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UniProtKB/Swiss-Prot entry P06832

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NPRE_BACAM
Primary accession number P06832
Secondary accession numbers None
Integrated into Swiss-Prot on January 1, 1988
Sequence was last modified on January 1, 1988 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 69)
Name and origin of the protein
Protein name Bacillolysin [Precursor]
Synonyms EC 3.4.24.28
Neutral protease
Gene name
Name: npr
From
Bacillus amyloliquefaciens [TaxID: 1390] 
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 23844 / P;
PubMed=6090391 [NCBI, ExPASy, EBI, Israel, Japan]
Vasantha N., Thompson L.D., Rhodes C., Banner C., Nagle J., Filpula D.;
"Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein.";
J. Bacteriol. 159:811-819(1984).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
K02497; AAB05346.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A25415; HYBSN.
3D structure databases
HSSP P81177; 1BQB. [HSSP ENTRY / PDB]
ModBase P06832.
Protein family/group databases
MEROPS M04.014; -.
Ontologies
GO
GO:0005509; Molecular function: calcium ion binding (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR005075; Pept_M4_propep_PepSY.
IPR006025; Pept_M_Zn_BS.
IPR013856; Peptidase_M4.
IPR001570; Peptidase_M4_C.
IPR011096; Propep_M4_M36.
Graphical view of domain structure.
Gene3D G3DSA:3.10.170.10; Peptidase_M4; 1.
G3DSA:1.10.390.10; Peptidase_M4/M36; 1.
Pfam PF07504; FTP; 1.
PF03413; PepSY; 1.
PF01447; Peptidase_M4; 1.
PF02868; Peptidase_M4_C; 1.
Pfam graphical view of domain structure.
PRINTS PR00730; THERMOLYSIN.
PROSITE PS00142; ZINC_PROTEASE; 1.
BLOCKS P06832.
ProtoNet P06832.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Calcium; Hydrolase; Metal-binding; Metalloprotease; Protease; Secreted; Signal; Zinc; Zymogen.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    27  27     Potential. 
PROPEP   28   221  194     Activation peptide. PRO_0000028594
CHAIN   222   521  300     Bacillolysin. PRO_0000028595
ACT_SITE   365   365        By similarity. 
ACT_SITE   449   449        Proton donor (By similarity). 
METAL   283   283        Calcium 1; via carbonyl oxygen (Potential). 
METAL   360   360        Calcium 2 (Potential). 
METAL   364   364        Zinc; catalytic (By similarity). 
METAL   368   368        Zinc; catalytic (By similarity). 
METAL   388   388        Zinc; catalytic (By similarity). 
METAL   399   399        Calcium 2 (Potential). 
METAL   399   399        Calcium 3 (Potential). 
METAL   402   402        Calcium 2 (Potential). 
METAL   402   402        Calcium 3 (Potential). 
METAL   404   404        Calcium 2; via carbonyl oxygen (Potential). 
METAL   407   407        Calcium 2 (Potential). 
METAL   407   407        Calcium 3 (Potential). 
METAL   411   411        Calcium 4; via carbonyl oxygen (Potential). 
Sequence information
Length: 521 AA [This is the length of the unprocessed precursor] Molecular weight: 56840 Da [This is the MW of the unprocessed precursor] CRC64: 9609FA0416222751 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MGLGKKLSVA VAASFMSLTI SLPGVQAAEN PQLKENLTNF VPKHSLVQSE LPSVSDKAIK 

        70         80         90        100        110        120 
QYLKQNGKVF KGNPSERLKL IDQTTDDLGY KHFRYVPVVN GVPVKDSQVI IHVDKSNNVY 

       130        140        150        160        170        180 
AINGELNNDV SAKTANSKKL SANQALDHAY KAIGKSPEAV SNGTVANKNK AELKAAATKD 

       190        200        210        220        230        240 
GKYRLAYDVT IRYIEPEPAN WEVTVDAETG KILKKQNKVE HAATTGTGTT LKGKTVSLNI 

       250        260        270        280        290        300 
SSESGKYVLR DLSKPTGTQI ITYDLQNREY NLPGTLVSST TNQFTTSSQR AAVDAHYNLG 

       310        320        330        340        350        360 
KVYDYFYQKF NRNSYDNKGG KIVSSVHYGS RYNNAAWIGD QMIYGDGDGS FFSPLSGSMD 

       370        380        390        400        410        420 
VTAHEMTHGV TQETANLNYE NQPGALNESF SDVFGYFNDT EDWDIGEDIT VSQPALRSLS 

       430        440        450        460        470        480 
NPTKYGQPDN FKNYKNLPNT DAGDYGGVHT NSGIPNKAAY NTITKIGVNK AEQIYYRALT 

       490        500        510        520 
VYLTPSSTFK DAKAALIQSA RDLYGSQDAA SVEAAWNAVG L
P06832 in FASTA format

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