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UniProtKB/Swiss-Prot entry P07199


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CENPB_HUMAN
Primary accession number P07199
Secondary accession numbers None
Integrated into Swiss-Prot on April 1, 1988
Sequence was last modified on August 1, 1992 (Sequence version 2)
Annotations were last modified on    June 16, 2009 (Entry version 109)
Name and origin of the protein
Protein name Major centromere autoantigen B
Synonyms Centromere protein B
CENP-B
Gene name
Name: CENPB
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1007/BF00337514; PubMed=1893793 [NCBI, ExPASy, EBI, Israel, Japan]
Sullivan K.F., Glass C.A.;
"CENP-B is a highly conserved mammalian centromere protein with homology to the helix-loop-helix family of proteins.";
Chromosoma 100:360-370(1991).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/414865a; PubMed=11780052 [NCBI, ExPASy, EBI, Israel, Japan]
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.;
"The DNA sequence and comparative analysis of human chromosome 20.";
Nature 414:865-871(2001).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Uterus;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[4]
NUCLEOTIDE SEQUENCE [MRNA] OF 6-599.
DOI=10.1083/jcb.104.4.817; PubMed=2435739 [NCBI, ExPASy, EBI, Israel, Japan]
Earnshaw W.C., Sullivan K.F., Machlin P.S., Cooke C.A., Kaiser D.A., Pollard T.D., Rothfield N.F., Cleveland D.W.;
"Molecular cloning of cDNA for CENP-B, the major human centromere autoantigen.";
J. Cell Biol. 104:817-829(1987).
[5]
SUBUNIT, AND DOMAINS.
DOI=10.1083/jcb.119.6.1413; PubMed=1469042 [NCBI, ExPASy, EBI, Israel, Japan]
Yoda K., Kitagawa K., Masumoto H., Muro Y., Okazaki T.;
"A human centromere protein, CENP-B, has a DNA binding domain containing four potential alpha helices at the NH2 terminus, which is separable from dimerizing activity.";
J. Cell Biol. 119:1413-1427(1992).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-156 AND SER-165, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1073/pnas.0507066103; PubMed=16565220 [NCBI, ExPASy, EBI, Israel, Japan]
Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R.;
"Phosphoproteome analysis of the human mitotic spindle.";
Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-156, AND MASS SPECTROMETRY.
DOI=10.1021/pr0705441; PubMed=18220336 [NCBI, ExPASy, EBI, Israel, Japan]
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III;
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.";
J. Proteome Res. 7:1346-1351(2008).
[8]
STRUCTURE BY NMR OF 1-56.
DOI=10.1093/emboj/17.3.827; PubMed=9451007 [NCBI, ExPASy, EBI, Israel, Japan]
Iwahara J., Kigawa T., Kitagawa K., Masumoto H., Okazaki T., Yokoyama S.;
"A helix-turn-helix structure unit in human centromere protein B (CENP-B).";
EMBO J. 17:827-837(1998).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X55039; CAA38879.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL109804; CAC17547.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC053847; AAH53847.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X05299; CAA28918.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00010388; -.
PIR S18735; S18735.
RefSeq NP_001801.1; -.
UniGene Hs.516855
3D structure databases
PDB
1BW6; NMR; -; A=1-56.[ExPASy / RCSB / EBI]
1HLV; X-ray; 2.50 A; A=1-129.[ExPASy / RCSB / EBI]
1UFI; X-ray; 1.65 A; A/B/C/D=540-599.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1BW6; -.
1HLV; -.
1UFI; -.
SMR P07199; 539-585.
ModBase P07199.
Protein-protein interaction databases
IntAct P07199; 3.
PTM databases
PhosphoSite P07199; -.
Enzyme and pathway databases
Pathway_Interaction_DB foxm1pathway; FOXM1 transcription factor network.
Organism-specific databases
GeneCards GC20M003712; -.
H-InvDB HIX0027657; -.
HGNC HGNC:1852; CENPB.
GenAtlas CENPB.
HPA CAB011626; -.
MIM 117140; gene. [NCBI / EBI]
PharmGKB PA26397; -.
Gene expression databases
ArrayExpress P07199; -.
Bgee P07199; -.
CleanEx HS_CENPB; -.
GermOnline ENSG00000125817; Homo sapiens.
Ontologies
GO
GO:0000775; Cellular component: chromosome, centromeric region (non-traceable author statement from UniProtKB).
GO:0005634; Cellular component: nucleus (inferred from electronic annotation from UniProtKB-SubCell).
GO:0003682; Molecular function: chromatin binding (non-traceable author statement from UniProtKB).
GO:0003696; Molecular function: satellite DNA binding (non-traceable author statement from UniProtKB).
GO:0045449; Biological process: regulation of transcription (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR015115; Centromere_CenpB_dimerisation.
IPR006695; Centromere_CenpB_DNA-db_1.
IPR015175; Centromere_CenpB_DNA-db_2.
IPR006600; Centromere_CenpB_HTH.
IPR004875; DDE_SF_endonuclease_CENPB-like.
IPR012287; Homeodomain-rel.
IPR011526; HTH_psq-like.
Graphical view of domain structure.
Gene3D G3DSA:1.10.10.60; Homeodomain-rel; 2.
Pfam PF09026; Cenp-B_dimeris; 1.
PF04218; CENP-B_N; 1.
PF09091; CenpB-DNA-bind; 1.
PF03184; DDE; 1.
Pfam graphical view of domain structure.
SMART SM00674; CENPB; 1.
SMART graphical view of domain structure.
PROSITE PS51253; HTH_CENPB; 1.
PS50960; HTH_PSQ; 1.
PROSITE graphical view of domain structure (profiles).
Proteomic databases
PeptideAtlas P07199; -.
PRIDE P07199; -.
Genome annotation databases
Ensembl ENSG00000125817; Homo sapiens. [Contig view]
GeneID 1059; -.
KEGG hsa:1059; -.
Phylogenomic databases
HOGENOM P07199; -.
HOVERGEN P07199; -.
OMA P07199; TASNGWL.
Other
NextBio 4432; -.
SOURCE CENPB; Homo sapiens.
ProtoNet P07199.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Centromere; Chromosomal protein; DNA-binding; Nucleus; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   599  599     Major centromere autoantigen B. PRO_0000126125
DOMAIN   1    52  52     HTH psq-type. 
DOMAIN   65   136  72     HTH CENPB-type. 
DNA_BIND   28    48  21     H-T-H motif. 
DNA_BIND   97   129  33     H-T-H motif. 
COMPBIAS   404   465  62     Glu-rich (acidic). 
COMPBIAS   508   538  31     Asp/Glu-rich (acidic). 
MOD_RES   156   156        Phosphoserine. 
MOD_RES   165   165        Phosphoserine. 
CONFLICT   583   583        R -> M (in Ref. 4; CAA28918). 
CONFLICT   592   593        VR -> LL (in Ref. 4; CAA28918). 
HELIX   10    22  13      
HELIX   28    35  8      
HELIX   39    47  9      
HELIX   49    59  11      
HELIX   60    64  5      
HELIX   75    88  14      
HELIX   89    91  3      
HELIX   97   111  15      
HELIX   544   558  15      
HELIX   565   584  20      
Sequence information
Length: 599 AA [This is the length of the unprocessed precursor] Molecular weight: 65171 Da [This is the MW of the unprocessed precursor] CRC64: 9B4B7DB957A914AA [This is a checksum on the sequence]
        10         20         30         40         50         60 
MGPKRRQLTF REKSRIIQEV EENPDLRKGE IARRFNIPPS TLSTILKNKR AILASERKYG 

