ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry P05374


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name PEM1_YEAST
Primary accession number P05374
Secondary accession numbers None
Integrated into Swiss-Prot on November 1, 1988
Sequence was last modified on November 1, 1988 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 62)
Name and origin of the protein
Protein name Phosphatidylethanolamine N-methyltransferase
Synonyms PEAMT
EC 2.1.1.17
Gene name
Name: PEM1
Synonyms: CHO2
OrderedLocusNames: YGR157W
ORFNames: G6673
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=2445736 [NCBI, ExPASy, EBI, Israel, Japan]
Kodaki T., Yamashita S.;
"Yeast phosphatidylethanolamine methylation pathway. Cloning and characterization of two distinct methyltransferase genes.";
J. Biol. Chem. 262:15428-15435(1987).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=8585325 [NCBI, ExPASy, EBI, Israel, Japan]
Skala J., Nawrocki A., Goffeau A.;
"The sequence of a 27 kb segment on the right arm of chromosome VII from Saccharomyces cerevisiae reveals MOL1, NAT2, RPL30B, RSR1, CYS4, PEM1/CHO2, NSR1 genes and ten new open reading frames.";
Yeast 11:1421-1427(1995).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
PubMed=9169869 [NCBI, ExPASy, EBI, Israel, Japan]
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
Nature 387:81-84(1997).
[4]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[5]
TOPOLOGY [LARGE SCALE ANALYSIS].
DOI=10.1073/pnas.0604075103; PubMed=16847258 [NCBI, ExPASy, EBI, Israel, Japan]
Kim H., Melen K., Oesterberg M., von Heijne G.;
"A global topology map of the Saccharomyces cerevisiae membrane proteome.";
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-582, AND MASS SPECTROMETRY.
DOI=10.1073/pnas.0701622104; PubMed=17563356 [NCBI, ExPASy, EBI, Israel, Japan]
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.;
"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases.";
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M16987; AAA34850.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X85807; CAA59814.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z72942; CAA97171.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A28443; A28443.
RefSeq NP_011673.1; -.
3D structure databases
ModBase P05374.
Protein-protein interaction databases
DIP DIP:8037N; -.
Organism-specific databases
CYGD YGR157w; -.
SGD S000003389; CHO2.
Yeast-GFP YGR157W.
Gene expression databases
ArrayExpress P05374; -.
GermOnline YGR157W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0004608; Molecular function: phosphatidylethanolamine N-methyltransferase activity (inferred from direct assay from SGD).
GO:0006656; Biological process: phosphatidylcholine biosynthetic process (inferred from direct assay from SGD).
QuickGo view.
Family and domain databases
InterPro IPR016219; Phosphatid-EA_MeTrfase_fun.
Graphical view of domain structure.
PIRSF PIRSF000383; PEAMT; 1.
BLOCKS P05374.
ProtoNet P05374.
Proteomic databases
PeptideAtlas P05374; -.
Genome annotation databases
Ensembl YGR157W; Saccharomyces cerevisiae. [Contig view]
GeneID 853061; -.
GenomeReviews Y13135_GR; YGR157W.
KEGG sce:YGR157W; -.
NMPDR fig|4932.3.peg.2793; -.
Phylogenomic databases
HOGENOM P05374; -.
Other
LinkHub P05374; -.
NextBio 972998; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Membrane; Methyltransferase; Phospholipid biosynthesis; Phosphoprotein; S-adenosyl-L-methionine; Transferase; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   869  869     Phosphatidylethanolamine N-methyltransferase. PRO_0000058304
TOPO_DOM   1    59  59     Cytoplasmic (Potential). 
TRANSMEM   60    80  21     Potential. 
TOPO_DOM   81    84  4     Extracellular (Potential). 
TRANSMEM   85   105  21     Potential. 
TOPO_DOM   106   178  73     Cytoplasmic (Potential). 
TRANSMEM   179   199  21     Potential. 
TOPO_DOM   200   212  13     Extracellular (Potential). 
TRANSMEM   213   233  21     Potential. 
TOPO_DOM   234   244  11     Cytoplasmic (Potential). 
TRANSMEM   245   265  21     Potential. 
TOPO_DOM   266   285  20     Extracellular (Potential). 
TRANSMEM   286   306  21     Potential. 
TOPO_DOM   307   365  59     Cytoplasmic (Potential). 
TRANSMEM   366   386  21     Potential. 
TOPO_DOM   387   390  4     Extracellular (Potential). 
TRANSMEM   391   411  21     Potential. 
TOPO_DOM   412   438  27     Cytoplasmic (Potential). 
TRANSMEM   439   459  21     Potential. 
TOPO_DOM   460   463  4     Extracellular (Potential). 
TRANSMEM   464   484  21     Potential. 
TOPO_DOM   485   495  11     Cytoplasmic (Potential). 
TRANSMEM   496   516  21     Potential. 
TOPO_DOM   517   542  26     Extracellular (Potential). 
TRANSMEM   543   563  21     Potential. 
TOPO_DOM   564   869  306     Cytoplasmic (Potential). 
MOD_RES   582   582        Phosphoserine. 
Sequence information
Length: 869 AA [This is the length of the unprocessed precursor] Molecular weight: 101204 Da [This is the MW of the unprocessed precursor] CRC64: A273F179B4E46A20 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSSCKTTLSE MVGSVTKDRG TINVEARTRS SNVTFKPPVT HDMVRSLFDP TLKKSLLEKC 

