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UniProtKB/Swiss-Prot entry P06999


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name K6PF2_ECOLI
Primary accession number P06999
Secondary accession numbers P78065 P78260
Integrated into Swiss-Prot on April 1, 1988
Sequence was last modified on November 1, 1997 (Sequence version 2)
Annotations were last modified on    November 4, 2008 (Entry version 79)
Name and origin of the protein
Protein name 6-phosphofructokinase isozyme 2
Synonyms Phosphofructokinase-2
EC 2.7.1.11
Gene name
Name: pfkB
OrderedLocusNames: b1723, JW5280
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=K12;
DOI=10.1016/0378-1119(84)90151-3; PubMed=6235149 [NCBI, ExPASy, EBI, Israel, Japan]
Daldal F.;
"Nucleotide sequence of gene pfkB encoding the minor phosphofructokinase of Escherichia coli K-12.";
Gene 28:337-342(1984).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1093/dnares/3.6.363; PubMed=9097039 [NCBI, ExPASy, EBI, Israel, Japan]
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.;
"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.";
DNA Res. 3:363-377(1996).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[5]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-38.
DOI=10.1016/S0022-2836(83)80019-9; PubMed=6310120 [NCBI, ExPASy, EBI, Israel, Japan]
Daldal F.;
"Molecular cloning of the gene for phosphofructokinase-2 of Escherichia coli and the nature of a mutation, pfkB1, causing a high level of the enzyme.";
J. Mol. Biol. 168:285-305(1983).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
K02500; AAA24321.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC74793.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAA15500.2; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
K00128; AAA24320.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR C64931; KIECFB.
RefSeq AP_002342.1; -.
NP_416237.3; -.
3D structure databases
ModBase P06999.
Protein-protein interaction databases
DIP DIP:10465N; -.
Enzyme and pathway databases
BioCyc EcoCyc:6PFK-2-MON; -.
MetaCyc:6PFK-2-MON; -.
2D gel databases
SWISS-2DPAGE P06999; -.
2DBase-Ecoli P06999; -.
ECO2DBASE E036.6; 6TH EDITION.
Organism-specific databases
EchoBASE EB0694; -.
EcoGene EG10700; pfkB.
Ontologies
GO
GO:0003872; Molecular function: 6-phosphofructokinase activity (inferred from electronic annotation from EC).
GO:0006096; Biological process: glycolysis (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR017583; 1-phosphofructokinase.
IPR011611; Carb/pur_kinase.
IPR002173; Carboh/pur_kinase_PfkB_CS.
Graphical view of domain structure.
Pfam PF00294; PfkB; 1.
Pfam graphical view of domain structure.
PROSITE PS00583; PFKB_KINASES_1; 1.
PS00584; PFKB_KINASES_2; 1.
BLOCKS P06999.
ProtoNet P06999.
Genome annotation databases
GeneID 946230; -.
GenomeReviews U00096_GR; b1723.
AP009048_GR; JW5280.
KEGG ecj:JW5280; -.
eco:b1723; -.
Phylogenomic databases
HOGENOM P06999; -.
Genome annotation databases
CMR P06999; b1723.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Glycolysis; Kinase; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   309  309     6-phosphofructokinase isozyme 2. PRO_0000080083
CONFLICT   26    38        GKLRCTAPVFEPG -> ENCAVPHRCSNP (in Ref. 1 and 5). 
CONFLICT   155   171        AAQKQGIRCIVDSSGEA -> LRKNKGSAASSTVLGQG (in Ref. 1; AAA24320). 
CONFLICT   245   246        PV -> AL (in Ref. 1; AAA24321). 
CONFLICT   257   258        SM -> RL (in Ref. 1; AAA24321). 
Sequence information
Length: 309 AA [This is the length of the unprocessed precursor] Molecular weight: 32456 Da [This is the MW of the unprocessed precursor] CRC64: A93BEBE0D5801309 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MVRIYTLTLA PSLDSATITP QIYPEGKLRC TAPVFEPGGG GINVARAIAH LGGSATAIFP 

        70         80         90        100        110        120 
AGGATGEHLV SLLADENVPV ATVEAKDWTR QNLHVHVEAS GEQYRFVMPG AALNEDEFRQ 

       130        140        150        160        170        180 
LEEQVLEIES GAILVISGSL PPGVKLEKLT QLISAAQKQG IRCIVDSSGE ALSAALAIGN 

       190        200        210        220        230        240 
IELVKPNQKE LSALVNRELT QPDDVRKAAQ EIVNSGKAKR VVVSLGPQGA LGVDSENCIQ 

       250        260        270        280        290        300 
VVPPPVKSQS TVGAGDSMVG AMTLKLAENA SLEEMVRFGV AAGSAATLNQ GTRLCSHDDT 


QKIYAYLSR 

P06999 in FASTA format

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View entry in raw text format (no links)
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