NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=2820981 [NCBI, ExPASy, EBI, Israel, Japan]
Kurowski B., Ludwig B.;
"The genes of the Paracoccus denitrificans bc1 complex. Nucleotide sequence and homologies between bacterial and mitochondrial subunits.";
J. Biol. Chem. 262:13805-13811(1987).

[2]

PROTEIN SEQUENCE OF 17-23 AND 37-60.
PubMed=7601123 [NCBI, ExPASy, EBI, Israel, Japan]
Kleymann G., Iwata S., Wiesmueller K.-H., Ludwig B., Haase W., Michel H.;
"Immunoelectron microscopy and epitope mapping with monoclonal antibodies suggest the existence of an additional N-terminal transmembrane helix in the cytochrome b subunit of bacterial ubiquinol:cytochrome-c oxidoreductases.";
Eur. J. Biochem. 230:359-363(1995).

Comments

FUNCTION: Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
CATALYTIC ACTIVITY: QH₂ + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H⁺.
COFACTOR: Binds 1 2Fe-2S cluster per subunit (By similarity).
SUBUNIT: The main subunits of complex b-c1 are: cytochrome b, cytochrome c1 and the Rieske protein.
SUBCELLULAR LOCATION: Cell membrane; Single-pass membrane protein.
MISCELLANEOUS: The Rieske protein is a high potential 2Fe-2S protein.
SIMILARITY: Contains 1 Rieske domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M17522; AAA25571.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X05799; CAA29243.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A29413; A29413.

3D structure databases

HSSP

P13272; 1RIE. [HSSP ENTRY / PDB]

SMR

P05417; 13-190.

ModBase

P05417.

Ontologies

GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0022900;	Biological process: electron transport chain (inferred from electronic annotation from UniProtKB-KW).
GO:0006810;	Biological process: transport (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR014349; Rieske.
IPR005805; Rieske_C.
IPR006317; Rieske_proteo.
IPR005806; Rieske_reg.
IPR006311; Tat.
Graphical view of domain structure.

Gene3D

G3DSA:2.102.10.10; Rieske_reg; 1.

PANTHER

PTHR10134; Rieske; 1.

Pfam

PF00355; Rieske; 1.
Pfam graphical view of domain structure.

PRINTS

PR00162; RIESKE.

TIGRFAMs

TIGR01416; Rieske_proteo; 1.
TIGR01409; TAT_signal_seq; 1.

PROSITE

PS51296; RIESKE; 1.
PS51318; TAT; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

2Fe-2S; Cell membrane; Direct protein sequencing; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Oxidoreductase; Transmembrane; Transport.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 190`	`190`	`Ubiquinol-cytochrome c reductase iron-sulfur subunit.`	`PRO_0000127761`
`TRANSMEM`	`18 39`	`22`	`Potential.`
`DOMAIN`	`95 188`	`94`	`Rieske.`
`METAL`	`132 132`		`Iron-sulfur (2Fe-2S) (By similarity).`
`METAL`	`134 134`		`Iron-sulfur (2Fe-2S); via pros nitrogen (By similarity).`
`METAL`	`152 152`		`Iron-sulfur (2Fe-2S) (By similarity).`
`METAL`	`155 155`		`Iron-sulfur (2Fe-2S); via pros nitrogen (By similarity).`
`DISULFID`	`137 154`		`By similarity.`

Sequence information

Length: 190 AA [This is the length of the unprocessed precursor]

Molecular weight: 20300 Da [This is the MW of the unprocessed precursor]

CRC64: F83F5D9A9C1FBEE4 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSHADEHAGD HGATRRDFLY YATAGAGTVA AGAAAWTLVN QMNPSADVQA LASIQVDVSG 

        70         80         90        100        110        120 
VETGTQLTVK WLGKPVFIRR RTEDEIQAGR EVDLGQLIDR SAQNSNKPDA PATDENRTMD 

       130        140        150        160        170        180 
EAGEWLVMIG VCTHLGCVPI GDGAGDFGGW FCPCHGSHYD TSGRIRRGPA PQNLHIPVAE 

       190 
FLDDTTIKLG

P05417 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools