ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry P06168


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name ILV5_YEAST
Primary accession number P06168
Secondary accession numbers None
Integrated into Swiss-Prot on January 1, 1988
Sequence was last modified on January 1, 1988 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 87)
Name and origin of the protein
Protein name Ketol-acid reductoisomerase, mitochondrial [Precursor]
Synonyms EC 1.1.1.86
Acetohydroxy-acid reductoisomerase
Alpha-keto-beta-hydroxylacil reductoisomerase
Gene name
Name: ILV5
OrderedLocusNames: YLR355C
ORFNames: L9638.7
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1093/nar/14.24.9631; PubMed=3027658 [NCBI, ExPASy, EBI, Israel, Japan]
Petersen J.G.L., Holmberg S.;
"The ILV5 gene of Saccharomyces cerevisiae is highly expressed.";
Nucleic Acids Res. 14:9631-9651(1986).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204511 / S288c / AB972;
PubMed=9169871 [NCBI, ExPASy, EBI, Israel, Japan]
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
Nature 387:87-90(1997).
[3]
PROTEIN SEQUENCE OF 166-174.
STRAIN=ATCC 204508 / S288c;
PubMed=7895733 [NCBI, ExPASy, EBI, Israel, Japan]
Garrels J.I., Futcher B., Kobayashi R., Latter G.I., Schwender B., Volpe T., Warner J.R., McLaughlin C.S.;
"Protein identifications for a Saccharomyces cerevisiae protein database.";
Electrophoresis 15:1466-1486(1994).
[4]
IDENTIFICATION OF PROBABLE N-TERMINUS.
DOI=10.1073/pnas.93.25.14440; PubMed=8962070 [NCBI, ExPASy, EBI, Israel, Japan]
Shevchenko A., Jensen O.N., Podtelejnikov A.V., Sagliocco F., Wilm M., Vorm O., Mortensen P., Shevchenko A., Boucherie H., Mann M.;
"Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels.";
Proc. Natl. Acad. Sci. U.S.A. 93:14440-14445(1996).
[5]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-85; SER-317; THR-318 AND SER-355, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X04969; CAA28643.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U19102; AAB67753.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A24709; A24709.
RefSeq NP_013459.1; -.
3D structure databases
HSSP Q01292; 1QMG. [HSSP ENTRY / PDB]
ModBase P06168.
Protein-protein interaction databases
DIP DIP:6463N; -.
IntAct P06168; -.
2D gel databases
SWISS-2DPAGE P06168; -.
Organism-specific databases
CYGD YLR355c; -.
SGD S000004347; ILV5.
Yeast-GFP YLR355C.
Gene expression databases
ArrayExpress P06168; -.
GermOnline YLR355C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0042645; Cellular component: mitochondrial nucleoid (inferred from direct assay from SGD).
GO:0004455; Molecular function: ketol-acid reductoisomerase activity (traceable author statement from SGD).
GO:0009082; Biological process: branched chain family amino acid biosynthetic process (traceable author statement from SGD).
GO:0000002; Biological process: mitochondrial genome maintenance (inferred from mutant phenotype from SGD).
QuickGo view.
Family and domain databases
InterPro IPR013023; AcH_isomrdctse.
IPR000506; AcH_isomrdctse_C.
IPR013328; DHase_multihelical.
IPR013116; IlvN.
IPR016207; KetolA_reductoisomerase_fun.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
G3DSA:1.10.1040.10; Opine_DH; 1.
PANTHER PTHR21371; AcH_isomrdctse; 1.
Pfam PF01450; IlvC; 1.
PF07991; IlvN; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000119; Ilv5_fungal; 1.
TIGRFAMs TIGR00465; ilvC; 1.
BLOCKS P06168.
Proteomic databases
PeptideAtlas P06168; -.
Genome annotation databases
Ensembl YLR355C; Saccharomyces cerevisiae. [Contig view]
GeneID 851069; -.
GenomeReviews Y13138_GR; YLR355C.
KEGG sce:YLR355C; -.
NMPDR fig|4932.3.peg.4481; -.
Phylogenomic databases
HOGENOM P06168; -.
Other
LinkHub P06168; -.
ProtoNet P06168.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Direct protein sequencing; Magnesium; Mitochondrion; NADP; Oxidoreductase; Phosphoprotein; Transit peptide.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
TRANSIT   1    47  47     Mitochondrion (Potential). 
CHAIN   48   395  348     Ketol-acid reductoisomerase, mitochondrial. PRO_0000015634
NP_BIND   84    93  10     NADP (Potential). 
REGION   363   395  33     Hydrophilic. 
ACT_SITE   171   171        Potential. 
MOD_RES   85    85        Phosphotyrosine. 
MOD_RES   317   317        Phosphoserine. 
MOD_RES   318   318        Phosphothreonine. 
MOD_RES   355   355        Phosphoserine. 
Sequence information
Length: 395 AA [This is the length of the unprocessed precursor] Molecular weight: 44368 Da [This is the MW of the unprocessed precursor] CRC64: D76419A6AD68E85E [This is a checksum on the sequence]
        10         20         30         40         50         60 
MLRTQAARLI CNSRVITAKR TFALATRAAA YSRPAARFVK PMITTRGLKQ INFGGTVETV 

        70         80         90        100        110        120 
YERADWPREK LLDYFKNDTF ALIGYGSQGY GQGLNLRDNG LNVIIGVRKD GASWKAAIED 

       130        140        150        160        170        180 
GWVPGKNLFT VEDAIKRGSY VMNLLSDAAQ SETWPAIKPL LTKGKTLYFS HGFSPVFKDL 

       190        200        210        220        230        240 
THVEPPKDLD VILVAPKGSG RTVRSLFKEG RGINSSYAVW NDVTGKAHEK AQALAVAIGS 

       250        260        270        280        290        300 
GYVYQTTFER EVNSDLYGER GCLMGGIHGM FLAQYDVLRE NGHSPSEAFN ETVEEATQSL 

       310        320        330        340        350        360 
YPLIGKYGMD YMYDACSTTA RRGALDWYPI FKNALKPVFQ DLYESTKNGT ETKRSLEFNS 

       370        380        390 
QPDYREKLEK ELDTIRNMEI WKVGKEVRKL RPENQ 

P06168 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by ch flag SIB Switzerland Mirror sites: Australia  Brazil  Canada  China  Korea
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!