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UniProtKB/Swiss-Prot entry P07014


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DHSB_ECOLI
Primary accession number P07014
Secondary accession numbers None
Integrated into Swiss-Prot on April 1, 1988
Sequence was last modified on April 1, 1988 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 91)
Name and origin of the protein
Protein name Succinate dehydrogenase iron-sulfur subunit
Synonym EC 1.3.99.1
Gene name
Name: sdhB
OrderedLocusNames: b0724, JW0714
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=K12;
PubMed=6388571 [NCBI, ExPASy, EBI, Israel, Japan]
Darlison M.G., Guest J.R.;
"Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase of Escherichia coli.";
Biochem. J. 223:507-517(1984).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1093/dnares/3.3.137; PubMed=8905232 [NCBI, ExPASy, EBI, Israel, Japan]
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.;
"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.";
DNA Res. 3:137-155(1996).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[5]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 231-238.
PubMed=6376123 [NCBI, ExPASy, EBI, Israel, Japan]
Darlison M.G., Spencer M.E., Guest J.R.;
"Nucleotide sequence of the sucA gene encoding the 2-oxoglutarate dehydrogenase of Escherichia coli K12.";
Eur. J. Biochem. 141:351-359(1984).
[6]
PROTEIN SEQUENCE OF 1-11.
STRAIN=K12 / EMG2;
PubMed=9298646 [NCBI, ExPASy, EBI, Israel, Japan]
Link A.J., Robison K., Church G.M.;
"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.";
Electrophoresis 18:1259-1313(1997).
[7]
SUBUNIT, AND SUBCELLULAR LOCATION.
STRAIN=BL21-DE3;
DOI=10.1074/jbc.M506479200; PubMed=16079137 [NCBI, ExPASy, EBI, Israel, Japan]
Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L., von Heijne G., Daley D.O.;
"Protein complexes of the Escherichia coli cell envelope.";
J. Biol. Chem. 280:34409-34419(2005).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
J01619; AAA23896.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X01070; CAA25534.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC73818.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAA35391.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X00661; CAA25279.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A28837; DEECSI.
RefSeq AP_001362.1; -.
NP_415252.1; -.
3D structure databases
PDB
1NEK; X-ray; 2.60 A; B=1-238.[ExPASy / RCSB / EBI]
1NEN; X-ray; 2.90 A; B=1-238.[ExPASy / RCSB / EBI]
2ACZ; X-ray; 3.10 A; B=1-238.[ExPASy / RCSB / EBI]
2AD0; Model; -; B=1-238.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1NEK; -.
1NEN; -.
2ACZ; -.
2AD0; -.
ModBase P07014.
Protein-protein interaction databases
DIP DIP:10836N; -.
IntAct P07014; -.
Enzyme and pathway databases
BioCyc EcoCyc:SDH-FE-S; -.
MetaCyc:SDH-FE-S; -.
Organism-specific databases
EchoBASE EB0925; -.
EcoGene EG10932; sdhB.
Ontologies
GO
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR006058; 2Fe2S_fd_BS.
IPR001450; 4Fe4S_Fe_S_bd.
IPR012675; b-grasp_ferredoxin-like.
IPR001041; Ferredoxin.
IPR012285; Fum_reductase_C.
IPR004489; Succ_DHase/fum_Rdtase_Fe-S.
Graphical view of domain structure.
Gene3D G3DSA:3.10.20.30; Ferredoxin_fold; 1.
G3DSA:1.10.1060.10; Fum_reductase_C; 1.
TIGRFAMs TIGR00384; dhsB; 1.
PROSITE PS00197; 2FE2S_FER_1; FALSE_NEG.
PS51085; 2FE2S_FER_2; 1.
PS00198; 4FE4S_FER_1; 1.
PS51379; 4FE4S_FER_2; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P07014.
Genome annotation databases
GeneID 945300; -.
GenomeReviews U00096_GR; b0724.
AP009048_GR; JW0714.
KEGG ecj:JW0714; -.
eco:b0724; -.
Phylogenomic databases
HOGENOM P07014; -.
Genome annotation databases
CMR P07014; b0724.
Other
ProtoNet P07014.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
2Fe-2S; 3D-structure; 3Fe-4S; 4Fe-4S; Cell inner membrane; Cell membrane; Complete proteome; Direct protein sequencing; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Oxidoreductase; Transport; Tricarboxylic acid cycle.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   238  238     Succinate dehydrogenase iron-sulfur subunit. PRO_0000158688
DOMAIN   8    97  90     2Fe-2S ferredoxin-type. 
DOMAIN   139   169  31     4Fe-4S ferredoxin-type. 
METAL   55    55        Iron-sulfur 1 (2Fe-2S) (By similarity). 
METAL   60    60        Iron-sulfur 1 (2Fe-2S) (By similarity). 
METAL   63    63        Iron-sulfur 1 (2Fe-2S) (Potential). 
METAL   75    75        Iron-sulfur 1 (2Fe-2S) (By similarity). 
METAL   149   149        Iron-sulfur 2 (4Fe-4S) (By similarity). 
METAL   152   152        Iron-sulfur 2 (4Fe-4S) (By similarity). 
METAL   155   155        Iron-sulfur 2 (4Fe-4S) (By similarity). 
METAL   159   159        Iron-sulfur 3 (3Fe-4S) (By similarity). 
METAL   206   206        Iron-sulfur 3 (3Fe-4S) (By similarity). 
METAL   212   212        Iron-sulfur 3 (3Fe-4S) (By similarity). 
METAL   216   216        Iron-sulfur 2 (4Fe-4S) (By similarity). 
STRAND   4     9  6      
TURN   12    14  3      
STRAND   19    24  6      
STRAND   29    31  3      
HELIX   35    45  11      
STRAND   55    58  4      
STRAND   64    67  4      
STRAND   70    73  4      
TURN   79    82  4      
STRAND   89    92  4      
STRAND   95   101  7      
HELIX   108   116  9      
STRAND   130   132  3      
HELIX   137   141  5      
TURN   142   146  5      
HELIX   154   157  4      
HELIX   160   164  5      
TURN   166   168  3      
HELIX   172   182  11      
HELIX   190   195  6      
STRAND   199   201  3      
TURN   202   205  4      
HELIX   211   215  5      
HELIX   222   236  15      
Sequence information
Length: 238 AA [This is the length of the unprocessed precursor] Molecular weight: 26770 Da [This is the MW of the unprocessed precursor] CRC64: 226F60C55F5AC35A [This is a checksum on the sequence]
        10         20         30         40         50         60 
MRLEFSIYRY NPDVDDAPRM QDYTLEADEG RDMMLLDALI QLKEKDPSLS FRRSCREGVC 

        70         80         90        100        110        120 
GSDGLNMNGK NGLACITPIS ALNQPGKKIV IRPLPGLPVI RDLVVDMGQF YAQYEKIKPY 

       130        140        150        160        170        180 
LLNNGQNPPA REHLQMPEQR EKLDGLYECI LCACCSTSCP SFWWNPDKFI GPAGLLAAYR 

       190        200        210        220        230 
FLIDSRDTET DSRLDGLSDA FSVFRCHSIM NCVSVCPKGL NPTRAIGHIK SMLLQRNA 

P07014 in FASTA format

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