        70         80         90        100        110        120 
VASTCRKTNK LSPYDKLEGL LIAWFQQIRA AGLPVKGIIL KEKALRIAEE LGMDDFTASN 

       130        140        150        160        170        180 
GWLDRFRRRH GVVSCSGVAR ARARNAAPRT PAAPASPAAV PSEGSGGSTT GWRAREEQPP 

       190        200        210        220        230        240 
SVAEGYASQD VFSATETSLW YDFLPDQAAG LCGGDGRPRQ ATQRLSVLLC ANADGSEKLP 

       250        260        270        280        290        300 
PLVAGKSAKP RAGQAGLPCD YTANSKGGVT TQALAKYLKA LDTRMAAESR RVLLLAGRLA 

       310        320        330        340        350        360 
AQSLDTSGLR HVQLAFFPPG TVHPLERGVV QQVKGHYRQA MLLKAMAALE GQDPSGLQLG 

       370        380        390        400        410        420 
LTEALHFVAA AWQAVEPSDI AACFREAGFG GGPNATITTS LKSEGEEEEE EEEEEEEEEG 

       430        440        450        460        470        480 
EGEEEEEEGE EEEEEGGEGE ELGEEEEVEE EGDVDSDEEE EEDEESSSEG LEAEDWAQGV 

       490        500        510        520        530        540 
VEAGGSFGAY GAQEEAQCPT LHFLEGGEDS DSDSEEEDDE EEDDEDEDDD DDEEDGDEVP 

       550        560        570        580        590 
VPSFGEAMAY FAMVKRYLTS FPIDDRVQSH ILHLEHDLVH VTRKNHARQA GVRGLGHQS 

P07199 in FASTA format

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