ID   PA21B_CANFA             Reviewed;         146 AA.
AC   P06596;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   04-NOV-2008, entry version 74.
DE   RecName: Full=Phospholipase A2;
DE            EC=3.1.1.4;
DE   AltName: Full=Phosphatidylcholine 2-acylhydrolase;
DE   AltName: Full=Group IB phospholipase A2;
DE   Flags: Precursor;
GN   Name=PLA2G1B;
OS   Canis familiaris (Dog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae;
OC   Canis.
OX   NCBI_TaxID=9615;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=86223862; PubMed=3754861;
RA   Ohara O., Tamaki M., Nakamura E., Tsuruta Y., Fujii Y., Shin M.,
RA   Teraoka H., Okamoto M.;
RT   "Dog and rat pancreatic phospholipases A2: complete amino acid
RT   sequences deduced from complementary DNAs.";
RL   J. Biochem. 99:733-739(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=90306027; PubMed=2142076;
RA   Kerfelec B., Laforge K.S., Vasiloudes P., Puigserver A., Scheele G.A.;
RT   "Isolation and sequence of the canine pancreatic phospholipase A2
RT   gene.";
RL   Eur. J. Biochem. 190:299-304(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=87175472; PubMed=3562437;
RA   Kerfelec B., Laforge K.S., Puigserver A., Scheele G.A.;
RT   "Primary structures of canine pancreatic lipase and phospholipase A2
RT   messenger RNAs.";
RL   Pancreas 1:430-437(1986).
CC   -!- FUNCTION: PA2 catalyzes the calcium-dependent hydrolysis of the 2-
CC       acyl groups in 3-sn-phosphoglycerides.
CC   -!- CATALYTIC ACTIVITY: Phosphatidylcholine + H(2)O = 1-
CC       acylglycerophosphocholine + a carboxylate.
CC   -!- COFACTOR: Binds 1 calcium ion per subunit (By similarity).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the phospholipase A2 family.
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DR   EMBL; D00035; BAA00023.1; -; mRNA.
DR   EMBL; M35301; AAA30883.1; -; mRNA.
DR   PIR; S11316; PSDG.
DR   RefSeq; NP_001003320.1; -.
DR   UniGene; Cfa.40293; -.
DR   HSSP; P00592; 1HN4.
DR   Ensembl; ENSCAFG00000010263; Canis familiaris.
DR   GeneID; 404011; -.
DR   KEGG; cfa:404011; -.
DR   HOVERGEN; P06596; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   InterPro; IPR016090; Phospholipase_A2.
DR   InterPro; IPR013090; Phospholipase_A2_AS.
DR   InterPro; IPR001211; Phospholipase_A2_euk.
DR   Gene3D; G3DSA:1.20.90.10; Phospholipase_A2; 1.
DR   PANTHER; PTHR11716; Phospholipase_A2; 1.
DR   Pfam; PF00068; Phospholip_A2_1; 1.
DR   PRINTS; PR00389; PHPHLIPASEA2.
DR   ProDom; PD000303; PhospholipaseA2; 1.
DR   SMART; SM00085; PA2c; 1.
DR   PROSITE; PS00119; PA2_ASP; 1.
DR   PROSITE; PS00118; PA2_HIS; 1.
PE   2: Evidence at transcript level;
KW   Calcium; Hydrolase; Lipid degradation; Metal-binding; Secreted;
KW   Signal.
FT   SIGNAL        1     15
FT   PROPEP       16     22
FT                                /FTId=PRO_0000022733.
FT   CHAIN        23    146       Phospholipase A2.
FT                                /FTId=PRO_0000022734.
FT   ACT_SITE     70     70       By similarity.
FT   ACT_SITE    121    121       By similarity.
FT   METAL        50     50       Calcium; via carbonyl oxygen (By
FT                                similarity).
FT   METAL        52     52       Calcium; via carbonyl oxygen (By
FT                                similarity).
FT   METAL        54     54       Calcium; via carbonyl oxygen (By
FT                                similarity).
FT   METAL        71     71       Calcium (By similarity).
FT   DISULFID     33     99       By similarity.
FT   DISULFID     49    146       By similarity.
FT   DISULFID     51     67       By similarity.
FT   DISULFID     66    127       By similarity.
FT   DISULFID     73    120       By similarity.
FT   DISULFID     83    113       By similarity.
FT   DISULFID    106    118       By similarity.
SQ   SEQUENCE   146 AA;  16236 MW;  F6258ED9527F3692 CRC64;
     MKFLVLAALL TVAAAEGGIS PRAVWQFRNM IKCTIPESDP LKDYNDYGCY CGLGGSGTPV
     DELDKCCQTH DHCYSEAKKL DSCKFLLDNP YTKIYSYSCS GSEITCSSKN KDCQAFICNC
     DRSAAICFSK APYNKEHKNL DTKKYC
//