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UniProtKB/Swiss-Prot entry P06715


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GSHR_ECOLI
Primary accession number P06715
Secondary accession number Q2M7G2
Integrated into Swiss-Prot on January 1, 1988
Sequence was last modified on January 1, 1988 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 103)
Name and origin of the protein
Protein name Glutathione reductase
Synonyms GRase
GR
EC 1.8.1.7
Gene name
Name: gor
OrderedLocusNames: b3500, JW3467
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=K12;
DOI=10.1021/bi00357a069; PubMed=3521741 [NCBI, ExPASy, EBI, Israel, Japan]
Greer S., Perham R.N.;
"Glutathione reductase from Escherichia coli: cloning and sequence analysis of the gene and relationship to other flavoprotein disulfide oxidoreductases.";
Biochemistry 25:2736-2742(1986).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1093/nar/22.13.2576; PubMed=8041620 [NCBI, ExPASy, EBI, Israel, Japan]
Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes.";
Nucleic Acids Res. 22:2576-2586(1994).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[5]
X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
DOI=10.1002/prot.340090303; PubMed=2006135 [NCBI, ExPASy, EBI, Israel, Japan]
Ermler U., Schulz G.E.;
"The three-dimensional structure of glutathione reductase from Escherichia coli at 3.0-A resolution.";
Proteins 9:174-179(1991).
[6]
X-RAY CRYSTALLOGRAPHY (1.86 ANGSTROMS), AND DISULFIDE BOND.
PubMed=8061609 [NCBI, ExPASy, EBI, Israel, Japan]
Mittl P.R.E., Schulz G.E.;
"Structure of glutathione reductase from Escherichia coli at 1.86-A resolution: comparison with the enzyme from human erythrocytes.";
Protein Sci. 3:799-809(1994).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M13141; AAA23926.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00039; AAB18476.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC76525.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAE77794.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A24409; RDECU.
RefSeq AP_004293.1; -.
NP_417957.1; -.
3D structure databases
PDB
1GER; X-ray; 1.86 A; A/B=1-450.[ExPASy / RCSB / EBI]
1GES; X-ray; 1.74 A; A/B=1-450.[ExPASy / RCSB / EBI]
1GET; X-ray; 2.00 A; A/B=1-450.[ExPASy / RCSB / EBI]
1GEU; X-ray; 2.20 A; A/B=1-450.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1GER; -.
1GES; -.
1GET; -.
1GEU; -.
ModBase P06715.
Protein-protein interaction databases
IntAct P06715; -.
Enzyme and pathway databases
BioCyc EcoCyc:GLUTATHIONE-REDUCT-NADPH-MON; -.
MetaCyc:GLUTATHIONE-REDUCT-NADPH-MON; -.
2D gel databases
SWISS-2DPAGE P06715; -.
ECO2DBASE F045.6; 6TH EDITION.
Organism-specific databases
EchoBASE EB0407; -.
EcoGene EG10412; gor.
Ontologies
GO
GO:0005739; Cellular component: mitochondrion (inferred from direct assay from UniProtKB).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR013027; FAD_pyr_nucl-diS_OxRdtase.
IPR006322; Glut_reduct_1.
IPR000815; Hg_reductase.
IPR001100; Pyr_nuc-diS_OxRdtase.
IPR004099; Pyr_nucl-diS_OxRdtase_dimer.
IPR012999; Pyr_OxRdtase_I_AS.
IPR001327; Pyr_OxRdtase_NAD_bd.
Graphical view of domain structure.
Gene3D G3DSA:3.30.390.30; Pyr_redox_dim; 1.
Pfam PF00070; Pyr_redox; 1.
PF07992; Pyr_redox_2; 1.
PF02852; Pyr_redox_dim; 1.
Pfam graphical view of domain structure.
PRINTS PR00368; FADPNR.
PR00945; HGRDTASE.
PR00411; PNDRDTASEI.
ProDom PD000139; FAD_pyr_redox; 1.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR01421; gluta_reduc_1; 1.
PROSITE PS00076; PYRIDINE_REDOX_1; 1.
BLOCKS P06715.
Other
SWISS-3DIMAGE P06715.
Genome annotation databases
GeneID 948014; -.
GenomeReviews U00096_GR; b3500.
AP009048_GR; JW3467.
KEGG ecj:JW3467; -.
eco:b3500; -.
Phylogenomic databases
HOGENOM P06715; -.
Other
DrugBank DB00336; Nitrofurazone.
LinkHub P06715; -.
Genome annotation databases
CMR P06715; b3500.
Other
ProtoNet P06715.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Complete proteome; Cytoplasm; FAD; Flavoprotein; NADP; Oxidoreductase; Redox-active center.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   450  450     Glutathione reductase. PRO_0000067975
NP_BIND   34    41  8     FAD. 
ACT_SITE   439   439        Proton acceptor. 
DISULFID   42    47        Redox-active. 
STRAND   4    10  7      
HELIX   14    25  12      
STRAND   30    36  7      
HELIX   40    45  6      
HELIX   47    64  18      
HELIX   67    70  4      
STRAND   72    79  8      
HELIX   81   105  25      
STRAND   109   113  5      
STRAND   116   119  4      
STRAND   122   125  4      
STRAND   128   137  10      
STRAND   141   143  3      
HELIX   151   153  3      
HELIX   157   161  5      
STRAND   168   173  6      
HELIX   177   188  12      
STRAND   192   196  5      
STRAND   198   202  5      
HELIX   208   221  14      
STRAND   224   226  3      
STRAND   231   236  6      
STRAND   242   246  5      
STRAND   251   259  9      
STRAND   263   265  3      
HELIX   272   275  4      
STRAND   298   300  3      
HELIX   303   305  3      
HELIX   311   326  16      
STRAND   341   343  3      
STRAND   349   353  5      
HELIX   356   363  8      
TURN   365   367  3      
STRAND   368   376  9      
HELIX   378   381  4      
STRAND   383   385  3      
STRAND   388   396  9      
TURN   397   400  4      
STRAND   401   409  9      
HELIX   412   425  14      
HELIX   429   433  5      
HELIX   443   447  5      
Sequence information
Length: 450 AA [This is the length of the unprocessed precursor] Molecular weight: 48773 Da [This is the MW of the unprocessed precursor] CRC64: 3C8652AFE4E4ABF6 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MTKHYDYIAI GGGSGGIASI NRAAMYGQKC ALIEAKELGG TCVNVGCVPK KVMWHAAQIR 

        70         80         90        100        110        120 
EAIHMYGPDY GFDTTINKFN WETLIASRTA YIDRIHTSYE NVLGKNNVDV IKGFARFVDA 

       130        140        150        160        170        180 
KTLEVNGETI TADHILIATG GRPSHPDIPG VEYGIDSDGF FALPALPERV AVVGAGYIAV 

       190        200        210        220        230        240 
ELAGVINGLG AKTHLFVRKH APLRSFDPMI SETLVEVMNA EGPQLHTNAI PKAVVKNTDG 

       250        260        270        280        290        300 
SLTLELEDGR SETVDCLIWA IGREPANDNI NLEAAGVKTN EKGYIVVDKY QNTNIEGIYA 

       310        320        330        340        350        360 
VGDNTGAVEL TPVAVAAGRR LSERLFNNKP DEHLDYSNIP TVVFSHPPIG TVGLTEPQAR 

       370        380        390        400        410        420 
EQYGDDQVKV YKSSFTAMYT AVTTHRQPCR MKLVCVGSEE KIVGIHGIGF GMDEMLQGFA 

       430        440        450 
VALKMGATKK DFDNTVAIHP TAAEEFVTMR 

P06715 in FASTA format

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View entry in raw text format (no links)
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