binding; Protein biosynthesis; Transit peptide.
FT   TRANSIT       1     20       Mitochondrion.
FT   CHAIN        21    546       Histidyl-tRNA synthetase, mitochondrial.
FT                                /FTId=PRO_0000035804.
FT   VAR_SEQ       1     20       Missing (in isoform Cytoplasmic).
FT                                /FTId=VSP_018907.
FT   CONFLICT    476    478       AAE -> TTK (in Ref. 1; AAA34695/
FT                                AAA34696).
SQ   SEQUENCE   546 AA;  59953 MW;  8BDCCEAB4DD73973 CRC64;
     MLSRSLNKVV TSIKSSSIIR MSSATAAATS APTANAANAL KASKAPKKGK LQVSLKTPKG
     TKDWADSDMV IREAIFSTLS GLFKKHGGVT IDTPVFELRE ILAGKYGEDS KLIYNLEDQG
     GELCSLRYDL TVPFARYVAM NNIQSIKRYH IAKVYRRDQP AMTKGRMREF YQCDFDVAGT
     FESMVPDSEC LSILVEGLTS LGIKDFKIKL NHRKILDGIF QIAGVKDEDV RKISSAVDKL
     DKSPWEAVKK EMTEEKGQSE ETADKIGEYV KLNGSLKEIH AVLSADANIT SNEKAKQGLD
     DIATLMKYTE AFDIDSFISF DLSLARGLDY YTGLIYEVVT SASAPPENAS ELKKKAKSAE
     DASEFVGVGS IAAGGRYDNL VNMFSEASGK KSTQIPCVGI SFGVERIFSL IKQRINSSTT
     IKPTATQVFV MAFGGGKDWT GYLPERMKVT KQLWDAGIEA EYVYKAKANP RKQFDAAEKA
     GCHIAVILGK EEYLEGKLRV KRLGQEFADD DGELVSAADI VPIVQEKLSQ IHEDGLNEVT
     RLIKGL
//