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UniProtKB/Swiss-Prot entry P06151

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LDHA_MOUSE
Primary accession number P06151
Secondary accession numbers None
Integrated into Swiss-Prot on January 1, 1988
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    July 22, 2008 (Entry version 90)
Name and origin of the protein
Protein name L-lactate dehydrogenase A chain
Synonyms LDH-A
EC 1.1.1.27
LDH muscle subunit
LDH-M
Gene name
Name: Ldha
Synonyms: Ldh-1, Ldh1
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=C57BL/10;
TISSUE=Liver;
PubMed=3036647 [NCBI, ExPASy, EBI, Israel, Japan]
Fukasawa K.M., Li S.S.-L.;
"Complete nucleotide sequence of the mouse lactate dehydrogenase-A functional gene: comparison of the exon-intron organization of dehydrogenase genes.";
Genetics 116:99-105(1987).
[2]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=3996406 [NCBI, ExPASy, EBI, Israel, Japan]
Li S.S.-L., Tiano H.F., Fukasawa K.M., Yagi K., Shimizu M., Sharief F.S., Nakashima Y., Pan Y.E.;
"Protein structure and gene organization of mouse lactate dehydrogenase-A isozyme.";
Eur. J. Biochem. 149:215-225(1985).
[3]
 NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=DBA/2J;
Hiraoka Y., Li S.S.-L.;
"Lactate dehydrogenase-A mRNAs in mouse testis and somatic tissues containing different 5' noncoding sequences.";
J. Genet. Mol. Biol. 1:1-6(1990).
[4]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
PubMed=3017306 [NCBI, ExPASy, EBI, Israel, Japan]
Fukasawa K.M., Li S.S.-L.;
"Nucleotide sequence of the putative regulatory region of mouse lactate dehydrogenase-A gene.";
Biochem. J. 235:435-439(1986).
[5]
 PROTEIN SEQUENCE OF 6-14; 43-57 AND 60-73, AND MASS SPECTROMETRY.
TISSUE=Hippocampus;
Lubec G., Klug S., Yang J.W., Zigmond M.;
Submitted (JUL-2007) to UniProtKB.
[6]
 PROTEIN SEQUENCE OF 43-57; 82-90 AND 306-315, AND MASS SPECTROMETRY.
STRAIN=C57BL/6;
TISSUE=Brain;
Lubec G., Kang S.U.;
Submitted (APR-2007) to UniProtKB.
[7]
 NUCLEOTIDE SEQUENCE [MRNA] OF 202-332.
DOI=10.1016/0020-711X(85)90298-8; PubMed=2993055 [NCBI, ExPASy, EBI, Israel, Japan]
Akai K., Yagi K., Tiano H.F., Pan Y.-C.E., Shimizu M., Fong K., Jungmann R.A., Li S.S.-L.;
"Isolation and characterization of a cDNA and a pseudogene for mouse lactate dehydrogenase-A isozyme.";
Int. J. Biochem. 17:645-648(1985).
[8]
 PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-239, AND MASS SPECTROMETRY.
TISSUE=Mast cell;
PubMed=17947660 [NCBI, ExPASy, EBI, Israel, Japan]
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.;
"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling.";
J. Immunol. 179:5864-5876(2007).
[9]
 PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-239, AND MASS SPECTROMETRY.
TISSUE=Brain;
DOI=10.1021/pr0701254; PubMed=18034455 [NCBI, ExPASy, EBI, Israel, Japan]
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.;
"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain.";
J. Proteome Res. 7:311-318(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Y00309; CAA68410.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X02520; CAA26360.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X02521; CAA26360.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X02522; CAA26360.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X02523; CAA26360.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X02524; CAA26360.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X02525; CAA26360.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X02526; CAA26360.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U13687; AAA21466.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X03753; CAA27387.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M17516; AAA39424.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A25205; DEMSLM.
I48240; I48240.
RefSeq NP_034829.1; -.
UniGene Mm.26751
3D structure databases
HSSP P00338; 1I10. [HSSP ENTRY / PDB]
SMR P06151; 2-332.
ModBase P06151.
Protein-protein interaction databases
IntAct P06151; -.
PTM databases
PhosphoSite P06151; -.
2D gel databases
SWISS-2DPAGE P06151; -.
REPRODUCTION-2DPAGE P06151; -.
Organism-specific databases
MGI MGI:96759; Ldha.
Gene expression databases
ArrayExpress P06151; -.
CleanEx MM_LDHA; -.
GermOnline ENSMUSG00000063229; Mus musculus.
Ontologies
GO
GO:0005829; Cellular component: cytosol (inferred from direct assay from MGI).
GO:0004459; Molecular function: L-lactate dehydrogenase activity (inferred from direct assay from MGI).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR001557; L-lactate/malate_DHase.
IPR011304; L-lactate_DHase.
IPR001236; Lactate/malate_DHase.
IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
Graphical view of domain structure.
Gene3D G3DSA:3.90.110.10; lact_mal_DH; 1.
Pfam PF02866; Ldh_1_C; 1.
PF00056; Ldh_1_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000102; Lac_mal_DH; 1.
PRINTS PR00086; LLDHDRGNASE.
TIGRFAMs TIGR01771; L-LDH-NAD; 1.
PROSITE PS00064; L_LDH; 1.
BLOCKS P06151.
Genome annotation databases
Ensembl ENSMUSG00000063229; Mus musculus. [Contig view]
GeneID 16828; -.
KEGG mmu:16828; -.
Phylogenomic databases
HOGENOM P06151; -.
HOVERGEN P06151; -.
Other
SOURCE Ldha; Mus musculus.
ProtoNet P06151.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Acetylation; Cytoplasm; Direct protein sequencing; Glycolysis; NAD; Oxidoreductase; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   332  331     L-lactate dehydrogenase A chain. PRO_0000168414
NP_BIND   29    57  29     NAD (By similarity). 
ACT_SITE   193   193        Proton acceptor (By similarity). 
BINDING   99    99        NAD (By similarity). 
BINDING   106   106        Substrate (By similarity). 
BINDING   138   138        NAD or substrate (By similarity). 
BINDING   169   169        Substrate (By similarity). 
BINDING   248   248        Substrate (By similarity). 
MOD_RES   2     2        N-acetylalanine (By similarity). 
MOD_RES   239   239        Phosphotyrosine. 
CONFLICT   11    11        N -> I (in Ref. 2). 
Sequence information
Length: 332 AA [This is the length of the unprocessed precursor] Molecular weight: 36499 Da [This is the MW of the unprocessed precursor] CRC64: 5AEB41E1E0B95100 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVMEDKLKG 

        70         80         90        100        110        120 
EMMDLQHGSL FLKTPKIVSS KDYCVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI 

       130        140        150        160        170        180 
IPNIVKYSPH CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV 

       190        200        210        220        230        240 
HALSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPELGT DADKEQWKEV HKQVVDSAYE 

       250        260        270        280        290        300 
VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGI 

       310        320        330 
SDVVKVTLTP EEEARLKKSA DTLWGIQKEL QF
P06151 in FASTA format

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