NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-22.
STRAIN=JF-1;
PubMed=3531194 [NCBI, ExPASy, EBI, Israel, Japan]
Shuber A.P., Orr E.C., Recny M.A., Schendel P.F., May H.D., Schauer N.L., Ferry J.G.;
"Cloning, expression, and nucleotide sequence of the formate dehydrogenase genes from Methanobacterium formicicum.";
J. Biol. Chem. 261:12942-12947(1986).

Comments

FUNCTION: Catalyzes the oxidation of formate.
CATALYTIC ACTIVITY: Formate + NAD⁺ = CO₂ + NADH.
COFACTOR: Binds 2 4Fe-4S clusters (Probable).
SUBUNIT: Dimer of alpha and beta chains.
SIMILARITY: Belongs to the frhB family.
SIMILARITY: Contains 2 4Fe-4S ferredoxin-type domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

J02581; AAA72183.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

B24698; B24698.

3D structure databases

ModBase

P06130.

Ontologies

GO:0051539;	Molecular function: 4 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008863;	Molecular function: formate dehydrogenase activity (inferred from electronic annotation from EC).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0022900;	Biological process: electron transport chain (inferred from electronic annotation from UniProtKB-KW).
GO:0006810;	Biological process: transport (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001450; 4Fe4S_Fe_S_bd.
IPR007516; Co_F420_Hydgase/DHase_bsu_N.
IPR007525; FrhB_FdhB_C.
IPR012285; Fum_reductase_C.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.1060.10; Fum_reductase_C; 1.

Pfam

PF00037; Fer4; 1.
PF04432; FrhB_FdhB_C; 1.
PF04422; FrhB_FdhB_N; 1.
Pfam graphical view of domain structure.

PROSITE

PS00198; 4FE4S_FER_1; 2.
PS51379; 4FE4S_FER_2; 2.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Direct protein sequencing; Electron transport; Iron; Iron-sulfur; Metal-binding; NAD; Oxidoreductase; Repeat; Transport.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 399`	`399`	`Formate dehydrogenase beta chain.`	`PRO_0000159222`
`DOMAIN`	`287 316`	`30`	`4Fe-4S ferredoxin-type 1.`
`DOMAIN`	`339 367`	`29`	`4Fe-4S ferredoxin-type 2.`
`METAL`	`296 296`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`299 299`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`302 302`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`306 306`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`348 348`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`351 351`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`354 354`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`358 358`		`Iron-sulfur (4Fe-4S) (By similarity).`

Sequence information

Length: 399 AA [This is the length of the unprocessed precursor]

Molecular weight: 43922 Da [This is the MW of the unprocessed precursor]

CRC64: D57F778C6C8F67BB [This is a checksum on the sequence]

        10         20         30         40         50         60 
MINTNDMFYA KSSDAEIAEA GEYGGAVTTL LKFLLKEGIV DAVLAVDSSA DLYDVVPILI 

        70         80         90        100        110        120 
EDPEDVVKAA GSLHFGTLNL AKVVTRYLDG AQDMKIAVTV KPCDAMTMVE LMKREKVNAD 

       130        140        150        160        170        180 
NVIMVGLNCG GTMPPVKGRQ MMEEFYEVDP DSVVKEEIAK GKLIVETEDG TEKEIPIDEL 

       190        200        210        220        230        240 
EDEGFGRRTN CRRCEVNIPR MADLACGNWG VIGPLAGKAT FIEVCSPKGA EVLEKAKEAG 

       250        260        270        280        290        300 
VIDLEDPIPK GIEIREKIDG AMVKLADKWQ GNDWEDKAGR EIFSVLTEYM DDFSRCLKCY 

       310        320        330        340        350        360 
GCREACPICY CEDCCLEANN GPDWLSKGEI PPSPMFHLER MLHMVESCTN CGQCEEVCPG 

       370        380        390 
EIPLAKIWHE VNAKMKDTFG YVKGTGDEKP PIAYFPVGK

P06130 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools