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UniProtKB/Swiss-Prot entry P06738


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PHSG_YEAST
Primary accession number P06738
Secondary accession numbers None
Integrated into Swiss-Prot on January 1, 1988
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    September 2, 2008 (Entry version 94)
Name and origin of the protein
Protein name Glycogen phosphorylase
Synonym EC 2.4.1.1
Gene name
Name: GPH1
OrderedLocusNames: YPR160W
ORFNames: P9584.1
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1038/324080a0; PubMed=3537803 [NCBI, ExPASy, EBI, Israel, Japan]
Hwang P.K., Fletterick R.J.;
"Convergent and divergent evolution of regulatory sites in eukaryotic phosphorylases.";
Nature 324:80-84(1986).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1002/yea.320101117; PubMed=7871892 [NCBI, ExPASy, EBI, Israel, Japan]
Roemer T.D., Fortin N., Bussey H.;
"DNA sequence analysis of a 10.4 kbp region on the right arm of yeast chromosome XVI positions GPH1 and SGV1 adjacent to KRE6, and identifies two novel tRNA genes.";
Yeast 10:1527-1530(1994).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204511 / S288c / AB972;
PubMed=9169875 [NCBI, ExPASy, EBI, Israel, Japan]
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
Nature 387:103-105(1997).
[4]
PROTEIN SEQUENCE OF 2-10, AND PHOSPHORYLATION AT THR-31.
DOI=10.1016/0022-2836(92)90102-P; PubMed=1613787 [NCBI, ExPASy, EBI, Israel, Japan]
Rath V.L., Hwang P.K., Fletterick R.J.;
"Purification and crystallization of glycogen phosphorylase from Saccharomyces cerevisiae.";
J. Mol. Biol. 225:1027-1034(1992).
[5]
PROTEIN SEQUENCE OF 30-37 AND 737-754, PHOSPHORYLATION AT THR-31, AND PYRIDOXAL PHOSPHATE AT LYS-751.
DOI=10.1021/bi00680a031; PubMed=1092346 [NCBI, ExPASy, EBI, Israel, Japan]
Lerch K., Fischer E.H.;
"Amino acid sequence of two functional sites in yeast glycogen phosphorylase.";
Biochemistry 14:2009-2014(1975).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-31, AND MASS SPECTROMETRY.
DOI=10.1021/pr060559j; PubMed=17330950 [NCBI, ExPASy, EBI, Israel, Japan]
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.;
"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.";
J. Proteome Res. 6:1190-1197(2007).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-31, AND MASS SPECTROMETRY.
DOI=10.1073/pnas.0701622104; PubMed=17563356 [NCBI, ExPASy, EBI, Israel, Japan]
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.;
"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases.";
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007).
[8]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-18; SER-19; THR-31 AND SER-333, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
[9]
X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 14-901.
PubMed=8703213 [NCBI, ExPASy, EBI, Israel, Japan]
Lin K., Rath V.L., Dai S.C., Fletterick R.J., Hwang P.K.;
"A protein phosphorylation switch at the conserved allosteric site in GP.";
Science 273:1539-1542(1996).
[10]
SUBUNIT.
DOI=10.1016/0003-9861(83)90078-4; PubMed=6354094 [NCBI, ExPASy, EBI, Israel, Japan]
Becker J.U., Wingender-Drissen R., Schiltz E.;
"Purification and properties of phosphorylase from baker's yeast.";
Arch. Biochem. Biophys. 225:667-678(1983).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X04604; CAA28273.1; ALT_INIT; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
L33835; AAB59313.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U28371; AAB68057.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S61144; S61144.
3D structure databases
PDB
1YGP; X-ray; 2.80 A; A/B=24-902.[ExPASy / RCSB / EBI]
PDBsum 1YGP; -.
ModBase P06738.
Protein-protein interaction databases
DIP DIP:2648N; -.
IntAct P06738; -.
Enzyme and pathway databases
BioCyc MetaCyc:MON-13684; -.
Organism-specific databases
CYGD YPR160w; -.
SGD S000006364; GPH1.
Yeast-GFP YPR160W.
Gene expression databases
GermOnline YPR160W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from direct assay from SGD).
GO:0008184; Molecular function: glycogen phosphorylase activity (inferred from mutant phenotype from SGD).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0005980; Biological process: glycogen catabolic process (inferred from mutant phenotype from SGD).
QuickGo view.
Family and domain databases
InterPro IPR011833; Glycg_phsphrylas.
IPR000811; Glyco_trans_35.
Graphical view of domain structure.
PANTHER PTHR11468; Glyco_trans_35; 1.
Pfam PF00343; Phosphorylase; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000460; Pprylas_GlgP; 1.
TIGRFAMs TIGR02093; P_ylase; 1.
PROSITE PS00102; PHOSPHORYLASE; 1.
BLOCKS P06738.
Proteomic databases
PeptideAtlas P06738; -.
Genome annotation databases
Ensembl YPR160W; Saccharomyces cerevisiae. [Contig view]
GenomeReviews U00094_GR; YPR160W.
Phylogenomic databases
HOGENOM P06738; -.
Other
LinkHub P06738; -.
ProtoNet P06738.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Carbohydrate metabolism; Complete proteome; Direct protein sequencing; Glycogen metabolism; Glycosyltransferase; Phosphoprotein; Pyridoxal phosphate; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   902  901     Glycogen phosphorylase. PRO_0000188545
BINDING   751   751        Pyridoxal phosphate (covalent). 
MOD_RES   18    18        Phosphothreonine. 
MOD_RES   19    19        Phosphoserine. 
MOD_RES   31    31        Phosphothreonine. 
MOD_RES   333   333        Phosphoserine. 
CONFLICT   167   168        EG -> DL (in Ref. 3; AAB68057). 
CONFLICT   326   326        P -> A (in Ref. 3; AAB68057). 
CONFLICT   440   441        RR -> VG (in Ref. 3; AAB68057). 
CONFLICT   508   508        V -> A (in Ref. 3; AAB68057). 
CONFLICT   524   524        I -> V (in Ref. 3; AAB68057). 
CONFLICT   578   578        E -> G (in Ref. 3; AAB68057). 
CONFLICT   876   876        L -> V (in Ref. 3; AAB68057). 
HELIX   63    75  13      
HELIX   82    84  3      
HELIX   87   116  30      
STRAND   120   124  5      
HELIX   133   139  7      
HELIX   158   161  4      
HELIX   164   167  4      
HELIX   171   175  5      
HELIX   187   201  15      
STRAND   206   211  6      
STRAND   219   223  5      
STRAND   226   230  5      
TURN   234   237  4      
STRAND   243   255  13      
STRAND   257   259  3      
STRAND   272   274  3      
STRAND   276   289  14      
STRAND   296   307  12      
HELIX   312   316  5      
HELIX   320   323  4      
HELIX   325   335  11      
STRAND   336   338  3      
HELIX   344   371  28      
HELIX   376   378  3      
HELIX   379   382  4      
STRAND   383   390  8      
TURN   391   393  3      
HELIX   394   405  12      
HELIX   411   421  11      
STRAND   422   426  5      
HELIX   431   433  3      
STRAND   436   438  3      
HELIX   439   445  7      
HELIX   447   467  21      
HELIX   473   478  6      
STRAND   480   482  3      
STRAND   485   487  3      
STRAND   489   491  3      
HELIX   492   499  8      
STRAND   500   507  8      
HELIX   508   516  9      
TURN   517   519  3      
HELIX   520   526  7      
HELIX   528   530  3      
STRAND   531   533  3      
HELIX   540   543  4      
TURN   544   547  4      
HELIX   549   558  10      
HELIX   565   567  3      
HELIX   570   581  12      
HELIX   584   606  23      
TURN   607   610  4      
HELIX   619   621  3      
STRAND   623   629  7      
HELIX   633   635  3      
HELIX   637   656  20      
HELIX   661   667  7      
STRAND   671   676  6      
HELIX   684   700  17      
HELIX   704   706  3      
STRAND   709   715  7      
HELIX   720   726  7      
HELIX   727   729  3      
STRAND   731   735  5      
HELIX   746   752  7      
TURN   753   755  3      
STRAND   757   762  6      
HELIX   765   773  9      
HELIX   775   777  3      
STRAND   778   782  5      
HELIX   785   797  13      
HELIX   804   812  9      
TURN   823   826  4      
HELIX   827   835  9      
TURN   837   840  4      
HELIX   842   862  21      
HELIX   864   878  15      
HELIX   879   881  3      
HELIX   883   893  11      
Sequence information
Length: 902 AA [This is the length of the unprocessed precursor] Molecular weight: 103544 Da [This is the MW of the unprocessed precursor] CRC64: 049C5A6F4E4A4826 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MPPASTSTTN DMITEEPTSP HQIPRLTRRL TGFLPQEIKS IDTMIPLKSR ALWNKHQVKK 

