[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=R; DOI=10.1093/nar/13.18.6403; PubMed=2997708 [NCBI, ExPASy, EBI, Israel, Japan] Kiss A., Posfai G., Keller C.C., Venetianer P., Roberts R.J.; "Nucleotide sequence of the BsuRI restriction-modification system."; Nucleic Acids Res. 13:6403-6421(1985).

Comments

FUNCTION: This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the BsuRI endonuclease.
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
SUBUNIT: Monomer.
SIMILARITY: Belongs to the C5-methyltransferase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X02988; CAA26731.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

B23488; XYBSR1.

3D structure databases

HSSP

P20589; 1DCT. [HSSP ENTRY / PDB]

ModBase

P06530.

Protein family/group databases

REBASE

3340; M.BsuRI.

Ontologies

GO:0003886;	Molecular function: DNA (cytosine-5-)-methyltransferase activity (inferred from electronic annotation from EC).
GO:0009307;	Biological process: DNA restriction-modification system (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001525; C5_DNA_meth.
Graphical view of domain structure.

PANTHER

PTHR10629; C5_DNA_meth; 1.

Pfam

PF00145; DNA_methylase; 1.
Pfam graphical view of domain structure.

PRINTS

PR00105; C5METTRFRASE.

TIGRFAMs

TIGR00675; dcm; 1.

PROSITE

PS00094; C5_MTASE_1; 1.
PS00095; C5_MTASE_2; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Methyltransferase; Restriction system; S-adenosyl-L-methionine; Transferase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 436`	`436`	`Modification methylase BsuRI.`	`PRO_0000087864`
`ACT_SITE`	`157 157`		`By similarity.`

Sequence information

Length: 436 AA [This is the length of the unprocessed precursor]

Molecular weight: 49635 Da [This is the MW of the unprocessed precursor]

CRC64: 07EE0B2CA9140B19 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTLKIDIKGR GKYKPASDYS IDDVKNVLME KIFEESSRII NSDDDLEIIE KVDFRTDKIN 

        70         80         90        100        110        120 
VLSLFSGCGG LDLGFELAGL AAVIGEQAAM EAFKDKDRFN ELRNKSIFHT IYTNDLFKEA 

       130        140        150        160        170        180 
NQTYKTNFPG HVIQHEKDIR QVKYFPKCNL ILGGFPCPGF SEAGPRLIDD DRNFLYLHFI 

       190        200        210        220        230        240 
RSLIQAQPEI FVAENVKGMM TLGKGEVLNQ IIEDFASAGY RVQFKLLNAR DYGVPQLRER 

       250        260        270        280        290        300 
VIIEGVRKDI SFNYKYPSPT HGEETGLKPF KTLRDSIGDL VTDPGPYFTG SYSSIYMSRN 

       310        320        330        340        350        360 
RKKSWDEQSF TIQASGRQAP LHPGGLSMKK IGKDKWVFPD GEENHRRLSV KEIARVQTFP 

       370        380        390        400        410        420 
DWFQFSQGTN SQTSINNRLD KQYKQIGNAV PVLLAKAVAS PIANWAINYL ESSPNNKIKN 

       430 
RERKLSIRTF LRIKTS

P06530 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools