[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=HD-73; DOI=10.1016/0378-1119(85)90184-2; PubMed=3000881 [NCBI, ExPASy, EBI, Israel, Japan] Adang M.J., Staver M.J., Rocheleau T.A., Leighton J., Barker R.F., Thompson D.V.; "Characterized full-length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis subsp. kurstaki HD-73 and their toxicity to Manduca sexta."; Gene 36:289-300(1985).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=HD-73; DOI=10.1007/s002849900154; PubMed=9003589 [NCBI, ExPASy, EBI, Israel, Japan] Omolo E.O., James M.D., Osir E.O., Thomson J.A.; "Cloning and expression of a Bacillus thuringiensis (L1-2) gene encoding a crystal protein active against Glossina morsitans morsitans and Chilo partellus."; Curr. Microbiol. 34:118-121(1997).
[3]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=HD-73; Herrera G., James M.D.; Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
[4]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=DSIR732; Gleave A.P., Hedges R.J., Broadwell A.H., Wigley P.J.; "Cloning and nucleotide sequence of an insecticidal crystal protein gene from Bacillus thuringiensis DSIR732 active against 3 species of leafroller (Lepidoptera, Tortricidae)."; N. Z. J. Crop Hortic. Sci. 20:27-36(1992).
[5]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=YBT-1520; Sun M., Yu Z.; Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.
DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.
BIOTECHNOLOGY: Introduced by genetic manipulation and expressed in insect-resistant cotton and tomato by Calgene (Monsanto) and in maize by Dekalb Genetics.
MISCELLANEOUS: Toxic segment of the protein is located in the N-terminus.
SIMILARITY: Belongs to the delta endotoxin family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M11068; AAA22331.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U87397; AAC44841.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U87793; AAB46989.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U89872; AAB49768.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ002514; CAA05505.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A23962; USBSXH.

3D structure databases

HSSP

P02965; 1CIY. [HSSP ENTRY / PDB]

ModBase

P05068.

Family and domain databases

InterPro

IPR005638; Endotoxin_C.
IPR001178; Endotoxin_M.
IPR015790; Endotoxin_m_sub_1.
IPR005639; Endotoxin_N.
Graphical view of domain structure.

Gene3D

G3DSA:2.100.10.10; Endotoxin_M; 1.
G3DSA:1.20.190.10; Endotoxin_N; 1.

Pfam

PF03944; Endotoxin_C; 1.
PF00555; Endotoxin_M; 1.
PF03945; Endotoxin_N; 1.
Pfam graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Genetically modified food; Plasmid; Sporulation; Toxin.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 1178`	`1178`		`Pesticidal crystal protein cry1Ac.`	`PRO_0000174024`

Sequence information

Length: 1178 AA [This is the length of the unprocessed precursor]

Molecular weight: 133331 Da [This is the MW of the unprocessed precursor]

CRC64: 9B34F2ED410E88BB [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF VPGAGFVLGL 

        70         80         90        100        110        120 
VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL EGLSNLYQIY AESFREWEAD 

       130        140        150        160        170        180 
PTNPALREEM RIQFNDMNSA LTTAIPLFAV QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ 

       190        200        210        220        230        240 
RWGFDAATIN SRYNDLTRLI GNYTDYAVRW YNTGLERVWG PDSRDWVRYN QFRRELTLTV 

       250        260        270        280        290        300 
LDIVALFPNY DSRRYPIRTV SQLTREIYTN PVLENFDGSF RGSAQGIERS IRSPHLMDIL 

       310        320        330        340        350        360 
NSITIYTDAH RGYYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI VAQLGQGVYR 

       370        380        390        400        410        420 
TLSSTLYRRP FNIGINNQQL SVLDGTEFAY GTSSNLPSAV YRKSGTVDSL DEIPPQNNNV 

       430        440        450        460        470        480 
PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA PMFSWIHRSA EFNNIIASDS ITQIPAVKGN 

       490        500        510        520        530        540 
FLFNGSVISG PGFTGGDLVR LNSSGNNIQN RGYIEVPIHF PSTSTRYRVR VRYASVTPIH 

       550        560        570        580        590        600 
LNVNWGNSSI FSNTVPATAT SLDNLQSSDF GYFESANAFT SSLGNIVGVR NFSGTAGVII 

       610        620        630        640        650        660 
DRFEFIPVTA TLEAEYNLER AQKAVNALFT STNQLGLKTN VTDYHIDQVS NLVTYLSDEF 

       670        680        690        700        710        720 
CLDEKRELSE KVKHAKRLSD ERNLLQDSNF KDINRQPERG WGGSTGITIQ GGDDVFKENY 

       730        740        750        760        770        780 
VTLSGTFDEC YPTYLYQKID ESKLKAFTRY QLRGYIEDSQ DLEIYLIRYN AKHETVNVPG 

       790        800        810        820        830        840 
TGSLWPLSAQ SPIGKCGEPN RCAPHLEWNP DLDCSCRDGE KCAHHSHHFS LDIDVGCTDL 

       850        860        870        880        890        900 
NEDLGVWVIF KIKTQDGHAR LGNLEFLEEK PLVGEALARV KRAEKKWRDK REKLEWETNI 

       910        920        930        940        950        960 
VYKEAKESVD ALFVNSQYDQ LQADTNIAMI HAADKRVHSI REAYLPELSV IPGVNAAIFE 

       970        980        990       1000       1010       1020 
ELEGRIFTAF SLYDARNVIK NGDFNNGLSC WNVKGHVDVE EQNNQRSVLV VPEWEAEVSQ 

      1030       1040       1050       1060       1070       1080 
EVRVCPGRGY ILRVTAYKEG YGEGCVTIHE IENNTDELKF SNCVEEEIYP NNTVTCNDYT 

      1090       1100       1110       1120       1130       1140 
VNQEEYGGAY TSRNRGYNEA PSVPADYASV YEEKSYTDGR RENPCEFNRG YRDYTPLPVG 

      1150       1160       1170 
YVTKELEYFP ETDKVWIEIG ETEGTFIVDS VELLLMEE

P05068 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools