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UniProtKB/Swiss-Prot entry P07263


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name SYH_YEAST
Primary accession number P07263
Secondary accession number Q12095
Integrated into Swiss-Prot on April 1, 1988
Sequence was last modified on November 1, 1997 (Sequence version 2)
Annotations were last modified on    November 4, 2008 (Entry version 97)
Name and origin of the protein
Protein name Histidyl-tRNA synthetase, mitochondrial [Precursor]
Synonyms EC 6.1.1.21
Histidine--tRNA ligase
HisRS
Gene name
Name: HTS1
OrderedLocusNames: YPR033C
ORFNames: YP9367.13C
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0092-8674(86)90740-3; PubMed=3521891 [NCBI, ExPASy, EBI, Israel, Japan]
Natsoulis G., Hilger F., Fink G.R.;
"The HTS1 gene encodes both the cytoplasmic and mitochondrial histidine tRNA synthetases of S. cerevisiae.";
Cell 46:235-243(1986).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204511 / S288c / AB972;
PubMed=9169875 [NCBI, ExPASy, EBI, Israel, Japan]
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
Nature 387:103-105(1997).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
DOI=10.1101/gr.6037607; PubMed=17322287 [NCBI, ExPASy, EBI, Israel, Japan]
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.;
"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.";
Genome Res. 17:536-543(2007).
[4]
MUTAGENESIS.
PubMed=1459448 [NCBI, ExPASy, EBI, Israel, Japan]
Chiu M.I., Mason T.L., Fink G.R.;
"HTS1 encodes both the cytoplasmic and mitochondrial histidyl-tRNA synthetase of Saccharomyces cerevisiae: mutations alter the specificity of compartmentation.";
Genetics 132:987-1001(1992).
[5]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M14048; AAA34695.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M14048; AAA34696.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z71255; CAA94983.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z49274; CAA89287.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY692751; AAT92770.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S54507; SYBYHM.
RefSeq NP_015358.1; -.
3D structure databases
ModBase P07263.
Protein-protein interaction databases
DIP DIP:3909N; -.
IntAct P07263; -.
Organism-specific databases
CYGD YPR033c; -.
SGD S000006237; HTS1.
Yeast-GFP YPR033C.
Gene expression databases
ArrayExpress P07263; -.
GermOnline YPR033C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005739; Cellular component: mitochondrion (inferred from mutant phenotype from SGD).
GO:0004821; Molecular function: histidine-tRNA ligase activity (traceable author statement from SGD).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0006427; Biological process: histidyl-tRNA aminoacylation (inferred from mutant phenotype from SGD).
QuickGo view.
Family and domain databases
InterPro IPR002314; aa-tRNA-synt_IIb.
IPR006195; aa-tRNA-synth_II.
IPR004154; Anticodon_bd.
IPR015807; His-tRNA-synth_IIa_sub.
IPR015805; His-tRNA_synth.
IPR004516; His-tRNA_synth_IIA.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.800; Anticodon_bd; 1.
PANTHER PTHR11476; His-tRNA_synth; 1.
Pfam PF03129; HGTP_anticodon; 1.
PF00587; tRNA-synt_2b; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF001549; His-tRNA_synth; 1.
TIGRFAMs TIGR00442; hisS; 1.
PROSITE PS50862; AA_TRNA_LIGASE_II; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P07263.
ProtoNet P07263.
Proteomic databases
PeptideAtlas P07263; -.
Genome annotation databases
Ensembl YPR033C; Saccharomyces cerevisiae. [Contig view]
GeneID 856145; -.
GenomeReviews U00094_GR; YPR033C.
KEGG sce:YPR033C; -.
NMPDR fig|4932.3.peg.6493; -.
Phylogenomic databases
HOGENOM P07263; -.
Other
LinkHub P07263; -.
NextBio 981265; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative initiation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Mitochondrion; Nucleotide-binding; Protein biosynthesis; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    20  20     Mitochondrion. 
CHAIN   21   546  526     Histidyl-tRNA synthetase, mitochondrial. PRO_0000035804
VAR_SEQ   1    20        Missing (in isoform Cytoplasmic). VSP_018907
CONFLICT   476   478        AAE -> TTK (in Ref. 1; AAA34695/AAA34696). 
Sequence information
Length: 546 AA [This is the length of the unprocessed precursor] Molecular weight: 59953 Da [This is the MW of the unprocessed precursor] CRC64: 8BDCCEAB4DD73973 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MLSRSLNKVV TSIKSSSIIR MSSATAAATS APTANAANAL KASKAPKKGK LQVSLKTPKG 

        70         80         90        100        110        120 
TKDWADSDMV IREAIFSTLS GLFKKHGGVT IDTPVFELRE ILAGKYGEDS KLIYNLEDQG 

       130        140        150        160        170        180 
GELCSLRYDL TVPFARYVAM NNIQSIKRYH IAKVYRRDQP AMTKGRMREF YQCDFDVAGT 

       190        200        210        220        230        240 
FESMVPDSEC LSILVEGLTS LGIKDFKIKL NHRKILDGIF QIAGVKDEDV RKISSAVDKL 

       250        260        270        280        290        300 
DKSPWEAVKK EMTEEKGQSE ETADKIGEYV KLNGSLKEIH AVLSADANIT SNEKAKQGLD 

       310        320        330        340        350        360 
DIATLMKYTE AFDIDSFISF DLSLARGLDY YTGLIYEVVT SASAPPENAS ELKKKAKSAE 

       370        380        390        400        410        420 
DASEFVGVGS IAAGGRYDNL VNMFSEASGK KSTQIPCVGI SFGVERIFSL IKQRINSSTT 

       430        440        450        460        470        480 
IKPTATQVFV MAFGGGKDWT GYLPERMKVT KQLWDAGIEA EYVYKAKANP RKQFDAAEKA 

       490        500        510        520        530        540 
GCHIAVILGK EEYLEGKLRV KRLGQEFADD DGELVSAADI VPIVQEKLSQ IHEDGLNEVT 


RLIKGL 

P07263 in FASTA format

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