ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry P05804


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name BGLR_ECOLI
Primary accession number P05804
Secondary accession numbers None
Integrated into Swiss-Prot on November 1, 1988
Sequence was last modified on October 1, 1994 (Sequence version 2)
Annotations were last modified on    September 2, 2008 (Entry version 75)
Name and origin of the protein
Protein name Beta-glucuronidase
Synonyms GUS
EC 3.2.1.31
Beta-D-glucuronoside glucuronosohydrolase
Gene name
Name: uidA
Synonyms: gurA, gusA
OrderedLocusNames: b1617, JW1609
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=K12;
PubMed=3534890 [NCBI, ExPASy, EBI, Israel, Japan]
Jefferson R.A., Burgess S.M., Hirsh D.;
"Beta-glucuronidase from Escherichia coli as a gene-fusion marker.";
Proc. Natl. Acad. Sci. U.S.A. 83:8447-8451(1986).
[2]
SEQUENCE REVISION TO 279.
DOI=10.1016/0378-1119(94)90820-6; PubMed=8125312 [NCBI, ExPASy, EBI, Israel, Japan]
Schlaman H.R., Risseeuw E., Franke-Van Dijk M.E., Hooykaas P.J.;
"Nucleotide sequence corrections of the uidA open reading frame encoding beta-glucuronidase.";
Gene 138:259-260(1994).
[3]
SEQUENCE REVISION TO 420-425.
DOI=10.1007/BF00039422; PubMed=2103475 [NCBI, ExPASy, EBI, Israel, Japan]
Farrell L.B., Beachy R.N.;
"Manipulation of beta-glucuronidase for use as a reporter in vacuolar targeting studies.";
Plant Mol. Biol. 15:821-825(1990).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1093/dnares/3.6.363; PubMed=9097039 [NCBI, ExPASy, EBI, Israel, Japan]
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.;
"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.";
DNA Res. 3:363-377(1996).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[6]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M14641; AAA68923.1; ALT_SEQ; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S69414; AAB30197.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC74689.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAA15368.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR C64918; GBECGC.
RefSeq AP_002238.1; -.
NP_416134.1; -.
3D structure databases
HSSP P08236; 1BHG. [HSSP ENTRY / PDB]
ModBase P05804.
Protein-protein interaction databases
IntAct P05804; -.
Enzyme and pathway databases
BioCyc EcoCyc:BETA-GLUCURONID-MON; -.
MetaCyc:BETA-GLUCURONID-MON; -.
Organism-specific databases
EchoBASE EB1048; -.
EcoGene EG11055; uidA.
Ontologies
GO
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR006101; Glyco_hydro_2.
IPR013812; Glyco_hydro_2/20_Ig-like.
IPR006104; Glyco_hydro_2_carb-bd.
IPR006102; Glyco_hydro_2_Ig-like.
IPR006103; Glyco_hydro_2_TIM.
IPR013781; Glyco_hydro_sub_cat.
Graphical view of domain structure.
Gene3D G3DSA:2.60.40.320; Glyco_hydro_2/20_Ig-like; 1.
G3DSA:3.20.20.80; Glyco_hydro_cat; 1.
Pfam PF00703; Glyco_hydro_2; 1.
PF02836; Glyco_hydro_2_C; 1.
PF02837; Glyco_hydro_2_N; 1.
Pfam graphical view of domain structure.
PRINTS PR00132; GLHYDRLASE2.
PROSITE PS00719; GLYCOSYL_HYDROL_F2_1; 1.
PS00608; GLYCOSYL_HYDROL_F2_2; 1.
BLOCKS P05804.
Genome annotation databases
GeneID 946149; -.
GenomeReviews U00096_GR; b1617.
AP009048_GR; JW1609.
KEGG ecj:JW1609; -.
eco:b1617; -.
Phylogenomic databases
HOGENOM P05804; -.
Genome annotation databases
CMR P05804; b1617.
Other
ProtoNet P05804.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Glycosidase; Hydrolase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   603  603     Beta-glucuronidase. PRO_0000057680
ACT_SITE   413   413        Proton donor (By similarity). 
Sequence information
Length: 603 AA [This is the length of the unprocessed precursor] Molecular weight: 68447 Da [This is the MW of the unprocessed precursor] CRC64: E769C8D61A3B9A76 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MLRPVETPTR EIKKLDGLWA FSLDRENCGI DQRWWESALQ ESRAIAVPGS FNDQFADADI 

        70         80         90        100        110        120 
RNYAGNVWYQ REVFIPKGWA GQRIVLRFDA VTHYGKVWVN NQEVMEHQGG YTPFEADVTP 

       130        140        150        160        170        180 
YVIAGKSVRI TVCVNNELNW QTIPPGMVIT DENGKKKQSY FHDFFNYAGI HRSVMLYTTP 

       190        200        210        220        230        240 
NTWVDDITVV THVAQDCNHA SVDWQVVANG DVSVELRDAD QQVVATGQGT SGTLQVVNPH 

       250        260        270        280        290        300 
LWQPGEGYLY ELCVTAKSQT ECDIYPLRVG IRSVAVKGEQ FLINHKPFYF TGFGRHEDAD 

       310        320        330        340        350        360 
LRGKGFDNVL MVHDHALMDW IGANSYRTSH YPYAEEMLDW ADEHGIVVID ETAAVGFNLS 

       370        380        390        400        410        420 
LGIGFEAGNK PKELYSEEAV NGETQQAHLQ AIKELIARDK NHPSVVMWSI ANEPDTRPQG 

       430        440        450        460        470        480 
AREYFAPLAE ATRKLDPTRP ITCVNVMFCD AHTDTISDLF DVLCLNRYYG WYVQSGDLET 

       490        500        510        520        530        540 
AEKVLEKELL AWQEKLHQPI IITEYGVDTL AGLHSMYTDM WSEEYQCAWL DMYHRVFDRV 

       550        560        570        580        590        600 
SAVVGEQVWN FADFATSQGI LRVGGNKKGI FTRDRKPKSA AFLLQKRWTG MNFGEKPQQG 


GKQ 

P05804 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by ch flag SIB Switzerland Mirror sites: Australia  Brazil  Canada  China  Korea
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!