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UniProtKB/Swiss-Prot entry P05458


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PTRA_ECOLI
Primary accession number P05458
Secondary accession numbers P78106 Q2MA16
Integrated into Swiss-Prot on November 1, 1988
Sequence was last modified on November 1, 1988 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 98)
Name and origin of the protein
Protein name Protease 3 [Precursor]
Synonyms EC 3.4.24.55
Protease III
Pitrilysin
Protease pi
Gene name
Name: ptrA
Synonyms: ptr
OrderedLocusNames: b2821, JW2789
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1093/nar/14.19.7695; PubMed=3534791 [NCBI, ExPASy, EBI, Israel, Japan]
Finch P.W., Wilson R.E., Brown K., Hickson I.D., Emmerson P.T.;
"Complete nucleotide sequence of the Escherichia coli ptr gene encoding protease III.";
Nucleic Acids Res. 14:7695-7703(1986).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 853-962.
DOI=10.1093/nar/14.21.8573; PubMed=3537960 [NCBI, ExPASy, EBI, Israel, Japan]
Finch P.W., Storey A., Chapman K.E., Brown K., Hickson I.D., Emmerson P.T.;
"Complete nucleotide sequence of the Escherichia coli recB gene.";
Nucleic Acids Res. 14:8573-8582(1986).
[5]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-296.
STRAIN=K12;
DOI=10.1016/0378-1119(87)90486-0; PubMed=3308636 [NCBI, ExPASy, EBI, Israel, Japan]
Claverie-Martin F., Diaz-Torres M.R., Kushner S.R.;
"Analysis of the regulatory region of the protease III (ptr) gene of Escherichia coli K-12.";
Gene 54:185-195(1987).
[6]
MUTAGENESIS, AND ACTIVE SITE.
PubMed=1570301 [NCBI, ExPASy, EBI, Israel, Japan]
Becker A.B., Roth R.A.;
"An unusual active site identified in a family of zinc metalloendopeptidases.";
Proc. Natl. Acad. Sci. U.S.A. 89:3835-3839(1992).
[7]
MUTAGENESIS, AND ACTIVE SITE.
PubMed=8099278 [NCBI, ExPASy, EBI, Israel, Japan]
Becker A.B., Roth R.A.;
"Identification of glutamate-169 as the third zinc-binding residue in proteinase III, a member of the family of insulin-degrading enzymes.";
Biochem. J. 292:137-142(1993).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X04581; CAA28249.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U29581; AAB40468.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC75860.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAE76890.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X06227; CAA29576.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M17095; AAA24436.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR F65064; SNECPI.
RefSeq AP_003384.1; -.
NP_417298.1; -.
3D structure databases
PDB
1Q2L; X-ray; 2.20 A; A=24-962.[ExPASy / RCSB / EBI]
PDBsum 1Q2L; -.
ModBase P05458.
Protein family/group databases
MEROPS M16.001; -.
Enzyme and pathway databases
BioCyc EcoCyc:EG10786-MON; -.
2D gel databases
ECO2DBASE G095.0; 6TH EDITION.
Organism-specific databases
EchoBASE EB0779; -.
EcoGene EG10786; ptrA.
Ontologies
GO
GO:0042597; Cellular component: periplasmic space (inferred from electronic annotation from UniProtKB-KW).
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR011237; Pept_M16_core.
IPR011765; Pept_M16_N.
IPR001431; Pept_M16_Zn_BS.
IPR007863; Peptidase_M16_C.
Graphical view of domain structure.
Gene3D G3DSA:3.30.830.10; Pept_M16_core; 1.
Pfam PF00675; Peptidase_M16; 1.
PF05193; Peptidase_M16_C; 2.
Pfam graphical view of domain structure.
PROSITE PS00143; INSULINASE; 1.
BLOCKS P05458.
ProtoNet P05458.
Genome annotation databases
GeneID 947284; -.
GenomeReviews U00096_GR; b2821.
AP009048_GR; JW2789.
KEGG ecj:JW2789; -.
eco:b2821; -.
Phylogenomic databases
HOGENOM P05458; -.
Genome annotation databases
CMR P05458; b2821.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Complete proteome; Hydrolase; Magnesium; Metal-binding; Metalloprotease; Periplasm; Protease; Signal; Zinc.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
SIGNAL   1    23  23      
CHAIN   24   962  939     Protease 3. PRO_0000026758
ACT_SITE   91    91        Proton acceptor. 
METAL   88    88        Zinc. 
METAL   92    92        Zinc. 
METAL   169   169        Zinc. 
MUTAGEN   88    88        H->R: Loss of activity and of Zn-binding. 
MUTAGEN   91    91        E->Q: Loss of activity. 
MUTAGEN   92    92        H->R: Loss of activity and of Zn-binding. 
MUTAGEN   162   162        E->Q: 20% loss of activity. 
MUTAGEN   169   169        E->Q: Loss of activity and of Zn-binding. 
MUTAGEN   204   204        E->Q: No loss of activity. 
CONFLICT   277   284        IIIHYVPA -> HYHSLRPW (in Ref. 5; AAA24436). 
STRAND   27    29  3      
STRAND   43    49  7      
STRAND   54    59  6      
STRAND   64    73  10      
HELIX   76    78  3      
HELIX   81    83  3      
HELIX   86    93  8      
STRAND   98   101  4      
HELIX   106   112  7      
TURN   113   115  3      
STRAND   117   122  6      
STRAND   127   133  7      
HELIX   135   137  3      
HELIX   138   150  13      
HELIX   159   173  15      
HELIX   177   187  11      
HELIX   194   196  3      
HELIX   203   206  4      
HELIX   214   225  12      
TURN   228   230  3      
STRAND   232   239  8      
HELIX   241   250  10      
HELIX   252   254  3      
HELIX   272   274  3      
STRAND   275   281  7      
STRAND   289   297  9      
HELIX   300   305  6      
HELIX   307   316  10      
HELIX   323   329  7      
STRAND   333   343  11      
STRAND   346   357  12      
HELIX   359   363  5      
HELIX   365   382  18      
HELIX   386   401  16      
HELIX   409   419  11      
HELIX   424   426  3      
TURN   427   432  6      
HELIX   439   448  10      
HELIX   451   453  3      
STRAND   455   459  5      
TURN   470   472  3      
STRAND   475   480  6      
HELIX   483   494  12      
STRAND   524   529  6      
STRAND   532   537  6      
STRAND   545   555  11      
HELIX   557   560  4      
HELIX   563   589  27      
STRAND   592   612  21      
HELIX   613   626  14      
HELIX   632   648  17      
HELIX   653   663  11      
STRAND   666   668  3      
HELIX   672   678  7      
HELIX   679   681  3      
HELIX   684   695  12      
STRAND   699   707  9      
HELIX   710   724  15      
STRAND   735   737  3      
STRAND   743   749  7      
STRAND   752   754  3      
STRAND   756   763  8      
HELIX   769   787  19      
HELIX   789   792  4      
STRAND   797   799  3      
STRAND   801   808  8      
STRAND   811   823  13      
HELIX   825   844  20      
HELIX   848   862  15      
HELIX   869   882  14      
HELIX   889   898  10      
HELIX   902   912  11      
STRAND   917   925  9      
TURN   930   932  3      
HELIX   947   951  5      
STRAND   956   958  3      
Sequence information
Length: 962 AA [This is the length of the unprocessed precursor] Molecular weight: 107708 Da [This is the MW of the unprocessed precursor] CRC64: 0558C68C2F1A0540 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MPRSTWFKAL LLLVALWAPL SQAETGWQPI QETIRKSDKD NRQYQAIRLD NGMVVLLVSD 

        70         80         90        100        110        120 
PQAVKSLSAL VVPVGSLEDP EAYQGLAHYL EHMSLMGSKK YPQADSLAEY LKMHGGSHNA 

       130        140        150        160        170        180 
STAPYRTAFY LEVENDALPG AVDRLADAIA EPLLDKKYAE RERNAVNAEL TMARTRDGMR 

       190        200        210        220        230        240 
MAQVSAETIN PAHPGSKFSG GNLETLSDKP GNPVQQALKD FHEKYYSANL MKAVIYSNKP 

       250        260        270        280        290        300 
LPELAKMAAD TFGRVPNKES KKPEITVPVV TDAQKGIIIH YVPALPRKVL RVEFRIDNNS 

       310        320        330        340        350        360 
AKFRSKTDEL ITYLIGNRSP GTLSDWLQKQ GLVEGISANS DPIVNGNSGV LAISASLTDK 

       370        380        390        400        410        420 
GLANRDQVVA AIFSYLNLLR EKGIDKQYFD ELANVLDIDF RYPSITRDMD YVEWLADTMI 

       430        440        450        460        470        480 
RVPVEHTLDA VNIADRYDAK AVKERLAMMT PQNARIWYIS PKEPHNKTAY FVDAPYQVDK 

       490        500        510        520        530        540 
ISAQTFADWQ KKAADIALSL PELNPYIPDD FSLIKSEKKY DHPELIVDES NLRVVYAPSR 

       550        560        570        580        590        600 
YFASEPKADV SLILRNPKAM DSARNQVMFA LNDYLAGLAL DQLSNQASVG GISFSTNANN 

       610        620        630        640        650        660 
GLMVNANGYT QRLPQLFQAL LEGYFSYTAT EDQLEQAKSW YNQMMDSAEK GKAFEQAIMP 

       670        680        690        700        710        720 
AQMLSQVPYF SRDERRKILP SITLKEVLAY RDALKSGARP EFMVIGNMTE AQATTLARDV 

       730        740        750        760        770        780 
QKQLGADGSE WCRNKDVVVD KKQSVIFEKA GNSTDSALAA VFVPTGYDEY TSSAYSSLLG 

       790        800        810        820        830        840 
QIVQPWFYNQ LRTEEQLGYA VFAFPMSVGR QWGMGFLLQS NDKQPSFLWE RYKAFFPTAE 

       850        860        870        880        890        900 
AKLRAMKPDE FAQIQQAVIT QMLQAPQTLG EEASKLSKDF DRGNMRFDSR DKIVAQIKLL 

       910        920        930        940        950        960 
TPQKLADFFH QAVVEPQGMA ILSQISGSQN GKAEYVHPEG WKVWENVSAL QQTMPLMSEK 


NE 

P05458 in FASTA format

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