[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 230-243. PubMed=2993245 [NCBI, ExPASy, EBI, Israel, Japan] Takagi M., Imanaka T., Aiba S.; "Nucleotide sequence and promoter region for the neutral protease gene from Bacillus stearothermophilus."; J. Bacteriol. 163:824-831(1985).

Comments

FUNCTION: Extracellular zinc metalloprotease.
CATALYTIC ACTIVITY: Preferential cleavage: Xaa-|-Leu > Xaa-|-Phe.
COFACTOR: Binds 4 calcium ions per subunit.
COFACTOR: Binds 1 zinc ion per subunit.
BIOPHYSICOCHEMICAL PROPERTIES:

Temperature dependence: Thermostable;
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: Belongs to the peptidase M4 family [view classification].

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M11446; AAA22621.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A24924; HYBSS.

3D structure databases

HSSP

P00800; 1KJO. [HSSP ENTRY / PDB]

ModBase

P06874.

Protein family/group databases

MEROPS

M04.001; -.

Ontologies

GO:0005509;	Molecular function: calcium ion binding (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR005075; Pept_M4_propep_PepSY.
IPR006025; Pept_M_Zn_BS.
IPR013856; Peptidase_M4.
IPR001570; Peptidase_M4_C.
IPR011096; Propep_M4_M36.
Graphical view of domain structure.

Gene3D

G3DSA:3.10.170.10; Peptidase_M4; 1.
G3DSA:1.10.390.10; Peptidase_M4/M36; 1.

Pfam

PF07504; FTP; 1.
PF03413; PepSY; 1.
PF01447; Peptidase_M4; 1.
PF02868; Peptidase_M4_C; 1.
Pfam graphical view of domain structure.

PRINTS

PR00730; THERMOLYSIN.

PROSITE

PS00142; ZINC_PROTEASE; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calcium; Direct protein sequencing; Hydrolase; Metal-binding; Metalloprotease; Protease; Secreted; Signal; Zinc; Zymogen.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 25`	`25`
`PROPEP`	`26 229`	`204`	`Activation peptide.`	`PRO_0000028590`
`CHAIN`	`230 548`	`319`	`Thermolysin.`	`PRO_0000028591`
`ACT_SITE`	`375 375`
`ACT_SITE`	`463 463`		`Proton donor.`
`METAL`	`289 289`		`Calcium 1 (By similarity).`
`METAL`	`291 291`		`Calcium 1 (By similarity).`
`METAL`	`293 293`		`Calcium 1; via carbonyl oxygen (By similarity).`
`METAL`	`370 370`		`Calcium 2 (By similarity).`
`METAL`	`374 374`		`Zinc; catalytic.`
`METAL`	`378 378`		`Zinc; catalytic.`
`METAL`	`398 398`		`Zinc; catalytic.`
`METAL`	`409 409`		`Calcium 2 (By similarity).`
`METAL`	`409 409`		`Calcium 3 (By similarity).`
`METAL`	`415 415`		`Calcium 3; via carbonyl oxygen (By similarity).`
`METAL`	`417 417`		`Calcium 2 (By similarity).`
`METAL`	`417 417`		`Calcium 3 (By similarity).`
`METAL`	`419 419`		`Calcium 2; via carbonyl oxygen (By similarity).`
`METAL`	`422 422`		`Calcium 2 (By similarity).`
`METAL`	`422 422`		`Calcium 3 (By similarity).`
`METAL`	`425 425`		`Calcium 4; via carbonyl oxygen (By similarity).`
`METAL`	`426 426`		`Calcium 4 (By similarity).`
`METAL`	`429 429`		`Calcium 4; via carbonyl oxygen (By similarity).`
`METAL`	`432 432`		`Calcium 4 (By similarity).`

Sequence information

Length: 548 AA [This is the length of the unprocessed precursor]

Molecular weight: 59580 Da [This is the MW of the unprocessed precursor]

CRC64: 5B4D2C1D7910539D [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNKRAMLGAI GLAFGLLAAP IGASAKGESI VWNEQWKTPS FVSGSLLNGG EQALEELVYQ 

        70         80         90        100        110        120 
YVDRENGTFR LGGRARDRLA LIGKQTDELG HTVMRFEQRH HGIPVYGTML AAHVKDGELI 

       130        140        150        160        170        180 
ALSGSLIPNL DGQPRLKKAK TVTVQQAEAI AEQDVTETVT KERPTTENGE RTRLVIYPTD 

       190        200        210        220        230        240 
GTARLAYEVN VRFLTPVPGN WVYIIDATDG AILNKFNQID SRQPGGGQPV AGASTVGVGR 

       250        260        270        280        290        300 
GVLGDQKYIN TTYSSYYGYY YLQDNTRGSG IFTYDGRNRT VLPGSLWTDG DNQFTASYDA 

       310        320        330        340        350        360 
AAVDAHYYAG VVYDYYKNVH GRLSYDGSNA AIRSTVHYGR GYNNAFWNGS QMVYGDGDGQ 

       370        380        390        400        410        420 
TFLPFSGGID VVGHELTHAV TDYTAGLVYQ NESGAINEAM SDIFGTLVEF YANRNPDWEI 

       430        440        450        460        470        480 
GEDIYTPGVA GDALRSMSDP AKYGDPDHYS KRYTGTQDNG GVHTNSGIIN KAAYLLSQGG 

       490        500        510        520        530        540 
VHYGVSVNGI GRDKMGKIFY RALVYYLTPT SNFSQLRAAC VQAAADLYGS TSQEVNSVKQ 


AFNAVGVY

P06874 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools