[1]	NUCLEOTIDE SEQUENCE [MRNA]. DOI=10.1016/0014-5793(90)81142-B; PubMed=2387396 [NCBI, ExPASy, EBI, Israel, Japan] Loennerdal B., Bergstroem S., Andersson Y., Hjalmarsson K., Sundqvist A.K., Hernell O.; "Cloning and sequencing of a cDNA encoding human milk beta-casein."; FEBS Lett. 269:153-156(1990).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. TISSUE=Placenta; DOI=10.1016/0378-1119(94)90754-4; PubMed=8112603 [NCBI, ExPASy, EBI, Israel, Japan] Hansson L., Edlund A., Johansson T., Hernell O., Stroemqvist M., Lindquist S., Loennerdal B., Bergstroem S.; "Structure of the human beta-casein encoding gene."; Gene 139:193-199(1994).
[3]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Mammary gland; Menon R.S.; Submitted (OCT-1989) to the EMBL/GenBank/DDBJ databases.
[4]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. TISSUE=Placenta; Kwiatkowski D.J.; "A high resolution linkage map of human 9q34.1."; Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
[5]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[6]	PROTEIN SEQUENCE OF 16-226, AND PHOSPHORYLATION AT THR-18; SER-21; SER-23; SER-24 AND SER-25. PubMed=6715339 [NCBI, ExPASy, EBI, Israel, Japan] Greenberg R., Groves M.L., Dower H.J.; "Human beta-casein. Amino acid sequence and identification of phosphorylation sites."; J. Biol. Chem. 259:5132-5138(1984).
[7]	PROTEIN SEQUENCE OF 18-33, PHOSPHORYLATION AT THR-18; SER-21; SER-23; SER-24 AND SER-25, AND MASS SPECTROMETRY. DOI=10.1021/pr800387s; PubMed=18847231 [NCBI, ExPASy, EBI, Israel, Japan] Poth A.G., Deeth H.C., Alewood P.F., Holland J.W.; "Analysis of the human casein phosphoproteome by 2-D electrophoresis and MALDI-TOF/TOF MS reveals new phosphoforms."; J. Proteome Res. 7:5017-5027(2008).
[8]	NUCLEOTIDE SEQUENCE [MRNA] OF 161-226. TISSUE=Mammary gland; DOI=10.1093/nar/17.7.2869; PubMed=2717418 [NCBI, ExPASy, EBI, Israel, Japan] Menon R.S., Ham R.G.; "Human beta-casein: partial cDNA sequence and apparent polymorphism."; Nucleic Acids Res. 17:2869-2869(1989).
[9]	PROTEIN SEQUENCE OF 176-215. PubMed=1369431 [NCBI, ExPASy, EBI, Israel, Japan] Azuma N., Yamauchi K.; "Plasmin cleavage of human beta-casein."; Biosci. Biotechnol. Biochem. 56:1140-1141(1992).

Comments

FUNCTION: Important role in determination of the surface properties of the casein micelles.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Mammary gland specific. Secreted in milk.
PTM: Form 1-P is phosphorylated once; half of the molecules are phosphorylated on Ser-24, half on Ser-25.
SIMILARITY: Belongs to the beta-casein family.
WEB RESOURCE: Name=Protein Spotlight; Note=Of buttons, digestion and glue - Issue 16 of November 2001; URL="http://www.expasy.org/spotlight/back_issues/sptlt016.shtml";.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X55739; CAA39270.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF027807; AAC82978.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X17070; CAA34916.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC069554; AAH69554.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC096194; AAH96194.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC096195; AAH96195.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC096196; AAH96196.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC096197; AAH96197.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X13766; CAA32017.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

IPI00010849; -.

I53730; KBHU.

NP_001882.1; -.

3D structure databases

DisProt

DP00199; -.

ModBase

P05814.

Protein-protein interaction databases

DIP

DIP:528N; -.

PTM databases

PhosphoSite

P05814; -.

Enzyme and pathway databases

Pathway_Interaction_DB

ptp1bpathway; Signaling events mediated by PTP1B.

Organism-specific databases

GC04M070869; -.

HIX0031475; -.

HGNC:2447; CSN2.

