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UniProtKB/Swiss-Prot entry P04972


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CNRG_BOVIN
Primary accession number P04972
Secondary accession numbers None
Integrated into Swiss-Prot on August 13, 1987
Sequence was last modified on August 13, 1987 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 67)
Name and origin of the protein
Protein name Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma
Synonyms GMP-PDE gamma
EC 3.1.4.17
Gene name
Name: PDE6G
Synonyms: PDEG
From
Bos taurus (Bovine) [TaxID: 9913] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos.
Protein existence 1: Evidence at protein level;
References
[1]
PROTEIN SEQUENCE.
TISSUE=Retina;
PubMed=3011376 [NCBI, ExPASy, EBI, Israel, Japan]
Ovchinnikov Y.A., Muradov K.G., Feigina M.Y., Nazimov I.V., Khoroshilova N.I.;
"Cyclic GMP-phosphodiesterase from the cattle retina. Amino acid sequence of the gamma subunit.";
Dokl. Akad. Nauk SSSR 287:1496-1498(1986).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Retina;
Ovchinnikov Y.A., Gubanov V.V., Khramtsov N.V., Akhmedov N.B., Zagranichny V.E., Ishchenko K.A., Muradov K.G., Barinov A.A., Bondarenko V.A., Kumarev V.P., Kobzev V.F., Lipkin V.M.;
"Cyclic GMP phosphodiesterase from bovine retina. Nucleotide sequence of cDNA of the gamma-subunit.";
Dokl. Biochem. 288:216-218(1986).
[3]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Retina;
DOI=10.1016/0014-5793(86)80830-4; PubMed=3015681 [NCBI, ExPASy, EBI, Israel, Japan]
Ovchinnikov Y.A., Lipkin V.M., Kumarev V.P., Gubanov V.V., Khramtsov N.V., Akhmedov N.B., Zagranichny V.E., Muradov K.G.;
"Cyclic GMP phosphodiesterase from cattle retina. Amino acid sequence of the gamma-subunit and nucleotide sequence of the corresponding cDNA.";
FEBS Lett. 204:288-292(1986).
[4]
X-RAY CRYSTALLOGRAPHY (2.02 ANGSTROMS) OF 46-87 IN COMPLEX WITH RGS9 AND GNAT1.
DOI=10.1038/35059138; PubMed=11234020 [NCBI, ExPASy, EBI, Israel, Japan]
Slep K.C., Kercher M.A., He W., Cowan C.W., Wensel T.G., Sigler P.B.;
"Structural determinants for regulation of phosphodiesterase by a G protein at 2.0 A.";
Nature 409:1071-1077(2001).
Comments
  • FUNCTION: Participates in processes of transmission and amplification of the visual signal. cGMP-PDEs are the effector molecules in G-protein-mediated phototransduction in vertebrate rods and cones.
  • CATALYTIC ACTIVITY: Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.
  • SUBUNIT: Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.
  • SIMILARITY: Belongs to the rod/cone cGMP-PDE gamma subunit family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X04823; CAA28507.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X04270; CAA27821.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A25268; A25268.
RefSeq NP_776846.1; -.
UniGene Bt.54
3D structure databases
PDB
1FQJ; X-ray; 2.02 A; C=46-87.[ExPASy / RCSB / EBI]
2JU4; NMR; -; A=1-86.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1FQJ; -.
2JU4; -.
DisProt DP00347; -.
ModBase P04972.
Ontologies
GO
GO:0050896; Biological process: response to stimulus (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR006952; PDE6_gamma.
Graphical view of domain structure.
PANTHER PTHR12122; PDE6_gamma; 1.
Pfam PF04868; PDE6_gamma; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000969; 35-cGMP_Pdiase_g; 1.
BLOCKS P04972.
ProtoNet P04972.
Genome annotation databases
Ensembl ENSBTAG00000000354; Bos taurus. [Contig view]
GeneID 281977; -.
KEGG bta:281977; -.
Phylogenomic databases
HOVERGEN P04972; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Acetylation; cGMP; Direct protein sequencing; Hydrolase; Sensory transduction; Vision.
Features
SEVIEWER logo Feature table viewer
KeyFrom  To Length Description FTId
CHAIN   1   87  87     Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma. PRO_0000166113
COMPBIAS   24   45  22     Arg/Lys-rich (basic). 
MOD_RES   1    1        N-acetylmethionine. 
HELIX   62   65  4      
HELIX   69   73  5      
HELIX   78   83  6      
Sequence information
Length: 87 AA [This is the length of the unprocessed precursor] Molecular weight: 9669 Da [This is the MW of the unprocessed precursor] CRC64: 3AA2D6B5D7D14D58 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MNLEPPKAEI RSATRVMGGP VTPRKGPPKF KQRQTRQFKS KPPKKGVQGF GDDIPGMEGL 

        70         80 
GTDITVICPW EAFNHLELHE LAQYGII 

P04972 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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