Urokinase-type plasminogen activator long chain A
Urokinase-type plasminogen activator short chain A
Urokinase-type plasminogen activator chain B

Gene name

Name:

Plau

From

Mus musculus (Mouse)

[TaxID: 10090]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.

Protein existence

2: Evidence at transcript level;

References

[1]	NUCLEOTIDE SEQUENCE [MRNA]. PubMed=2985383 [NCBI, ExPASy, EBI, Israel, Japan] Belin D., Vassalli J.-D., Combepine C., Godeau F., Nagamine Y., Reich E., Kocher H.P., Duvoisin R.M.; "Cloning, nucleotide sequencing and expression of cDNAs encoding mouse urokinase-type plasminogen activator."; Eur. J. Biochem. 148:225-232(1985).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. DOI=10.1021/bi00399a038; PubMed=2831940 [NCBI, ExPASy, EBI, Israel, Japan] Degen S.J.F., Heckel J.L., Reich E., Degen J.L.; "The murine urokinase-type plasminogen activator gene."; Biochemistry 26:8270-8279(1987).

Comments

FUNCTION: Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
CATALYTIC ACTIVITY: Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
SUBUNIT: Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR (By similarity).
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: Belongs to the peptidase S1 family [view classification].
SIMILARITY: Contains 1 EGF-like domain.
SIMILARITY: Contains 1 kringle domain.
SIMILARITY: Contains 1 peptidase S1 domain [view classification].

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X02389; CAA26231.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M17922; AAA40539.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

A29420; UKMS.

NP_032899.1; -.

3D structure databases

HSSP

P00749; 1KDU. [HSSP ENTRY / PDB]

SMR

P06869; 30-153, 180-428.

ModBase

P06869.

Protein family/group databases

MEROPS

S01.231; -.

PTM databases

PhosphoSite

P06869; -.

Organism-specific databases

MGI

MGI:97611; Plau.

Gene expression databases

ArrayExpress

P06869; -.

CleanEx

MM_PLAU; -.

GermOnline

ENSMUSG00000021822; Mus musculus.

Ontologies

GO:0001666;	Biological process: response to hypoxia (inferred from mutant phenotype from MGI).
GO:0014909;	Biological process: smooth muscle cell migration (inferred from mutant phenotype from MGI).
QuickGo view.

Family and domain databases

InterPro

IPR006210; EGF.
IPR000742; EGF_3.
IPR013111; EGF_extracell.
IPR013032; EGF_like_reg_CS.
IPR000001; Kringle.
IPR001254; Peptidase_S1_S6.
IPR001314; Peptidase_S1A.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.20.10; Kringle; 1.

Pfam

PF07974; EGF_2; 1.
PF00051; Kringle; 1.
PF00089; Trypsin; 1.
Pfam graphical view of domain structure.

PRINTS

PR00722; CHYMOTRYPSIN.
PR00018; KRINGLE.

ProDom

PD000395; Kringle; 1.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00181; EGF; 1.
SM00130; KR; 1.
SM00020; Tryp_SPc; 1.
SMART graphical view of domain structure.

PROSITE

PS00022; EGF_1; 1.
PS01186; EGF_2; FALSE_NEG.
PS50026; EGF_3; 1.
PS00021; KRINGLE_1; 1.
PS50070; KRINGLE_2; 1.
PS50240; TRYPSIN_DOM; 1.
PS00134; TRYPSIN_HIS; FALSE_NEG.
PS00135; TRYPSIN_SER; 1.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

Ensembl

ENSMUSG00000021822; Mus musculus. [Contig view]

GeneID

18792; -.

KEGG

mmu:18792; -.

Phylogenomic databases

HOGENOM

P06869; -.

HOVERGEN

P06869; -.

Other

NextBio

295072; -.

SOURCE

Plau; Mus musculus.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

EGF-like domain; Hydrolase; Kringle; Plasminogen activation; Protease; Secreted; Serine protease; Signal; Zymogen.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 20`	`20`	`Potential.`
`CHAIN`	`21 433`	`413`	`Urokinase-type plasminogen activator.`	`PRO_0000028322`
`CHAIN`	`21 178`	`158`	`Urokinase-type plasminogen activator long chain A (By similarity).`	`PRO_0000028323`
`CHAIN`	`157 178`	`22`	`Urokinase-type plasminogen activator short chain A (By similarity).`	`PRO_0000028324`
`CHAIN`	`180 433`	`254`	`Urokinase-type plasminogen activator chain B (By similarity).`	`PRO_0000028325`
`DOMAIN`	`28 64`	`37`	`EGF-like.`
`DOMAIN`	`71 152`	`82`	`Kringle.`
`DOMAIN`	`180 426`	`247`	`Peptidase S1.`
`REGION`	`35 58`	`24`	`Binds urokinase plasminogen activator surface receptor (By similarity).`
`REGION`	`153 179`	`27`	`Connecting peptide.`
`ACT_SITE`	`226 226`		`Charge relay system.`
`ACT_SITE`	`277 277`		`Charge relay system.`
`ACT_SITE`	`378 378`		`Charge relay system.`
`DISULFID`	`32 40`		`By similarity.`
`DISULFID`	`34 52`		`By similarity.`
`DISULFID`	`54 63`		`By similarity.`
`DISULFID`	`71 152`		`By similarity.`
`DISULFID`	`92 134`		`By similarity.`
`DISULFID`	`123 147`		`By similarity.`
`DISULFID`	`169 301`		`Interchain (between A and B chains) (By similarity).`
`DISULFID`	`211 227`		`By similarity.`
`DISULFID`	`219 290`		`By similarity.`
`DISULFID`	`315 384`		`By similarity.`
`DISULFID`	`347 363`		`By similarity.`
`DISULFID`	`374 402`		`By similarity.`

Sequence information

Length: 433 AA [This is the length of the unprocessed precursor]

Molecular weight: 48268 Da [This is the MW of the unprocessed precursor]

CRC64: A99C35F6250443F9 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKVWLASLFL CALVVKNSEG GSVLGAPDES NCGCQNGGVC VSYKYFSRIR RCSCPRKFQG 

        70         80         90        100        110        120 
EHCEIDASKT CYHGNGDSYR GKANTDTKGR PCLAWNAPAV LQKPYNAHRP DAISLGLGKH 

       130        140        150        160        170        180 
NYCRNPDNQK RPWCYVQIGL RQFVQECMVH DCSLSKKPSS SVDQQGFQCG QKALRPRFKI 

       190        200        210        220        230        240 
VGGEFTEVEN QPWFAAIYQK NKGGSPPSFK CGGSLISPCW VASAAHCFIQ LPKKENYVVY 

       250        260        270        280        290        300 
LGQSKESSYN PGEMKFEVEQ LILHEYYRED SLAYHNDIAL LKIRTSTGQC AQPSRSIQTI 

       310        320        330        340        350        360 
CLPPRFTDAP FGSDCEITGF GKESESDYLY PKNLKMSVVK LVSHEQCMQP HYYGSEINYK 

       370        380        390        400        410        420 
MLCAADPEWK TDSCKGDSGG PLICNIEGRP TLSGIVSWGR GCAEKNKPGV YTRVSHFLDW 

       430 
IQSHIGEEKG LAF

P06869 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools