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UniProtKB/Swiss-Prot entry P05375


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PEM2_YEAST
Primary accession number P05375
Secondary accession numbers None
Integrated into Swiss-Prot on November 1, 1988
Sequence was last modified on November 1, 1988 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 71)
Name and origin of the protein
Protein name Methylene-fatty-acyl-phospholipid synthase
Synonyms EC 2.1.1.16
Unsaturated phospholipid methyltransferase
Overproducer of inositol protein 3
Gene name
Name: PEM2
Synonyms: OPI3
OrderedLocusNames: YJR073C
ORFNames: J1824
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=2445736 [NCBI, ExPASy, EBI, Israel, Japan]
Kodaki T., Yamashita S.;
"Yeast phosphatidylethanolamine methylation pathway. Cloning and characterization of two distinct methyltransferase genes.";
J. Biol. Chem. 262:15428-15435(1987).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
DOI=10.1002/(SICI)1097-0061(199607)12:9<869::AID-YEA964>3.3.CO;2-T; PubMed=8840504 [NCBI, ExPASy, EBI, Israel, Japan]
Huang M.-E., Manus V., Chuat J.-C., Galibert F.;
"Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI.";
Yeast 12:869-875(1996).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=8641269 [NCBI, ExPASy, EBI, Israel, Japan]
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
EMBO J. 15:2031-2049(1996).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
DOI=10.1101/gr.6037607; PubMed=17322287 [NCBI, ExPASy, EBI, Israel, Japan]
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.;
"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.";
Genome Res. 17:536-543(2007).
[5]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M16988; AAA34851.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z49573; CAA89601.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
L47993; AAB39298.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY557899; AAS56225.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR B28443; B28443.
RefSeq NP_012607.1; -.
3D structure databases
ModBase P05375.
Protein-protein interaction databases
DIP DIP:2888N; -.
IntAct P05375; -.
Organism-specific databases
CYGD YJR073c; -.
SGD S000003834; OPI3.
Yeast-GFP YJR073C.
Gene expression databases
ArrayExpress P05375; -.
GermOnline YJR073C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005739; Cellular component: mitochondrion (inferred from direct assay from SGD).
QuickGo view.
Family and domain databases
InterPro IPR007318; PEMT.
Graphical view of domain structure.
PANTHER PTHR15458; PEMT; 1.
Pfam PF04191; PEMT; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF005444; PEMT; 1.
BLOCKS P05375.
ProtoNet P05375.
Genome annotation databases
Ensembl YJR073C; Saccharomyces cerevisiae. [Contig view]
GeneID 853536; -.
GenomeReviews Y13136_GR; YJR073C.
KEGG sce:YJR073C; -.
NMPDR fig|4932.3.peg.3581; -.
Phylogenomic databases
HOGENOM P05375; -.
Other
LinkHub P05375; -.
NextBio 974242; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Membrane; Methyltransferase; Phospholipid biosynthesis; S-adenosyl-L-methionine; Transferase; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   206  206     Methylene-fatty-acyl-phospholipid synthase. PRO_0000193924
TRANSMEM   25    41  17     Potential. 
TRANSMEM   100   117  18     Potential. 
Sequence information
Length: 206 AA [This is the length of the unprocessed precursor] Molecular weight: 23150 Da [This is the MW of the unprocessed precursor] CRC64: BC0CDCC1C7B2AD36 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKESVQEIIQ QLIHSVDLQS SKFQLAIVCT MFNPIFWNIV ARMEYHKHSL TKMCGGARKG 

        70         80         90        100        110        120 
CYMLAATIFS LGIVRDMVYE SALREQPTCS LITGENWTKL GVALFGLGQV LVLSSMYKLG 

       130        140        150        160        170        180 
ITGTYLGDYF GILMDERVTG FPFNVSNNPM YQGSTLSFLG IALYKGKPAG LVVSAVVYFM 

       190        200 
YKIALRWEEP FTAMIYANRD KAKKNM 

P05375 in FASTA format

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BLAST logo BLAST submission on ExPASy/SIB
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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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