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UniProtKB/Swiss-Prot entry P06702


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name S10A9_HUMAN
Primary accession number P06702
Secondary accession numbers Q6FGA1 Q9NYM0 Q9UCJ1
Integrated into Swiss-Prot on January 1, 1988
Sequence was last modified on January 1, 1988 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 107)
Name and origin of the protein
Protein name Protein S100-A9
Synonyms S100 calcium-binding protein A9
Calgranulin-B
Migration inhibitory factor-related protein 14
MRP-14
P14
Leukocyte L1 complex heavy chain
Calprotectin L1H subunit
Gene name
Name: S100A9
Synonyms: CAGB, CFAG, MRP14
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1038/330080a0; PubMed=3313057 [NCBI, ExPASy, EBI, Israel, Japan]
Odink K., Cerletti N., Bruggen J., Clerc R.G., Tarcsay L., Zwaldo G., Gerhards G., Schlegel R., Sorg C.;
"Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis.";
Nature 330:80-82(1987).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=3405210 [NCBI, ExPASy, EBI, Israel, Japan]
Lagasse E., Clerc R.G.;
"Cloning and expression of two human genes encoding calcium-binding proteins that are regulated during myeloid differentiation.";
Mol. Cell. Biol. 8:2402-2410(1988).
[3]
NUCLEOTIDE SEQUENCE [MRNA], AND BLOCKED N-TERMINUS.
PubMed=2656677 [NCBI, ExPASy, EBI, Israel, Japan]
Murao S., Collart F.R., Huberman E.;
"A protein containing the cystic fibrosis antigen is an inhibitor of protein kinases.";
J. Biol. Chem. 264:8356-8360(1989).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT ARG-20.
Wang M., Xu X., Cai Y., Xu H., Han Y., Xu Z., Wu M.;
"Human gene for migration inhibitory factor-related protein 14 (MRP14), variant allele.";
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.;
"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201).";
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
[6]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature04727; PubMed=16710414 [NCBI, ExPASy, EBI, Israel, Japan]
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
"The DNA sequence and biological annotation of human chromosome 1.";
Nature 441:315-321(2006).
[7]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Lung;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[8]
PROTEIN SEQUENCE OF 84-114, AND PHOSPHORYLATION AT THR-113.
DOI=10.1038/342189a0; PubMed=2478889 [NCBI, ExPASy, EBI, Israel, Japan]
Edgeworth J., Freemont P., Hogg N.;
"Ionomycin-regulated phosphorylation of the myeloid calcium-binding protein p14.";
Nature 342:189-192(1989).
[9]
PROTEIN SEQUENCE, AND BLOCKED N-TERMINUS.
PubMed=2776242 [NCBI, ExPASy, EBI, Israel, Japan]
Tobe T., Murakami K., Tomita M., Nozawa R.;
"Amino acid sequences of 60B8 antigens induced in HL-60 cells by 1,25-dihydroxyvitamin D3. The antigens are identical with macrophage-related protein-14 and -8.";
Chem. Pharm. Bull. 37:1576-1580(1989).
[10]
PROTEIN SEQUENCE OF 11-19; 26-37 AND 94-107.
TISSUE=Keratinocyte;
DOI=10.1002/elps.11501301199; PubMed=1286667 [NCBI, ExPASy, EBI, Israel, Japan]
Rasmussen H.H., van Damme J., Puype M., Gesser B., Celis J.E., Vandekerckhove J.;
"Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes.";
Electrophoresis 13:960-969(1992).
[11]
PROTEIN SEQUENCE OF 5-34.
PubMed=8423249 [NCBI, ExPASy, EBI, Israel, Japan]
Miyasaki K.T., Bodeau A.L., Murthy A.R., Lehrer R.I.;
"In vitro antimicrobial activity of the human neutrophil cytosolic S-100 protein complex, calprotectin, against Capnocytophaga sputigena.";
J. Dent. Res. 72:517-523(1993).
[12]
INTERACTION WITH CEACAM3.
DOI=10.1006/bbrc.2001.5955; PubMed=11708798 [NCBI, ExPASy, EBI, Israel, Japan]
Streichert T., Ebrahimnejad A., Ganzer S., Flayeh R., Wagener C., Bruemmer J.;
"The microbial receptor CEACAM3 is linked to the calprotectin complex in granulocytes.";
Biochem. Biophys. Res. Commun. 289:191-197(2001).
[13]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-113, AND MASS SPECTROMETRY.
TISSUE=Liver;
DOI=10.1002/pmic.200700884; PubMed=18318008 [NCBI, ExPASy, EBI, Israel, Japan]
Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J.;
"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography.";
Proteomics 8:1346-1361(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X06233; CAA29579.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M21064; AAA36326.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M26311; AAA68480.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF237581; AAF62536.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF237582; AAF62537.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CR542207; CAG47003.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CR542224; CAG47020.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL591704; CAI19494.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC047681; AAH47681.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR B31848; B31848.
RefSeq NP_002956.1; -.
UniGene Hs.112405
3D structure databases
PDB
1IRJ; X-ray; 2.10 A; A/B/C/D/E/F/G/H=2-114.[ExPASy / RCSB / EBI]
1XK4; X-ray; 1.80 A; C/D/G/H/K/L=2-114.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1IRJ; -.
1XK4; -.
ModBase P06702.
Protein-protein interaction databases
DIP DIP:1166N; -.
IntAct P06702; -.
PTM databases
PhosphoSite P06702; -.
2D gel databases
SWISS-2DPAGE P06702; -.
Aarhus/Ghent-2DPAGE 5007; IEF.
6010; IEF.
6017; IEF.
7013; IEF.
OGP P06702; -.
PMMA-2DPAGE P06702; -.
Organism-specific databases
H-InvDB HIX0023536; -.
HGNC HGNC:10499; S100A9.
GenAtlas S100A9.
HPA CAB009441; -.
HPA004193; -.
MIM 123886; gene. [NCBI / EBI]
PharmGKB PA34911; -.
GeneCards P06702.
Gene expression databases
ArrayExpress P06702; -.
CleanEx HS_S100A9; -.
GermOnline ENSG00000163220; Homo sapiens.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from direct assay from HPA).
GO:0005634; Cellular component: nucleus (inferred from direct assay from HPA).
GO:0005509; Molecular function: calcium ion binding (traceable author statement from ProtInc).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0004871; Molecular function: signal transducer activity (traceable author statement from ProtInc).
GO:0007267; Biological process: cell-cell signaling (traceable author statement from ProtInc).
GO:0006954; Biological process: inflammatory response (traceable author statement from ProtInc).
QuickGo view.
Family and domain databases
InterPro IPR011992; EF-Hand_type.
IPR002048; EF_hand_Ca_bd.
IPR001751; S100_Ca_bd.
IPR013787; S100_Ca_bd_sub.
Graphical view of domain structure.
Gene3D G3DSA:1.10.238.10; EF-Hand_type; 1.
Pfam PF00036; efhand; 1.
PF01023; S_100; 1.
Pfam graphical view of domain structure.
ProDom PD003407; CaBP_S100; 1.
PD000012; EF-hand; 1.
[Domain structure / List of seq. sharing at least 1 domain]
PROSITE PS00018; EF_HAND_1; 1.
PS50222; EF_HAND_2; 1.
PS00303; S100_CABP; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P06702.
ProtoNet P06702.
Proteomic databases
PeptideAtlas P06702; -.
Genome annotation databases
Ensembl ENSG00000163220; Homo sapiens. [Contig view]
GeneID 6280; -.
KEGG hsa:6280; -.
Phylogenomic databases
HOGENOM P06702; -.
HOVERGEN P06702; -.
Other
NextBio 24377; -.
SOURCE S100A9; Homo sapiens.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Calcium; Direct protein sequencing; Phosphoprotein; Polymorphism; Repeat.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   114  114     Protein S100-A9. PRO_0000143997
DOMAIN   12    47  36     EF-hand 1. 
DOMAIN   54    89  36     EF-hand 2. 
CA_BIND   23    36  14     1; low affinity (Potential). 
CA_BIND   67    78  12     2; high affinity (Potential). 
MOD_RES   2     2        Blocked amino end (Thr). 
MOD_RES   113   113        Phosphothreonine. 
VARIANT   20    20  1     H -> R. VAR_013008 [3D]
CONFLICT   6     6        S -> H (in Ref. 11; AA sequence). 
CONFLICT   25    25        K -> F (in Ref. 11; AA sequence). 
CONFLICT   28    28        H -> L (in Ref. 11; AA sequence). 
HELIX   7    23  17      
STRAND   25    28  4      
HELIX   34    44  11      
TURN   46    51  6      
HELIX   52    54  3      
HELIX   56    66  11      
HELIX   76    79  4      
HELIX   81    85  5      
HELIX   87    91  5      
Sequence information
Length: 114 AA [This is the length of the unprocessed precursor] Molecular weight: 13242 Da [This is the MW of the unprocessed precursor] CRC64: C3BE19729E14C078 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MTCKMSQLER NIETIINTFH QYSVKLGHPD TLNQGEFKEL VRKDLQNFLK KENKNEKVIE 

        70         80         90        100        110 
HIMEDLDTNA DKQLSFEEFI MLMARLTWAS HEKMHEGDEG PGHHHKPGLG EGTP 

P06702 in FASTA format

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