ID   CR1AC_BACTK             Reviewed;        1178 AA.
AC   P05068;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   04-NOV-2008, entry version 59.
DE   RecName: Full=Pesticidal crystal protein cry1Ac;
DE   AltName: Full=Insecticidal delta-endotoxin CryIA(c);
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=133 kDa crystal protein;
GN   Name=cry1Ac; Synonyms=cry1A(c), cry218, cryIA(c);
OS   Bacillus thuringiensis subsp. kurstaki.
OG   Plasmid 75 Kb.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=29339;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=HD-73;
RX   MEDLINE=86083171; PubMed=3000881; DOI=10.1016/0378-1119(85)90184-2;
RA   Adang M.J., Staver M.J., Rocheleau T.A., Leighton J., Barker R.F.,
RA   Thompson D.V.;
RT   "Characterized full-length and truncated plasmid clones of the crystal
RT   protein of Bacillus thuringiensis subsp. kurstaki HD-73 and their
RT   toxicity to Manduca sexta.";
RL   Gene 36:289-300(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=HD-73;
RX   MEDLINE=97158465; PubMed=9003589; DOI=10.1007/s002849900154;
RA   Omolo E.O., James M.D., Osir E.O., Thomson J.A.;
RT   "Cloning and expression of a Bacillus thuringiensis (L1-2) gene
RT   encoding a crystal protein active against Glossina morsitans morsitans
RT   and Chilo partellus.";
RL   Curr. Microbiol. 34:118-121(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=HD-73;
RA   Herrera G., James M.D.;
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSIR732;
RA   Gleave A.P., Hedges R.J., Broadwell A.H., Wigley P.J.;
RT   "Cloning and nucleotide sequence of an insecticidal crystal protein
RT   gene from Bacillus thuringiensis DSIR732 active against 3 species of
RT   leafroller (Lepidoptera, Tortricidae).";
RL   N. Z. J. Crop Hortic. Sci. 20:27-36(1992).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=YBT-1520;
RA   Sun M., Yu Z.;
RL   Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of many lepidopteran larvae.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during
CC       sporulation and is accumulated both as an inclusion and as part of
CC       the spore coat.
CC   -!- BIOTECHNOLOGY: Introduced by genetic manipulation and expressed in
CC       insect-resistant cotton and tomato by Calgene (Monsanto) and in
CC       maize by Dekalb Genetics.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; M11068; AAA22331.1; -; Genomic_DNA.
DR   EMBL; U87397; AAC44841.1; -; Genomic_DNA.
DR   EMBL; U87793; AAB46989.1; -; Genomic_DNA.
DR   EMBL; U89872; AAB49768.1; -; Genomic_DNA.
DR   EMBL; AJ002514; CAA05505.1; -; Genomic_DNA.
DR   PIR; A23962; USBSXH.
DR   HSSP; P02965; 1CIY.
DR   GO; GO:0030435; P:sporulation; IEA:UniProtKB-KW.
DR   InterPro; IPR005638; Endotoxin_C.
DR   InterPro; IPR001178; Endotoxin_M.
DR   InterPro; IPR015790; Endotoxin_m_sub_1.
DR   InterPro; IPR005639; Endotoxin_N.
DR   Gene3D; G3DSA:2.100.10.10; Endotoxin_M; 1.
DR   Gene3D; G3DSA:1.20.190.10; Endotoxin_N; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
PE   1: Evidence at protein level;
KW   Genetically modified food; Plasmid; Sporulation; Toxin.
FT   CHAIN         1   1178       Pesticidal crystal protein cry1Ac.
FT                                /FTId=PRO_0000174024.
SQ   SEQUENCE   1178 AA;  133331 MW;  9B34F2ED410E88BB CRC64;
     MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF VPGAGFVLGL
     VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL EGLSNLYQIY AESFREWEAD
     PTNPALREEM RIQFNDMNSA LTTAIPLFAV QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ
     RWGFDAATIN SRYNDLTRLI GNYTDYAVRW YNTGLERVWG PDSRDWVRYN QFRRELTLTV
     LDIVALFPNY DSRRYPIRTV SQLTREIYTN PVLENFDGSF RGSAQGIERS IRSPHLMDIL
     NSITIYTDAH RGYYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI VAQLGQGVYR
     TLSSTLYRRP FNIGINNQQL SVLDGTEFAY GTSSNLPSAV YRKSGTVDSL DEIPPQNNNV
     PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA PMFSWIHRSA EFNNIIASDS ITQIPAVKGN
     FLFNGSVISG PGFTGGDLVR LNSSGNNIQN RGYIEVPIHF PSTSTRYRVR VRYASVTPIH
     LNVNWGNSSI FSNTVPATAT SLDNLQSSDF GYFESANAFT SSLGNIVGVR NFSGTAGVII
     DRFEFIPVTA TLEAEYNLER AQKAVNALFT STNQLGLKTN VTDYHIDQVS NLVTYLSDEF
     CLDEKRELSE KVKHAKRLSD ERNLLQDSNF KDINRQPERG WGGSTGITIQ GGDDVFKENY
     VTLSGTFDEC YPTYLYQKID ESKLKAFTRY QLRGYIEDSQ DLEIYLIRYN AKHETVNVPG
     TGSLWPLSAQ SPIGKCGEPN RCAPHLEWNP DLDCSCRDGE KCAHHSHHFS LDIDVGCTDL
     NEDLGVWVIF KIKTQDGHAR LGNLEFLEEK PLVGEALARV KRAEKKWRDK REKLEWETNI
     VYKEAKESVD ALFVNSQYDQ LQADTNIAMI HAADKRVHSI REAYLPELSV IPGVNAAIFE
     ELEGRIFTAF SLYDARNVIK NGDFNNGLSC WNVKGHVDVE EQNNQRSVLV VPEWEAEVSQ
     EVRVCPGRGY ILRVTAYKEG YGEGCVTIHE IENNTDELKF SNCVEEEIYP NNTVTCNDYT
     VNQEEYGGAY TSRNRGYNEA PSVPADYASV YEEKSYTDGR RENPCEFNRG YRDYTPLPVG
     YVTKELEYFP ETDKVWIEIG ETEGTFIVDS VELLLMEE
//