        70         80         90        100        110        120 
FNKAEDFQDR FIDHVETTLA RSLYNCDDMA AYEAASMSIR DNLVIDWNKT QQKFTTRDPK 

       130        140        150        160        170        180 
RVYYLSLEFL MGRALDNALI NMKIEDPEDP AASKGKPREM IKGALDEGGF KLEDVLDQEP 

       190        200        210        220        230        240 
DAGLGNGGLG RLAACFVDSM ATEGIPAWGY GLRYEYGIFA QKIIDGYQVE TPDYWLNSGN 

       250        260        270        280        290        300 
PWEIERNEVQ IPVTFYGYVD RPEGGKTTLS ASQWIGGERV LAVAYDFPVP GFKTSNVNNL 

       310        320        330        340        350        360 
RLWQARPTTE FDFAKFNNGD YKNSVPQQQR AESITAVLYP NDNFAQGKEL RLKQQYFWCA 

       370        380        390        400        410        420 
ASLHDILRRF KKSKRPWTEF PDQVAIQLND THPTLAIVEL QRVLVDLEKL DWHEAWDIVT 

       430        440        450        460        470        480 
KTFAYTNHTV MQEALEKWPR RLFGHLLPRH LEIIYDINWF FLQDVAKKFP KDVDLLSRIS 

       490        500        510        520        530        540 
IIEENSPERQ IRMAFLAIVG SHKVNGVVEL HSELIKTTIF KDFIKFYGPS KFVNVTNGIT 

       550        560        570        580        590        600 
PRRWLKQANP SLAKLISETL NDPTEEYLLD MAKLTQLEKY VEDKEFLKKW NQVKLNNKIR 

       610        620        630        640        650        660 
LVDLIKKEND GVDIINREYL DDTLFDMQVK RIHEYKRQQL NVFGIIYRYL AMKNMLKNGA 

       670        680        690        700        710        720 
SIEEVAKKYP RKVSIFGGKS APGYYMAKLI IKLINCVADI VNNDESIEHL LKVVFVADYN 

       730        740        750        760        770        780 
VSKAEIIIPA SDLSEHISTA GTEASGTSNM KFVMNGGLII GTVDGANVEI TREIGEDNVF 

       790        800        810        820        830        840 
LFGNLSENVE ELRYNHQYHP QDLPSSLDSV LSYIESGQFS PENPNEFKPL VDSIKYHGDY 

       850        860        870        880        890        900 
YLVSDDFESY LATHELVDQE FHNQRSEWLK KSVLSLANVG FFSSDRCIEE YSDTIWNVEP 


VT 

P06738 in FASTA format

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