ID   PYRB_BACSU              Reviewed;         304 AA.
AC   P05654;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   22-JUL-2008, entry version 82.
DE   RecName: Full=Aspartate carbamoyltransferase;
DE            EC=2.1.3.2;
DE   AltName: Full=Aspartate transcarbamylase;
DE            Short=ATCase;
GN   Name=pyrB; OrderedLocusNames=BSU15490;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=86278215; PubMed=3015959;
RA   Lerner C.G., Switzer R.L.;
RT   "Cloning and structure of the Bacillus subtilis aspartate
RT   transcarbamylase gene (pyrB).";
RL   J. Biol. Chem. 261:11156-11165(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=91225016; PubMed=1709162;
RA   Quinn C.L., Stephenson B.T., Switzer R.L.;
RT   "Functional organization and nucleotide sequence of the Bacillus
RT   subtilis pyrimidine biosynthetic operon.";
RL   J. Biol. Chem. 266:9113-9127(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-303, AND MASS
RP   SPECTROMETRY.
RX   PubMed=17218307; DOI=10.1074/mcp.M600464-MCP200;
RA   Macek B., Mijakovic I., Olsen J.V., Gnad F., Kumar C., Jensen P.R.,
RA   Mann M.;
RT   "The serine/threonine/tyrosine phosphoproteome of the model bacterium
RT   Bacillus subtilis.";
RL   Mol. Cell. Proteomics 6:697-707(2007).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
RX   MEDLINE=91296766; PubMed=1906175;
RA   Stevens R.C., Reinisch K.M., Lipscomb W.N.;
RT   "Molecular structure of Bacillus subtilis aspartate transcarbamoylase
RT   at 3.0-A resolution.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:6087-6091(1991).
CC   -!- CATALYTIC ACTIVITY: Carbamoyl phosphate + L-aspartate = phosphate
CC       + N-carbamoyl-L-aspartate.
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo
CC       pathway; UMP from HCO(3)(-): step 2/6.
CC   -!- SUBUNIT: Homotrimer.
CC   -!- SIMILARITY: Belongs to the ATCase/OTCase family.
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DR   EMBL; M13128; AAA22685.1; -; Genomic_DNA.
DR   EMBL; M59757; AAA21267.1; -; Genomic_DNA.
DR   EMBL; Z99112; CAB13423.1; -; Genomic_DNA.
DR   PIR; A25015; OWBSAC.
DR   RefSeq; NP_389432.1; -.
DR   PDB; 2AT2; X-ray; 3.00 A; A/B/C=1-300.
DR   PDBsum; 2AT2; -.
DR   GeneID; 937734; -.
DR   GenomeReviews; AL009126_GR; BSU15490.
DR   KEGG; bsu:BSU15490; -.
DR   NMPDR; fig|224308.1.peg.1551; -.
DR   SubtiList; BG10713; pyrB.
DR   HOGENOM; P05654; -.
DR   BioCyc; BSUB224308:BSU1551-MON; -.
DR   LinkHub; P05654; -.
DR   GO; GO:0004070; F:aspartate carbamoyltransferase activity; IEA:HAMAP.
DR   GO; GO:0006221; P:pyrimidine nucleotide biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00001; -; 1.
DR   InterPro; IPR006130; Asp/Orn_carbamoyltranf.
DR   InterPro; IPR006132; Asp/Orn_carbamoyltranf_P_bd.
DR   InterPro; IPR006131; Asp_carbamoyltransf_Asp/Orn_bd.
DR   InterPro; IPR002082; Aspartate_carbamoyltransf_euk.
DR   Pfam; PF00185; OTCace; 1.
DR   Pfam; PF02729; OTCace_N; 1.
DR   PRINTS; PR00100; AOTCASE.
DR   PRINTS; PR00101; ATCASE.
DR   TIGRFAMs; TIGR00670; asp_carb_tr; 1.
DR   PROSITE; PS00097; CARBAMOYLTRANSFERASE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Phosphoprotein;
KW   Pyrimidine biosynthesis; Transferase.
FT   CHAIN         1    304       Aspartate carbamoyltransferase.
FT                                /FTId=PRO_0000113099.
FT   MOD_RES     303    303       Phosphoserine.
SQ   SEQUENCE   304 AA;  34171 MW;  375EA677346E15FE CRC64;
     MKHLTTMSEL STEEIKDLLQ TAQELKSGKT DNQLTGKFAA NLFFEPSTRT RFSFEVAEKK
     LGMNVLNLDG TSTSVQKGET LYDTIRTLES IGVDVCVIRH SEDEYYEELV SQVNIPILNA
     GDGCGQHPTQ SLLDLMTIYE EFNTFKGLTV SIHGDIKHSR VARSNAEVLT RLGARVLFSG
     PSEWQDEENT FGTYVSMDEA VESSDVVMLL RIQNERHQSA VSQEGYLNKY GLTVERAERM
     KRHAIIMHPA PVNRGVEIDD SLVESEKSRI FKQMKNGVFI RMAVIQCALQ TNVKRGEAAY
     VISH
//