ID   MAOX_MOUSE              Reviewed;         572 AA.
AC   P06801;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   22-JUL-2008, entry version 75.
DE   RecName: Full=NADP-dependent malic enzyme;
DE            Short=NADP-ME;
DE            EC=1.1.1.40;
DE   AltName: Full=Malic enzyme 1;
GN   Name=Me1; Synonyms=Mod-1, Mod1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=87109297; PubMed=3805042;
RA   Bagchi S., Wise L.S., Brown M.L., Bregman D., Sul H.S., Rubin C.S.;
RT   "Structure and expression of murine malic enzyme mRNA.
RT   Differentiation-dependent accumulation of two forms of malic enzyme
RT   mRNA in 3T3-L1 cells.";
RL   J. Biol. Chem. 262:1558-1565(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Liver;
RX   MEDLINE=87098422; PubMed=3541755;
RA   Bagchi S., Wise L.S., Brown M.L., Sul H.S., Bregman D.B., Rubin C.S.;
RT   "Regulation and structure of murine malic enzyme mRNA.";
RL   Ann. N. Y. Acad. Sci. 478:77-92(1986).
RN   [3]
RP   PROTEIN SEQUENCE OF 428-445, AND MASS SPECTROMETRY.
RC   TISSUE=Hippocampus;
RA   Lubec G., Klug S.;
RL   Submitted (MAR-2007) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NADP(+) = pyruvate + CO(2) +
CC       NADPH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
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DR   EMBL; J02652; AAA39727.1; -; mRNA.
DR   EMBL; M26756; AAA39489.1; -; mRNA.
DR   PIR; A26683; DEMSMX.
DR   UniGene; Mm.148155; -.
DR   HSSP; P40927; 1GQ2.
DR   SWISS-2DPAGE; P06801; -.
DR   REPRODUCTION-2DPAGE; P06801; -.
DR   Ensembl; ENSMUSG00000032418; Mus musculus.
DR   MGI; MGI:97043; Me1.
DR   HOGENOM; P06801; -.
DR   HOVERGEN; P06801; -.
DR   ArrayExpress; P06801; -.
DR   CleanEx; MM_ME1; -.
DR   GermOnline; ENSMUSG00000032418; Mus musculus.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0005625; C:soluble fraction; IDA:MGI.
DR   GO; GO:0004473; F:malate dehydrogenase (oxaloacetate-decarbox...; ISS:UniProtKB.
DR   GO; GO:0030145; F:manganese ion binding; ISS:UniProtKB.
DR   GO; GO:0006108; P:malate metabolic process; ISS:UniProtKB.
DR   GO; GO:0009725; P:response to hormone stimulus; ISS:UniProtKB.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Metal-binding; NADP;
KW   Oxidoreductase.
FT   CHAIN         1    572       NADP-dependent malic enzyme.
FT                                /FTId=PRO_0000160193.
FT   NP_BIND     301    318       NADP (By similarity).
FT   ACT_SITE    102    102       Proton donor (By similarity).
FT   ACT_SITE    173    173       Proton acceptor (By similarity).
FT   METAL       245    245       Divalent metal cation (By similarity).
FT   METAL       246    246       Divalent metal cation (By similarity).
FT   METAL       269    269       Divalent metal cation (By similarity).
FT   BINDING     155    155       NADP (By similarity).
FT   BINDING     269    269       NADP (By similarity).
FT   SITE        269    269       Important for activity (By similarity).
SQ   SEQUENCE   572 AA;  63999 MW;  86B1761D04C2B51E CRC64;
     MEPRAPRRRH THQRGYLLTR DPHLNKDLAF TLEERQQLNI HGLLPPCIIS QELQVLRIIK
     NFERLNSDFD RYLLLMDLQD RNEKLFYSVL MSDVEKFMPI VYTPTVGLAC QQYSLAFRKP
     RGLFISIHDK GHIASVLNAW PEDVVKAIVV TDGERILGLG DLGCNGMGIP VGKLALYTAC
     GGVNPQQCLP ITLDVGTENE ELLKDPLYIG LRHRRVRGPE YDAFLDEFME AASSKYGMNC
     LIQFEDFANR NAFRLLNKYR NKYCTFNDDI QGTASVAVAG LLAALRITKN KLSDQTVLFQ
     GAGEAALGIA HLVVMAMEKE GLSKENARKK IWLVDSKGLI VKGRASLTEE KEVFAHEHEE
     MKNLEAIVQK IKPTALIGVA AIGGAFTEQI LKDMAAFNER PIIFALSSPT SKAECSADEC
     YKVTKGRAIF ASGSPFDPVT LPDGRTLFPG QGNNSYVFPG VALGVVACGL RHIDDKVFLT
     TREVISQQVS DKHLQEGRLY PPLNTIRGVS LKIAVKIVQD AYKEKMATVY PEPQNKEEFV
     SSQMYSTNYD QILPDCYPWP AEVQKIQTKV NQ
//