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UniProtKB/Swiss-Prot entry P06850


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CRF_HUMAN
Primary accession number P06850
Secondary accession numbers None
Integrated into Swiss-Prot on January 1, 1988
Sequence was last modified on January 1, 1988 (Sequence version 1)
Annotations were last modified on    June 16, 2009 (Entry version 92)
Name and origin of the protein
Protein name Corticoliberin [Precursor]
Synonyms Corticotropin-releasing hormone
Corticotropin-releasing factor
CRF
Gene name
Name: CRH
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=6605851 [NCBI, ExPASy, EBI, Israel, Japan]
Shibahara S., Morimoto Y., Furutani Y., Notake M., Takahashi H., Shimizu S., Horikawa S., Numa S.;
"Isolation and sequence analysis of the human corticotropin-releasing factor precursor gene.";
EMBO J. 2:775-779(1983).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1016/0303-7207(89)90128-7; PubMed=2783917 [NCBI, ExPASy, EBI, Israel, Japan]
Robinson B.G., D'Angio L.A. Jr., Pasieka K.B., Majzoub J.A.;
"Preprocorticotropin releasing hormone: cDNA sequence and in vitro processing.";
Mol. Cell. Endocrinol. 61:175-180(1989).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.;
"Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Brain;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[5]
PROTEIN SEQUENCE OF 154-186.
PubMed=3262120 [NCBI, ExPASy, EBI, Israel, Japan]
Sasaki A., Tempst P., Liotta A.S., Margioris A.N., Hood L.E., Kent S.B., Sato S., Shinkawa O., Yoshinaga K., Krieger D.T.;
"Isolation and characterization of a corticotropin-releasing hormone-like peptide from human placenta.";
J. Clin. Endocrinol. Metab. 67:768-773(1988).
[6]
STRUCTURE BY NMR OF 154-194.
DOI=10.1093/protein/6.2.149; PubMed=8386360 [NCBI, ExPASy, EBI, Israel, Japan]
Romier C., Bernassau J.-M., Cambillau C., Darbon H.;
"Solution structure of human corticotropin releasing factor by 1H NMR and distance geometry with restrained molecular dynamics.";
Protein Eng. 6:149-156(1993).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
V00571; CAA23834.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT007453; AAP36121.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC002599; AAH02599.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC011031; AAH11031.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00027839; -.
PIR A30327; A30327.
RefSeq NP_000747.1; -.
UniGene Hs.75294
3D structure databases
PDB
3EHT; X-ray; 3.40 A; B=180-194.[ExPASy / RCSB / EBI]
3EHU; X-ray; 1.96 A; C/D=175-194.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 3EHT; -.
3EHU; -.
ModBase P06850.
Organism-specific databases
GeneCards GC08M067251; -.
H-InvDB HIX0007552; -.
HGNC HGNC:2355; CRH.
GenAtlas CRH.
MIM 122560; gene+phenotype. [NCBI / EBI]
PharmGKB PA119; -.
Gene expression databases
Bgee P06850; -.
CleanEx HS_CRH; -.
GermOnline ENSG00000147571; Homo sapiens.
Ontologies
GO
GO:0005576; Cellular component: extracellular region (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005625; Cellular component: soluble fraction (traceable author statement from ProtInc).
GO:0005184; Molecular function: neuropeptide hormone activity (traceable author statement from ProtInc).
GO:0007565; Biological process: female pregnancy (traceable author statement from ProtInc).
GO:0007611; Biological process: learning or memory (traceable author statement from ProtInc).
GO:0042322; Biological process: negative regulation of circadian sleep/wake cycle, REM sleep (non-traceable author statement from UniProtKB).
GO:0007567; Biological process: parturition (traceable author statement from ProtInc).
GO:0010841; Biological process: positive regulation of circadian sleep/wake cycle, wakefulness (non-traceable author statement from UniProtKB).
GO:0051464; Biological process: positive regulation of cortisol secretion (inferred from direct assay from UniProtKB).
GO:0007165; Biological process: signal transduction (traceable author statement from ProtInc).
GO:0007268; Biological process: synaptic transmission (traceable author statement from ProtInc).
QuickGo view.
Family and domain databases
InterPro IPR018447; Corticotropin-releasing_fac_C.
IPR018446; Corticotropin-releasing_fac_CS.
IPR000187; Corticotropin-releasing_factor.
IPR003620; Urocortin_CRF.
Graphical view of domain structure.
PANTHER PTHR15035; Urocortin_CRF; 1.
Pfam PF00473; CRF; 1.
Pfam graphical view of domain structure.
PRINTS PR01612; CRFFAMILY.
ProDom PD005970; Urocortin_CRF; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00039; CRF; 1.
SMART graphical view of domain structure.
PROSITE PS00511; CRF; 1.
Proteomic databases
PRIDE P06850; -.
Genome annotation databases
Ensembl ENSG00000147571; Homo sapiens. [Contig view]
GeneID 1392; -.
KEGG hsa:1392; -.
Phylogenomic databases
HOGENOM P06850; -.
HOVERGEN P06850; -.
OMA P06850; AANFFRV.
Other
DrugBank DB01285; Corticotropin.
NextBio 5695; -.
SOURCE CRH; Homo sapiens.
ProtoNet P06850.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Amidation; Cleavage on pair of basic residues; Direct protein sequencing; Hormone; Secreted; Signal.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    24  24     Probable. 
PROPEP   25   153  129      PRO_0000006212
PEPTIDE   154   194  41     Corticoliberin. PRO_0000006213
MOD_RES   194   194        Isoleucine amide. 
Sequence information
Length: 196 AA [This is the length of the unprocessed precursor] Molecular weight: 21422 Da [This is the MW of the unprocessed precursor] CRC64: 0CCDFF05BE364E92 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MRLPLLVSAG VLLVALLPCP PCRALLSRGP VPGARQAPQH PQPLDFFQPP PQSEQPQQPQ 

        70         80         90        100        110        120 
ARPVLLRMGE EYFLRLGNLN KSPAAPLSPA SSLLAGGSGS RPSPEQATAN FFRVLLQQLL 

       130        140        150        160        170        180 
LPRRSLDSPA ALAERGARNA LGGHQEAPER ERRSEEPPIS LDLTFHLLRE VLEMARAEQL 

       190 
AQQAHSNRKL MEIIGK 

P06850 in FASTA format

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BLAST logo BLAST submission on ExPASy/SIB
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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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NPSA logo NPSA Sequence analysis tools

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