115460; gene. [NCBI / EBI]

PharmGKB

PA26950; -.

Gene expression databases

P05814; -.

P05814; -.

HS_CSN2; -.

ENSG00000135222; Homo sapiens.

Ontologies

GO:0005576;	Cellular component: extracellular region (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005509;	Molecular function: calcium ion binding (traceable author statement from ProtInc).
GO:0004857;	Molecular function: enzyme inhibitor activity (traceable author statement from ProtInc).
GO:0005215;	Molecular function: transporter activity (inferred from electronic annotation from InterPro).
GO:0006816;	Biological process: calcium ion transport (traceable author statement from ProtInc).
QuickGo view.

Family and domain databases

InterPro

IPR001588; Casein.
IPR016345; Casein_beta.
Graphical view of domain structure.

Pfam

PF00363; Casein; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF002372; Beta-casein; 1.

PROSITE

PS00306; CASEIN_ALPHA_BETA; 1.

Proteomic databases

PRIDE

P05814; -.

Genome annotation databases

Ensembl

ENSG00000135222; Homo sapiens. [Contig view]

GeneID

1447; -.

KEGG

hsa:1447; -.

Phylogenomic databases

HOGENOM

P05814; -.

HOVERGEN

P05814; -.

OMA

P05814; VVPYPQR.

Other

5947; -.

P05814; -.

CSN2; Homo sapiens.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; Milk protein; Phosphoprotein; Secreted; Signal.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 15`	`15`
`CHAIN`	`16 226`	`211`	`Beta-casein.`	`PRO_0000004475`
`MOD_RES`	`18 18`		`Phosphothreonine; in form 5-P.`
`MOD_RES`	`21 21`		`Phosphoserine; in form 4-P and form 5-P.`
`MOD_RES`	`23 23`		`Phosphoserine; in form 3-P, form 4-P and form 5-P.`
`MOD_RES`	`24 24`		`Phosphoserine; in form 1-P, form 2-P, form 3-P, form 4-P and form 5-P.`
`MOD_RES`	`25 25`		`Phosphoserine; in form 1-P, form 2-P, form 3-P, form 4-P and form 5-P.`
`CONFLICT`	`30 30`		`T -> P (in Ref. 6; AA sequence).`
`CONFLICT`	`34 34`		`Missing (in Ref. 1; CAA39270).`
`CONFLICT`	`48 50`		`EDE -> TDQ (in Ref. 6; AA sequence).`
`CONFLICT`	`120 120`		`S -> Q (in Ref. 6; AA sequence).`
`CONFLICT`	`133 133`		`L -> V (in Ref. 3; CAA34916).`
`CONFLICT`	`140 140`		`H -> Q (in Ref. 3; CAA34916).`
`CONFLICT`	`149 149`		`L -> S (in Ref. 6; AA sequence).`
`CONFLICT`	`173 173`		`Q -> E (in Ref. 6; AA sequence).`
`CONFLICT`	`182 184`		`QVV -> EVL (in Ref. 6; AA sequence).`
`CONFLICT`	`188 188`		`Q -> V (in Ref. 6; AA sequence).`
`CONFLICT`	`207 207`		`T -> P (in Ref. 6; AA sequence).`
`CONFLICT`	`214 222`		`TQPLAPVHN -> PEPSTTZABH (in Ref. 6; AA sequence).`

Sequence information

Length: 226 AA [This is the length of the unprocessed precursor]

Molecular weight: 25382 Da [This is the MW of the unprocessed precursor]

CRC64: 2619C524EA1358E8 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKVLILACLV ALALARETIE SLSSSEESIT EYKQKVEKVK HEDQQQGEDE HQDKIYPSFQ 

        70         80         90        100        110        120 
PQPLIYPFVE PIPYGFLPQN ILPLAQPAVV LPVPQPEIME VPKAKDTVYT KGRVMPVLKS 

       130        140        150        160        170        180 
PTIPFFDPQI PKLTDLENLH LPLPLLQPLM QQVPQPIPQT LALPPQPLWS VPQPKVLPIP 

       190        200        210        220 
QQVVPYPQRA VPVQALLLNQ ELLLNPTHQI YPVTQPLAPV HNPISV

P05814 in FASTA format

View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools