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UniProtKB/Swiss-Prot entry P06572

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ERMM_STAEP
Primary accession number P06572
Secondary accession numbers None
Integrated into Swiss-Prot on January 1, 1988
Sequence was last modified on January 1, 1988 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 52)
Name and origin of the protein
Protein name rRNA adenine N-6-methyltransferase
Synonyms EC 2.1.1.48
Macrolide-lincosamide-streptogramin B resistance protein
Erythromycin resistance protein
Gene name
Name: ermM
From
Staphylococcus epidermidis [TaxID: 1282] 
Encoded on Plasmid pNE131.
Taxonomy Bacteria; Firmicutes; Bacillales; Staphylococcus.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=3091582 [NCBI, ExPASy, EBI, Israel, Japan]
Lampson B.C., Parisi J.T.;
"Nucleotide sequence of the constitutive macrolide-lincosamide-streptogramin B resistance plasmid pNE131 from Staphylococcus epidermidis and homologies with Staphylococcus aureus plasmids pE194 and pSN2.";
J. Bacteriol. 167:888-892(1986).
[2]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=3084450 [NCBI, ExPASy, EBI, Israel, Japan]
Lampson B.C., Parisi J.T.;
"Naturally occurring Staphylococcus epidermidis plasmid expressing constitutive macrolide-lincosamide-streptogramin B resistance contains a deleted attenuator.";
J. Bacteriol. 166:479-483(1986).
Comments
  • FUNCTION: This protein produces a dimethylation of the adenine residue at position 2058 in 23S rRNA, resulting in reduced affinity between ribosomes and macrolide-lincosamide-streptogramin B antibiotics.
  • CATALYTIC ACTIVITY: S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N6-methyladenine.
  • SIMILARITY: Belongs to the rRNA adenine N(6)-methyltransferase family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M12730; AAA98296.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A24497; A24497.
3D structure databases
HSSP P13956; 1QAN. [HSSP ENTRY / PDB]
SMR P06572; 10-244.
ModBase P06572.
Ontologies
GO
GO:0003723; Molecular function: RNA binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008988; Molecular function: rRNA (adenine-N6-)-methyltransferase activity (inferred from electronic annotation from EC).
GO:0046677; Biological process: response to antibiotic (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR001737; RRNA_meth_trans.
Graphical view of domain structure.
PANTHER PTHR11727; RRNA_meth_trans; 1.
Pfam PF00398; RrnaAD; 1.
Pfam graphical view of domain structure.
SMART SM00650; rADc; 1.
SMART graphical view of domain structure.
PROSITE PS01131; RRNA_A_DIMETH; 1.
BLOCKS P06572.
ProtoNet P06572.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Antibiotic resistance; Methyltransferase; Plasmid; RNA-binding; S-adenosyl-L-methionine; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   244  244     rRNA adenine N-6-methyltransferase. PRO_0000101685
BINDING   11    11        S-adenosyl-L-methionine; via carbonyl oxygen (By similarity). 
BINDING   13    13        S-adenosyl-L-methionine; via amide nitrogen (By similarity). 
BINDING   38    38        S-adenosyl-L-methionine; via carbonyl oxygen (By similarity). 
BINDING   59    59        S-adenosyl-L-methionine (By similarity). 
BINDING   84    84        S-adenosyl-L-methionine (By similarity). 
BINDING   101   101        S-adenosyl-L-methionine (By similarity). 
Sequence information
Length: 244 AA [This is the length of the unprocessed precursor] Molecular weight: 28941 Da [This is the MW of the unprocessed precursor] CRC64: 765404D860B6D620 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MNEKNIKHSQ NFITSKHNID KIMTNIRLNE HDNIFEIGSG KGHFTLELVQ RCNFVTAIEI 

        70         80         90        100        110        120 
DHKLCKTTEN KLVDHDNFQV LNKDILQFKF PKNQSYKIFG NIPYNISTDI IRKIVFDSIA 

       130        140        150        160        170        180 
DEIYLIVEYG FAKRLLNTKR SFALFLMAEV DISILSMVPR EYFHPKPKVN SSLIRLNRKK 

       190        200        210        220        230        240 
SRISHKDKQK YNYFVMKWVN KEYKKIFTKN QFNNSLKHAG IDDLNNISFE QFLSLFNSYK 


LFNK
P06572 in FASTA format

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