        70         80         90        100        110        120 
IALAIISNFF ICYWVFQRFG LQFTKYFFLV QYLFWRIAYN LGIGLVLHYQ SHYETLTNCA 

       130        140        150        160        170        180 
KTHAIFSKIP QNKDANSNFS TNSNSFSEKF WNFIRKFCQY EIRSKMPKEY DLFAYPEEIN 

       190        200        210        220        230        240 
VWLIFRQFVD LILMQDFVTY IIYVYLSIPY SWVQIFNWRS LLGVILILFN IWVKLDAHRV 

       250        260        270        280        290        300 
VKDYAWYWGD FFFLEESELI FDGVFNISPH PMYSIGYLGY YGLSLICNDY KVLLVSVFGH 

       310        320        330        340        350        360 
YSQFLFLKYV ENPHIERTYG DGTDSDSQMN SRIDDLISKE NYDYSRPLIN MGLSFNNFNK 

       370        380        390        400        410        420 
LRFTDYFTIG TVAALMLGTI MNARFINLNY LFITVFVTKL VSWLFISTIL YKQSQSKWFT 

       430        440        450        460        470        480 
RLFLENGYTQ VYSYEQWQFI YNYYLVLTYT LMIIHTGLQI WSNFSNINNS QLIFGLILVA 

       490        500        510        520        530        540 
LQTWCDKETR LAISDFGWFY GDFFLSNYIS TRKLTSQGIY RYLNHPEAVL GVVGVWGTVL 

       550        560        570        580        590        600 
MTNFAVTNII LAVLWTLTNF ILVKFIETPH VNKIYGKTKR VSGVGKTLLG LKPLRQVSDI 

       610        620        630        640        650        660 
VNRIENIIIK SLVDESKNSN GGAELLPKNY QDNKEWNILI QEAMDSVATR LSPYCELKIE 

       670        680        690        700        710        720 
NEQVETNFVL PTPVTLNWKM PIELYNGDDW IGLYKVIDTR ADREKTRVGS GGHWSATSKD 

       730        740        750        760        770        780 
SYMNHGLRHK ESVTEIKATE KYVQGKVTFD TSLLYFENGI YEFRYHSGNS HKVLLISTPF 

       790        800        810        820        830        840 
EISLPVLNTT TPELFEKDLT EFLTKVNVLK DGKFRPLGNK FFGMDSLKQL IKNSIGVELS 

       850        860 
SEYMRRVNGD AHVISHRAWD IKQTLDSLA 

P05374 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by ch flag SIB Switzerland Mirror sites: Australia  Brazil  Canada  China  Korea
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!